Evaluation of hydroxyapatite–bioglass and hydroxyapatite–ethyl vinyl acetate composite extracts on antioxidant defense mechanism and genotoxicity: Anin vitrostudy

Abstract

Hydroxyapatite-bioglass (HA BG) and hydroxyapatite-ethyl vinyl acetate (HA EVA) are two composite materials that have been developed for bone substitution. Their activity on antioxidant defense mechanism and genotoxicity has not been investigated before. To further confirm its biocompatibility, the present study was undertaken to investigate the effect of HA BG and HA EVA on mice liver antioxidant mechanism along with chromosomal aberrations in human lymphocytes. Physiological saline extract of HA BG and HA EVA showed no adverse effect on liver antioxidant mechanism compared to the cyclophosphamide (CP)-induced toxicity on mice liver homogenate. The results were judged from the in vitro studies made on reduced glutathione, glutathione reductase and lipid peroxidation. These results were well supported by CP- and mytomycin C (MC)-induced genotoxicity studies on human lymphocytes in the presence and absence of a metabolic activator (S9). Hence, it was suggested that these tests could be considered for preliminary toxicological screening of materials intended for clinical applications ahead of in vivo animal model evaluation.

New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes

“…It does not con-tain any protein and does not cause any allergic or immune reaction (Hu et al 2011). However, HA has intrinsically poor mechanical properties (Arun et al 2011), which can be improved by combining it with newly synthesized nanomaterials. To improve its characteristics and produce the new endodontic material, the mixture of HA with CS was formulated.…”

Smart behavior of elastomeric composites produced by injection molding

“…As it is a very important member of the family of engineering polymers, its physical and thermomechanical properties have been studied extensively and are well documented . Since EVA is also biocompatible and has been used in many biomedical engineering applications, such as in drug delivery devices and its composites are often employed in the field of shape memory materials, like in heat‐shrinking packaging . In this optic it is very important to produce EVA based composites stimuli responsive.…”

Long Term Effect of Biodegradable Polymer on Oxidative

“…In order to determine the GSH level, a sub sample of tissue was thawed, and homogenized using 0.1 M phosphate buffer. GSH contents were estimated by Ellman's reaction using DTNB as described elsewhere [20] with slight modification [21].…”

“…1) Preparation of material extract and peripheral blood lymphocyte culture: Material extract preparation and peripheral blood lymphocyte culture was performed as described elsewhere [21] with slight modification. Cyclophosphamide (100 μg/culture) alone was used as positive control with and without the metabolic activator S9.…”