Evaluation of enzyme-linked immunosorbent assay using milk samples as a potential screening test of bovine tuberculosis of dairy cows in Korea

Jeon, Bo Young; Kim, Seung Cheol; Je, Sungmo; Kwak, Jeong-Yeon; Cho, Jang Eun; Woo, Jong Tae; Seo, Sangkyo; Shim, Hang Sub; Park, Byoung Ok; Lee, Sung-Sik; Cho, Sang Nae

doi:10.1016/j.rvsc.2009.12.002

Cited by 22 publications

(18 citation statements)

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“…The test sensitivity for the milk samples was lower than the 88% sensitivity reported by Jeon et al (8), although the TB status of the animals in that study was not confirmed by slaughter of the animals, and the tuberculin skin test was used as the reference test. However, the test sensitivity in the current study was comparable to the 40% sensitivity reported for serum samples from M. bovisinfected animals from New Zealand tested by the IDEXX test in another study (6).…”

Section: Resultscontrasting

confidence: 60%

“…An economic analysis of the control strategies for bovine TB surveillance indicated that enzyme-linked immunosorbent assay (ELISA) testing of bulk milk samples may be a cost-effective strategy if the testing became feasible (7). Encouraging results for the detection of antibodies to M. bovis in individual and bulk milk samples were recently reported (6,8), and the detection of antibodies in bulk milk samples has been used in control programs for the diagnosis of brucellosis, enzootic bovine leukosis, and Johne's disease in cattle (9)(10)(11). However, one of the concerns with the use of serological tests for the detection of M. bovis infection in cattle has been the variation in the sensitivities of tests when applied to sera from M. bovis-infected cattle from different countries.…”

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confidence: 99%

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Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay for the Diagnosis of Bovine Tuberculosis from Milk Samples from Dairy Cows

Buddle

Wilson

Luo

et al. 2013

Clin Vaccine Immunol

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Milk samples from dairy cows provide a ready source of material for measuring antibody responses to Mycobacterium bovis antigens. In this study, we evaluated the IDEXX enzyme-linked immunosorbent assay (ELISA) for the measurement of antibody responses to M. bovis antigens MPB70 and MPB83 in milk samples from New Zealand cattle. Test sensitivities for individual milk and serum samples were assessed in samples collected from 44 M. bovis-infected cows, and test specificities were assessed in milk samples collected from 356 cows from tuberculosis (TB)-free herds. Milk vat samples were collected from 505 herds from regions with relatively high or low prevalences of infection. The ELISA had a sensitivity of 50% and a specificity of 97.5% for milk samples, and the test sensitivities for milk and serum samples were the same. Dilution of the positive test milk samples in milk from noninfected cows at 1/10, 1/20, and 1/50 dilutions reduced the proportions of positive responses to 13/21, 9/21, and 4/21, respectively. Small differences were observed in the ELISA responses of milk samples from individual TB-free cows collected at different times during lactation. No significant differences were detected in the ELISA responses of milk vat samples collected from infected and noninfected herds. This study shows that milk samples can be substituted for serum samples for screening individual cows for M. bovis infection, and pooling of milk samples from 10 to 20 animals can result in a reduction in the sensitivity by approximately 50%. However, screening of milk vat samples is unlikely to be useful in countries with low prevalences of M. bovis in cattle and large herd sizes. Bovine tuberculosis (TB) caused by Mycobacterium bovis is a major economic problem in many countries, and control of this chronic disease in domestic livestock has often proved difficult. Although the disease has been eradicated from cattle in a number of countries through the implementation of a "test-andslaughter" control program, this strategy has been less effective in countries which have a wildlife reservoir of M. bovis infection and has been impractical in countries where the strategy is unaffordable or socially unacceptable (1). The test-and-slaughter programs have been based on screening of animals using the tuberculin skin test, which measures delayed hypersensitivity responses to purified protein derivative (PPD) prepared from M. bovis or which, in some countries, compares PPDs prepared from M. bovis and Mycobacterium avium. More recently, the whole-blood gamma interferon (IFN-␥) test has been used in conjunction with the skin test to improve specificities by retesting skin test reactor cattle or to improve sensitivities by retesting skin test-negative animals from chronically infected herds (2, 3). Although the IFN-␥ test has made a valuable contribution in improving the accuracy of diagnosing TB in cattle, additional types of assays are needed, particularly assays which are inexpensive and which can potentially be used on pooled samples from group...

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Section: Resultscontrasting

confidence: 60%

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confidence: 99%

Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay for the Diagnosis of Bovine Tuberculosis from Milk Samples from Dairy Cows

Buddle

Wilson

Luo

et al. 2013

Clin Vaccine Immunol

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“…As the use of bulk-tank milk samples for screening is less labor-intensive and more cost-effective than individual-tank sampling [19], this approach represents an important development in dairy research.…”

Section: Resultsmentioning

confidence: 99%

Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil

et al. 2013

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BackgroundMycobacterium spp. is one of the most important species of zoonotic pathogens that can be transmitted from cattle to humans. The presence of these opportunistic, pathogenic bacteria in bovine milk has emerged as a public-health concern, especially among individuals who consume raw milk and related dairy products. To address this concern, the Brazilian control and eradication program focusing on bovine tuberculosis, was established in 2001. However, bovine tuberculosis continues to afflict approximately 1,3 percent of the cattle in Brazil. In the present study, 300 samples of milk from bovine herds, obtained from both individual and collective bulk tanks and informal points of sale, were cultured on Löwenstein-Jensen and Stonebrink media. Polymerase chain reaction (PCR)-based tests and restriction-enzyme pattern analysis were then performed on the colonies exhibiting phenotypes suggestive of Mycobacterium spp., which were characterized as acid-fast bacilli.ResultsOf the 300 bovine milk samples that were processed, 24 were positively identified as Mycobacterium spp.Molecular identification detected 15 unique mycobacterial species: Mycobacterium bovis, M. gordonae, M. fortuitum, M. intracellulare, M. flavescens, M. duvalii, M. haemophilum, M. immunogenum, M. lentiflavum, M. mucogenicum, M. novocastrense, M. parafortuitum, M. smegmatis, M. terrae and M. vaccae. The isolation of bacteria from the various locations occurred in the following proportions: 9 percent of the individual bulk-tank samples, 7 percent of the collective bulk-tank samples and 8 percent of the informal-trade samples. No statistically significant difference was observed between the presence of Mycobacterium spp. in the three types of samples collected, the milk production profiles, the presence of veterinary assistance and the reported concerns about bovine tuberculosis prevention in the herds.ConclusionThe microbiological cultures associated with PCR-based identification tests are possible tools for the investigation of the presence of Mycobacterium spp. in milk samples. Using these methods, we found that the Brazilian population may be regularly exposed to mycobacteria by consuming raw bovine milk and related dairy products. These evidences reinforces the need to optimize quality programs of dairy products, to intensify the sanitary inspection of these products and the necessity of further studies on the presence of Mycobacterium spp. in milk and milk-based products.

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“…27 Also, the high relationship among an antibody's reactions in milk and blood had been reported, at same animal, in bTB and John's disease. 28,29 As well as, milk might become, widely, extra-suitable after collection of it during the routine examination of herds. 30 Milk can be contributed in identifying the diseased cattle, although about 50 percentage of technique's sensitivity would decrease.…”

Section: Discussionmentioning

confidence: 99%

The clinical and serological diagnosis of Mycobacterium bovis in blood and milk serums of lactating cows by IDEXX ELISA test in Wasit and Dhi-Qar provinces/Iraq

Al-Fattli

2016

J. Contemp. Med. Sci.

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Objectives This study was conducted to disclose the specific antibodies against M. bovis of bovine tuberculosis (bTB) in blood and milk serum samples, with detection of the most prevalent clinical signs in positive cows. Methods In some rural areas of two Iraqi provinces (Wasit and Dhi-Qar), 119 lactating cows were submitted to the clinical examination with obtaining of blood and milk to tested by using the IDEXX ELISA test. Results The overall seroprevalence in blood and milk was (20.16%) and (15.12%), respectively. In Wasit, the prevalence was (22.85%) and (15.71%), while in Dhi-Qar, the prevalence was (16.32%) and (14.28%) in blood and milk, respectively. As well as, marked significant differences in seroprevalence were observed between and within the two study's provinces and samples. According to clinical examination, a significant rising (P > 0.05) was revealed in respiratory disorders, decreasing in milk production, emaciation, rough hair coat and repetitive reproductive problems, whilst a significant decreasing (P < 0.05) in persistent feces abnormalities, mastitis, lymph nodes enlargement and loss of appetite. Conclusion The study demonstrated, for the first time in Iraq, the efficient of IDEXX ELISA, as a screening test in the detection of bTB in lactating cows by using blood and milk serum samples, and the competence of milk, as sample, in exhibition of infection. Also, the study exposed the high infection rate of bTB in cows of rural areas of Wasit and Dhi-Qar provinces.

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Evaluation of enzyme-linked immunosorbent assay using milk samples as a potential screening test of bovine tuberculosis of dairy cows in Korea

Cited by 22 publications

References 21 publications

Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay for the Diagnosis of Bovine Tuberculosis from Milk Samples from Dairy Cows

Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay for the Diagnosis of Bovine Tuberculosis from Milk Samples from Dairy Cows

Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil

The clinical and serological diagnosis of Mycobacterium bovis in blood and milk serums of lactating cows by IDEXX ELISA test in Wasit and Dhi-Qar provinces/Iraq

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