Evaluation of drought-tolerance in some tropical wheat genotypes (Triticum aestivum L.) at different osmotic-stress level

Abstract

Abiotic factors, such as temperature and drought, are the main factors limiting the cultivation under the tropical condition. Two-stage experiments were conducted to examine the drought-tolerant potential of some wheat genotypes against the osmotic stress under the tropical condition at the Laboratory and Greenhouse of Hasanuddin University and Indonesian Cereal Research Institute. The experiments were arranged in a randomized block design with the split-plot pattern and respectively provided with four and three replications. The main plot was potential osmotic stress (0, -0. 33 , and -0.67 MPa) and the sub-plot was selected wheat genotypes (17 genotypes). The results indicates that based on the germination percentage, shoot/root ratio, proline content, stomatal behavior, and relative water content, the wheat lines of O/HP-78-A22-3-7, WBLL*2KURUKU, O/HP-6-A8-2-10, and O/HP-22-A27-1-10 are identified to have better drought-tolerance than the others genotypes based on the analysis of responses to parameters observed. The positively adaptive response of some tropical wheat genotypes to drought stress may be used as a potential donor for further development of drought-tolerant wheat varieties under the tropical climate in Indonesia.

Effect of Different Priming Methods on Seed Viability and Seedling Growth of Upland Rice under Drought Stress

“…): measured using the troll and Lindley method [11]. A total of 0.5 g of leaf sample was crushed in 10 ml of ninhydrin acid, 2 ml of glacial acetic acid, heated at 100 o C for 1 hour, added 4 ml of toluene, measured by spectrophotometry /Spectronic 21-D (UV spectrophotometer) at a wavelength of 520 nm.…”

“…The use of various methods is more widespread trying to see how effective it is in triggering plant tolerance to stress. To induce stress in drought experiments can be done by manipulating water application (irrigation intervals, field capacity), or in some studies, drought stress is obtained from differences in osmosis pressure levels obtained by applying PEG as conducted in [11].…”