Evaluation of doxorubicin toxicity on cardiomyocytes using a dual functional extracellular biochip

Liang, Xiao; Hu, Zhaoyin; Zhang, Wei; Wu, Chengxiong; Yu, Hui; Wang, Ping

doi:10.1016/j.bios.2010.07.093

Cited by 32 publications

(17 citation statements)

References 22 publications

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“…8–10 Several different types of cell-based biosensors have been developed for cardiotoxicity testing such as by using digital movie analysis, 11, 12 microelectrode arrays (MEA), 13, 14 and light-addressable potentiometric sensors (LAPS). 15 The movie analysis method was adapted to analyze the changes in the beating rates of heart cells through image processing.…”

Section: Introductionmentioning

confidence: 99%

A cell-based biosensor for real-time detection of cardiotoxicity using lensfree imaging

et al. 2011

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A portable and cost-effective real-time cardiotoxicity biosensor was developed using a CMOS imaging module extracted from a commercially available webcam. The detection system consists of a CMOS imaging module, a white LED and a pinhole. Real-time image processing was conducted by comparing reference and live frame images. To evaluate the engineered system, the effects of two different drugs, isoprenaline and doxorubicin, on the beating rate and beat-to-beat variations of ESC-derived cardiomyocytes were measured. The detection system was used to conclude that the beat-to-beat variability increased under treatment with both isoprenaline and doxorubicin. However, the beating rates increased upon the addition of isoprenaline but decreased for cultures supplemented with doxorubicin. Moreover, the response time for both the beating rates and the beat-to-beat variability of ESC-derived cardiomyocytes under treatment of isoprenaline was shorter than doxorubicin, although the amount of isoprenaline used in the measurement was three orders of magnitude lower than that of doxorubicin. Given its ability to perform real-time cell monitoring in a simple and inexpensive manner, the proposed system may be useful for a range of cell-based biosensing applications.

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Section: Introductionmentioning

confidence: 99%

A cell-based biosensor for real-time detection of cardiotoxicity using lensfree imaging

et al. 2011

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“…To test the performance of this heart-on-a-chip device, two typical tool drugs (verapamil and doxorubicin) were selected. The concentration of verapamil and doxorubicin were chosen based on the former study [ 24 , 25 ]. The highest concentration of verapamil was 2 μM, and then it was diluted into 1 μM, 0.5 μM, 0.25 μM, 0.125 μM, and 0.0625 μM, and we performed the drug experiments separately.…”

Section: Methodsmentioning

confidence: 99%

High-Throughput Assessment of Drug Cardiac Safety Using a High-Speed Impedance Detection Technology-Based Heart-on-a-Chip

Zhang

Wang

et al. 2016

Micromachines

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Drug cardiac safety assessments play a significant role in drug discovery. Drug-induced cardiotoxicity is one of the main reasons for drug attrition, even when antiarrhythmic drugs can otherwise effectively treat the arrhythmias. Consequently, efficient drug preclinical assessments are needed in the drug industry. However, most drug efficacy assessments are performed based on electrophysiological tests of cardiomyocytes in vitro and cannot effectively provide information on drug-induced dysfunction of cardiomyocyte beating. Here we present a heart-on-a-chip device for evaluating the drug cardiac efficacy using a high-speed impedance detection technology. Verapamil and doxorubicin were utilized to test this heart-on-a-chip, and multiple parameters of cardiomyocyte beating status are used to reveal the effects of drugs. The results show that drug efficacy or cardiotoxicity can be determined by this heart-on-a-chip. We believe this heart-on-a-chip will be a promising tool for the preclinical assessment of drug cardiac efficacy.

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“…Our collaborative efforts with Dr. Robert Langer at the Massachusetts Institute of Technology have yielded the development of sacrificial melt-spun interconnected microfibers as an artificial vascular system [39,40]. The system is based on the initial experiments by Bellan, et al that used standard sugar-based cotton candy to create an interconnected 3D microchannel network inside several materials, including polydimethylsiloxane (PDMS), epoxy, and polycaprolactone (PCL) [39].…”

Section: State Of the Art In Biosensors -Environmental And Medical Apmentioning

confidence: 99%

“…The system is based on the initial experiments by Bellan, et al that used standard sugar-based cotton candy to create an interconnected 3D microchannel network inside several materials, including polydimethylsiloxane (PDMS), epoxy, and polycaprolactone (PCL) [39]. Briefly, the microchannel network of cotton candy (attached to larger sticks of sugar in order to produce macrochannel interfaces) was embedded within the material of choice, which was then allowed to solidify.…”

Section: State Of the Art In Biosensors -Environmental And Medical Apmentioning

confidence: 99%

Biomimetic Sensors for Rapid Testing of Water Resources

Grimme¹

2013

State of the Art in Biosensors - Environmental and Medical Applications

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Evaluation of doxorubicin toxicity on cardiomyocytes using a dual functional extracellular biochip

Cited by 32 publications

References 22 publications

A cell-based biosensor for real-time detection of cardiotoxicity using lensfree imaging

A cell-based biosensor for real-time detection of cardiotoxicity using lensfree imaging

High-Throughput Assessment of Drug Cardiac Safety Using a High-Speed Impedance Detection Technology-Based Heart-on-a-Chip

Biomimetic Sensors for Rapid Testing of Water Resources

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