Characterization and Comparison of Biodiesels Made from Korean Freshwater Algae

Yeo, In-Joon; Jeong, Ji-Eun; Cho, Yun-Ju; Hong, Ji Won; Yoon, Ho-Sung; Kim, Sung-Hong; Kim, Sunghwan

doi:10.5012/bkcs.2011.32.8.2830

Cited by 10 publications

(8 citation statements)

References 17 publications

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“…To simulate the commercial production of microalgaebased biofuels and to obtain sufficient biomass for analysis, strain KNUA026 was inoculated into 16 L of BG-11 ( ) with a light:dark cycle of 16 : 8 h. After incubating for 21 days, cyanobacterial cells were harvested and the fatty acid composition was analyzed by GC/MS (GCMS-QP2010 ultra; Shimadzu, Tokyo, Japan) according to our previous publication (Yeo et al 2011). …”

Section: Lipid Extraction and Gas Chromatography / Mass Spectrometry mentioning

confidence: 99%

Natural production of alkane by an easily harvested freshwater cyanobacterium, Phormidium autumnale KNUA026

Chang¹,

Hong²,

Chae³

et al. 2013

ALGAE

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A freshwater cyanobacterium, Phormidium autumnale KNUA026, was isolated from puddles of icy water in Gyeongsan City, South Korea and its potential as a biofuel feedstock was investigated. Maximal growth was obtained when the culture was incubated at 25°C and around pH 9.0. The total lipid content of the isolate was approximately 14.0% of dry weight and it was found that strain KNUA026 was able to autotrophically synthesize heptadecane (C 17 H 36 ) which can be directly used as fuel without requiring a transesterification step. As this benthic cyanobacterium was capable of forming thick mats, it could be easily harvested by gravitational settling and this property may reduce the cost of production in commercial applications. Hence, P. autumnale KNUA026 appears to be a promising resource for use in the production of microalgae-based biofuels.Key Words: algae-based fuel; alkane; cyanobacterium; heptadecane; Phormidium autumnale INTRODUCTIONIn recognition of the current energy crisis, alternative energy sources are currently being explored and developed at an increasing pace; biofuels in particular have received a considerable amount of attention over the past few years. Conventional biofuels, such as bioethanol and biodiesel, are produced primarily from cereal crops and oil seeds. However, the mass production of crop-based biofuels has resulted in serious side effects, such as increases in food prices, deforestation, and carbon emissions (Sims et al. 2010). Thus, algae-based biofuels have been recognized as an attractive option, as they use minimal land resources (Chisti 2007) and do not compete with food production (Huang et al. 2010). Additionally, algaebased biofuels have several other advantages, including rapid growth rates (Schenk et al. 2008), higher lipid contents (Hu et al. 2008), and higher CO 2 uptake rates (Jorquera et al. 2010) relative to other energy crops. Considering these advantages, microalgae biofuels have been recognized as the only current renewable source of liquid transportation fuel which is compatible with the existing engines and distribution systems (Schenk et al. 2008). Microalgae, including cyanobacteria, have been reported to generate a variety of lipids, hydrocarbons, fatty alcohols, and other complex oils ( ), until growth was apparent. An aliquot of the brown-colored biomass was taken by pipetting, and was sonicated for approximately 3-5 s using an ultrasonic cell disruptor (Model 550; Fisher Scientific, Pittsburgh, PA, USA). The filaments were then transferred onto BG-11 agar plates containing 100 µg mL -1 of meropenem (Yuhan Pharmaceuticals, Ochang, Korea) and were incubated in the dark for 24 h to eliminate contaminating bacteria (Choi et al. 2008). The culture was then inoculated onto fresh BG-11 agar plates and incubated for 14 days under a light : dark cycle (16 : 8 h) at 15°C. Morphological and molecular identificationThe isolate was grown in BG-11(+) medium for 21 days. Live cells were harvested by centrifugation at 3,000 ×g for 5 min, washed with sterile distilled water, ...

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Section: Lipid Extraction and Gas Chromatography / Mass Spectrometry mentioning

confidence: 99%

Natural production of alkane by an easily harvested freshwater cyanobacterium, Phormidium autumnale KNUA026

Chang¹,

Hong²,

Chae³

et al. 2013

ALGAE

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“…with a light : dark cycle of 16 : 8 h. After incubating for 30 days, algal cells were harvested by centrifugation at 3,220 ×g (Centrifuge 5810R; Eppendorf, Hamburg, Germany) for 10 min. The harvested algal biomass was freeze-dried and mixed with chloroform-methanol (2 : 1) overnight according to the method described by Yeo et al (2011). The chloroform extract was isolated and dried in a rotary evaporator (RV10; IKA, Wilmington, NC, USA).…”

Section: Lipid Extraction and Gas Chromatography/mass Spectrometry (Gmentioning

confidence: 99%

Isolation and description of a Korean microalga, Asterarcys quadricellulare KNUA020, and analysis of its biotechnological potential

Hong¹,

Kim²,

Chang³

et al. 2012

ALGAE

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A eukaryotic microalga, Asterarcys quadricellulare KNUA020, was isolated from garden soil at Kyungpook National University in Daegu, South Korea and its biotechnological potential was assessed. Optimal growth was obtained when the culture was incubated at 25°C and around pH 7.0. The total lipid content of the isolate was 15.5% of dry weight and its most abundant fatty acid was nutritionally important C18:3 ω3 (α-linolenic acid, ALA). In addition, a high-value fatty alcohol, hexadecenol (C 20 H 40 O), was also identified in this photosynthetic microorganism. Hence, A. quadricellulare KNUA020 appears to be promising for use in the production of microalgae-based biochemicals.

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“…The isolates were autotrophically grown in BG-11 medium for 20 days and cells were harvested by centrifugation. Then, the samples were freeze-dried and total lipids were extracted by a modified Bligh and Dyer method (Yeo et al 2011). The fatty acid methyl ester composition was analyzed using a 6890N gas chromatograph (Agilent, Santa Clara, CA, USA) equipped with a 5973N mass selective detector, and an HP-5MS capillary column (30 m × 0.25 mm ID × 0.25 μm film thickness).…”

Section: Gas Chromatography/mass Spectrometry (Gc/ms) Analysismentioning

confidence: 99%

Phylogeny, morphology, and physiology of Micractinium strains isolated from shallow ephemeral freshwater in Antarctica

Hong

Cho

et al. 2015

Phycological Research

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SUMMARYCryotolerant eukaryotic microalgae were isolated from meltwater streams on Ardley Island and King George Island in Antarctica, and their morphological, molecular, and physiological characteristics were investigated. Owing to their simple morphology, distinctive characters were not observed with neither light microscopy nor transmission electron microscopy. However, molecular phylogenetic inferences drawn from the concatenated small subunit rRNA and internal transcribed spacer sequence data indicated that these microalgal strains belonged to the genus Micractinium. All the Micractinium strains showed cryotolerant properties, while their optimum growth temperature was around 20°C. Similar to other cryotolerant organisms, these Antarctic microalgae also contained a higher ratio of polyunsaturated fatty acids to saturated fatty acids. In this study, new Antarctic Micractinium spp. were discovered and added to the culture collection. These cryotolerant strains may serve as a promising source of nutritionally important linoleic (C18:2 ω6) and α-linolenic (C18:3 ω3) acids.

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Characterization and Comparison of Biodiesels Made from Korean Freshwater Algae

Cited by 10 publications

References 17 publications

Natural production of alkane by an easily harvested freshwater cyanobacterium, Phormidium autumnale KNUA026

Natural production of alkane by an easily harvested freshwater cyanobacterium, Phormidium autumnale KNUA026

Isolation and description of a Korean microalga, Asterarcys quadricellulare KNUA020, and analysis of its biotechnological potential

Phylogeny, morphology, and physiology of Micractinium strains isolated from shallow ephemeral freshwater in Antarctica

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