A cell-based biosensor for real-time detection of cardiotoxicity using lensfree imaging

Kim, Sang Bok; Bae, Hojae; Min, Jae; Moon, Sang Jun; Dokmeci, Mehmet R.; Cropek, Donald M.; Khademhosseini, Ali

doi:10.1039/c1lc20098d

Cited by 89 publications

(77 citation statements)

References 35 publications

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“…Although the microwells were monitored daily, the imaging could be automated by adding a mini-microscope or a lensless CMOS imaging platform in the incubator. 61 Most importantly, however, the device material did not require any additional surface treatment and did not show any deleterious effects on stem cell proliferation and viability, enabling further, more advanced differentiation studies. The cells were contained inside a closed, sterilized device, reducing the likelihood of contamination.…”

Section: Resultsmentioning

confidence: 99%

Polyester μ-assay chip for stem cell studies

et al. 2012

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The application of microfluidic technologies to stem cell research is of great interest to biologists and bioengineers. This is chiefly due to the intricate ability to control the cellular environment, the reduction of reagent volume, experimentation time and cost, and the high-throughput screening capabilities of microscale devices. Despite this importance, a simple-to-use microfluidic platform for studying the effects of growth factors on stem cell differentiation has not yet emerged. With this consideration, we have designed and characterized a microfluidic device that is easy to fabricate and operate, yet contains several functional elements. Our device is a simple polyester-based microfluidic chip capable of simultaneously screening multiple independent stem cell culture conditions. Generated by laser ablation and stacking of multiple layers of polyester film, this device integrates a 10 Â 10 microwell array for cell culture with a continuous perfusion system and a non-linear concentration gradient generator. We performed numerical calculations to predict the gradient formation and calculate the shear stress acting on the cells inside the device. The device operation was validated by culturing murine embryonic stem cells inside the microwells for 5 days. Furthermore, we showed the ability to maintain the pluripotency of stem cell aggregates in response to concentrations of leukemia inhibitory factor ranging from 0 to $1000 U/ml. Given its simplicity, fast manufacturing method, scalability, and the cell-compatible nature of the device, it may be a useful platform for long-term stem cell culture and studies.

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Section: Resultsmentioning

confidence: 99%

Polyester μ-assay chip for stem cell studies

et al. 2012

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“…For example, a cardiotoxicity biosensor that integrated mESC-derived cardiomyocytes with an automated imaging system to monitor changes in cell contraction rate in response to pharmacologic agents has been reported. 40 Efforts to construct stem cell biosensors have only begun to realize the potential of stem cells in diagnostics and in vitro modeling applications. Advances in microscale fabrication technologies will enable construction of more physiologically relevant, 3D structured cell and tissue biosensors.…”

Section: Engineering and Physical Sciences Principles In Stem Cell Rementioning

confidence: 99%

A Global Assessment of Stem Cell Engineering

McDevitt

Palecek

Schaffer

et al. 2014

Tissue Engineering Part A

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Over the last 2 years a global assessment of stem cell engineering (SCE) was conducted with the sponsorship of the National Science Foundation, the National Cancer Institute at the National Institutes of Health, and the National Institute of Standards and Technology. The purpose was to gather information on the worldwide status and trends in SCE, that is, the involvement of engineers and engineering approaches in the stem cell field, both in basic research and in the translation of research into clinical applications and commercial products. The study was facilitated and managed by the World Technology Evaluation Center. The process involved site visits in both Asia and Europe, and it also included several different workshops. From this assessment, the panel concluded that there needs to be an increased role for engineers and the engineering approach. This will provide a foundation for the generation of new markets and future economic growth. To do this will require an increased investment in engineering, applied research, and commercialization as it relates to stem cell research and technology. It also will require programs that support interdisciplinary teams, new innovative mechanisms for academic-industry partnerships, and unique translational models. In addition, the global community would benefit from forming strategic partnerships between countries that can leverage existing and emerging strengths in different institutions. To implement such partnerships will require multinational grant programs with appropriate review mechanisms.

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“…Other webcam applications in the healthcare domain include monitoring hand rehabilitation exercises [30] and activity monitoring systems for the elderly [15]. Recently, the webcam technology was used to create a tool to identify the effects of medications in real time on certain heart cells [16].…”

Section: Webcammentioning

confidence: 99%

Leveraging consumer sensing devices for telehealth

Dhillon

Ramos

Wünsche

et al. 2012

Proceedings of the 13th International Conference of the NZ Chapter of the ACM's Special Interest Group on Human-Computer Intera

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Home telehealth applications are increasingly gaining in popularity among patients due their promise to use healthcare resources more effectively and hence to lower costs. Commercial telehealth systems usually employ patient stations and vital sign monitoring equipment in order to fulfill the need of medical professionals by enabling close monitoring of patients with severe chronic diseases. However, due their high cost, vendor lock-in, and doctor centric design, these systems are not suitable for general healthcare applications such as improving cardiac fitness, monitoring overall health, improving muscle strength and balance of elderly, or assisting with dieting programs. We argue that many of these goals can be supported in a cost effective manner by leveraging mainstream sensing devices such as game controllers and smartphones. Open-ended web-based telehealth systems can be integrated with such devices, in order to add a social component, collect health data unobtrusively, and provide feedback and health related information. In this paper, we systematically categorise and analyse consumer-level sensing devices in terms of their potential to extend the capability of telehealth systems. We show that the devices have immense potential as tools for therapy and rehabilitation activities, diagnosis, health monitoring and social support, and we reveal opportunities for professionals in computer vision, graphics and signal processing to participate in this trend.

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A cell-based biosensor for real-time detection of cardiotoxicity using lensfree imaging

Cited by 89 publications

References 35 publications

Polyester μ-assay chip for stem cell studies

Polyester μ-assay chip for stem cell studies

A Global Assessment of Stem Cell Engineering

Leveraging consumer sensing devices for telehealth

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