Evaluation of cytotoxic effects and oxidative stress with hydroxyapatite dispersions of different physicochemical properties in rat NR8383 cells and primary macrophages

Albrecht, Catrin; Scherbart, Agnes M.; Berlo, Damiën van; Braunbarth, Carola M.; Schins, Roel P. F.; Scheel, Julia

doi:10.1016/j.tiv.2009.01.005

Cited by 45 publications

(37 citation statements)

References 51 publications

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“…Particles in the fine size range may induce more pronounced responses than in the nano-range (Rothen-Rutishauser et al 2007), and the ''ultrafine hypothesis" has been challenged previously by the experimental studies ). Albrecht et al (2009) found that cytotoxicity as observed at high concentrations did not necessarily represent a compound-related effect, and could at least partly be due to physical coverage of the cells by the test NPs. The fully understanding of how WO 3 NPs interact with hepatocytes, especially at the molecular level, is still unclear.…”

Section: Discussionmentioning

confidence: 91%

“…As a matter of fact, tungsten carbide nanoparticles (WC NPs) caused reduction in cell viability between 10 and 50% compared to controls upon particle exposure in the rainbow trout (Oncorhynchus mykiss) gill cell line, RTgill-W1 (Kühnel et al 2009). Rothen-Rutishauser et al (2007) reported that the size of nano-particles or their aggregates could be considered a potential determinant for the uptake and subsequent macrophage responses, which could explain the observed differences on LDH release at the highest concentration of NPs (Albrecht et al 2009). Particles in the fine size range may induce more pronounced responses than in the nano-range (Rothen-Rutishauser et al 2007), and the ''ultrafine hypothesis" has been challenged previously by the experimental studies ).…”

Section: Discussionmentioning

confidence: 99%

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The Risk Evaluation of Tungsten Oxide Nanoparticles in Cultured Rat Liver Cells for Its Safe Applications in Nanotechnology

Türkez

Sönmez

Turkez

et al. 2014

Braz. arch. biol. technol.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

The Risk Evaluation of Tungsten Oxide Nanoparticles in Cultured Rat Liver Cells for Its Safe Applications in Nanotechnology

Türkez

Sönmez

Turkez

et al. 2014

Braz. arch. biol. technol.

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“…49 When the intracellular ROS content of RAW-264.7 macrophages was evaluated by flow cytometry with the probe DCFH/DA, after 1 day treatment with either nano-HA or nano-SiHA, a significant increase induced by nano-HA was observed at this short time of culture. However, macrophages treated with nano-SiHA showed lower ROS values than control (Figure 3B), thus indicating a beneficial action of Si-substituted material in agreement with other studies.…”

Section: Resultsmentioning

confidence: 99%

Nanocrystalline silicon substituted hydroxyapatite effects on osteoclast differentiation and resorptive activity

Matesanz¹,

Linares

Lilue³

et al. 2014

J. Mater. Chem. B

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In the present study, the effects of nanocrystalline hydroxyapatite (nano-HA) and nanocrystalline Si-substituted hydroxyapatite (nano-SiHA) on osteoclast differentiation and resorptive activity have been evaluated in vitro using osteoclast-like cells. The action of these materials on proinflammatory and reparative macrophage populations was also studied. NanoSiHA disks delayed the osteoclast differentiation and decreased the resorptive activity of these cells on their surface, as compared to nano-HA samples, without affecting cell viability. Powdered nano-SiHA also induced an increase of the reparative macrophage population. These results along with the beneficial effects on osteoblasts previously observed with powdered nano-SiHA suggest the potential of this biomaterial for modulating the fundamental processes of bone formation and turnover, preventing bone resorption and enhancing bone formation at implantation sites in treatment of osteoporotic bone and in bone repair and regeneration.

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“…As a matter of fact, it was concluded that TiO 2 -based nano materials including titanate nanotubes had a strong dosedependent effect on cell proliferation and cell death (Magrez et al 2009). Rothen-Rutishauser et al (2007) reported that the size of nano-particles or their aggregates can be considered a potential determinant for uptake and subsequent macrophage responses that could explain for the observed differences in LDH release at the highest concentration of NPs (Albrecht et al 2009). Since particles in the fine size range may induce more pronounced responses than in the nanorange (Rothen-Rutishauser et al 2007), the ''ultrafine hypothesis'' has been challenged previously by experimental studies ).…”

Section: Discussionmentioning

confidence: 99%

Health risk assessments of lithium titanate nanoparticles in rat liver cell model for its safe applications in nanopharmacology and nanomedicine

et al. 2014

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Due to their high chemical stability, lithium titanate (Li2TiO3) nanoparticles (LTT NPs) now are projected to be transferred into different nanotechnology areas like nano pharmacology and nano medicine. With the increased applications of LTT NPs for numerous purposes, the concerns about their potential human toxicity effects and their environmental impact are also increased. However, toxicity data for LTT NPs related to human health are very limited. Therefore we aimed to investigate toxicity potentials of various concentrations (0-1,000 ppm) of LTT NPs (<100 nm) in cultured primary rat hepatocytes. Cell viability was detected by [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] (MTT) assay and lactate dehydrogenase (LDH) release, while total antioxidant capacity (TAC) and total oxidative stress (TOS) levels were determined to evaluate the oxidative injury. DNA damage was analyzed by scoring liver micronuclei rates and by determining 8-oxo-2-deoxyguanosine (8-OH-dG) levels. The results of MTT and LDH assays showed that higher concentrations of dispersed LTT NPs (500 and 1,000 ppm) decreased cell viability. Also, LTT NPs increased TOS (300, 500 and 1,000 ppm) levels and decreased TAC (300, 500 and 1,000 ppm) levels in cultured hepatocytes. The results of genotoxicity tests revealed that LTT NPs did not cause significant increases of micronucleated hepatocytes and 8-OH-dG as compared to control culture. In conclusion, the obtained results showed for the first time that LTT NPs had dose dependent effects on oxidative damage and cytotoxicity but not genotoxicity in cultured primary rat hepatocytes for the first time.

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Evaluation of cytotoxic effects and oxidative stress with hydroxyapatite dispersions of different physicochemical properties in rat NR8383 cells and primary macrophages

Cited by 45 publications

References 51 publications

The Risk Evaluation of Tungsten Oxide Nanoparticles in Cultured Rat Liver Cells for Its Safe Applications in Nanotechnology

The Risk Evaluation of Tungsten Oxide Nanoparticles in Cultured Rat Liver Cells for Its Safe Applications in Nanotechnology

Nanocrystalline silicon substituted hydroxyapatite effects on osteoclast differentiation and resorptive activity

Health risk assessments of lithium titanate nanoparticles in rat liver cell model for its safe applications in nanopharmacology and nanomedicine

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