The purpose of this research was to study the impact of N levels applied with proline as well as plant distance on the vegetative growth, chemical content, yield, yield components, and quality attributes of maize under different irrigation intervals. The research was undertaken at an experimental field of Agricultural Faculty, El-Mansoura University during 2018-2019. The results showed that application of 150% nitrogen fertilization from recommended dose in presence of 50 mg/l proline significantly increased growth parameters (plant length, fresh and dry weight of flag, leaves area), chlorophyll content, N, P, K and proline content of maize leaves as well as yield attributed (ear length, 1000-graine weight, grain yield and straw yield) and quality of grains (crude proline, fiber, total carbohydrates and oil%). All parameters under investigation recorded high significant values with plant spacing 15 cm. As for irrigation intervals the results revealed that 11 days' intervals were the most suitable for previous maize parameters. So, it could be recommended touse150 N-fertilization in presence of proline + 15 cm plant spacing and 11 days' irrigation intervals.