Evaluation of Fatty Acid Extraction Methods for Thraustochytrium sp. ONC-T18

Abstract

Various extraction methods were assessed in their capacity to extract fatty acids from a dried biomass of Thraustochytrium sp. ONC-T18. Direct saponification using KOH in ethanol or in hexane:ethanol was one of the most efficient techniques to extract lipids (697 mg g(-1)). The highest amount of fatty acids (714 mg g(-1)) was extracted using a miniaturized Bligh and Dyer extraction technique. The use of ultrasonics to break down cell walls while extracting with solvents (methanol:chloroform) also offered high extraction yields of fatty acids (609 mg g(-1)). Moreover, when the transesterification mixture used for a direct transesterification method was doubled, the extraction of fatty acids increased approximately 77% (from 392 to 696 mg g(-1)). This work showed that Thraustochytrium sp. ONC-T18 has the ability to produce over 700 mg g(-1) of lipids, including more than 165 mg g(-1) of docosahexaenoic acid, which makes this microorganism a potential candidate for the commercial production of polyunsaturated fatty acids. Finally, other lipids, such as myristic, palmitic, palmitoleic, and oleic acids, were also produced and recovered in significant amounts (54, 196, 123, and 81 mg g(-1)), respectively.

Comparison of two lipid extraction methods produced by yeast in cheese whey

“…Cescut et al (2011) compared the pressurized liquid extraction with classical methods of Bligh and Dyer modified and Soxhlet extraction for strain of Rhodotorula glutinis, they found no significant difference in extraction efficiency between the pressurized liquid extraction with the method of Bligh and Dyer modified, although both were higher efficient than Soxhlet apparatus. Burja et al (2007) studied various methods for extracting fatty acids from Thraustochytrium sp. ONC-T18 and they obtained larger amounts of fatty acids using the method of Bligh and Dyer miniaturized.…”

Prevalence of PUFA Rich Thraustochytrids sps. along the Coast of Mumbai for Production of Bio Oil

“…Total lipid content was calculated using a modified miniaturized Bligh-Dyer method (Burja, 2007) where in 125 mg of dried cells were mixed with 6.25 ml chloroform, 12.5 ml methanol, and 5 ml of 50 mM K 2 HPO 4 buffer solution (pH 7.4). Samples were agitated for 1 h, at 200 rpm, at 28°C, after which further 6.25 ml chloroform 12.5 ml methanol and 6.25 ml phosphate buffer was added to each sample.…”

Characterization and Comparison of Biodiesels Made from Korean Freshwater Algae

“…The dried extract was treated with a premade solution of methanol and potassium hydroxide to facilitate transesterification. 19 Twenty milliliters of hexane was also added to the mixture to help isolate the biodiesel. The whole mixture was heated to 30 o C and stirred for 10 h. After 10 h, the mixture was cooled and the methanol and hexane layers were separated.…”