Nanocrystalline silicon substituted hydroxyapatite effects on osteoclast differentiation and resorptive activity

Matesanz, María Concepción; Linares, Javier; Lilue, Isabel; Sánchez‐Salcedo, Sandra; Feito, María José; Arcos, Daniel; Vallet‐Regí, María; Portolés, María Teresa

doi:10.1039/c3tb21697g

Cited by 34 publications

(26 citation statements)

References 61 publications

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“…20 This effect has been also observed with cultured L929 fibroblasts, Saos-2 osteoblasts and MC3T3-E1 preosteoblasts. 14,21 On the other hand, nano-SiHA disks delayed the osteoclast differentiation and decreased the resorptive activity of these cells on their surface, as compared to nano-HA samples, without affecting cell viability, 22 thus indicating a beneficial action of Si-substituted material for bone regeneration in agreement with other studies. 13,15,17,23,24 Since the balance between proinflammatory (M1) and reparative (M2) macrophages has been involved in their negative or positive role in disease and tissue remodelling, 4,5,8,9 the effects of nano-HA and nano-SiHA on murine macrophage populations have been evaluated in the present study in basal conditions and in the presence of either pro-inflammatory (LPS) or anti-inflammatory (IL-10) stimuli.…”

Section: Introductionsupporting

confidence: 79%

Effects of nanocrystalline hydroxyapatites on macrophage polarization

et al. 2016

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The present study is focused on the effects of nanocrystalline hydroxyapatite (nano-HA) and nanocrystalline silicon substituted hydroxyapatite (nano-SiHA) on the macrophage populations defined as proinflammatory (M1) and reparative (M2) phenotypes. The results demonstrate that both nano-HA and nano-SiHA favour the macrophage polarization towards a M2 reparative phenotype, decreasing M1 population and ensuring an appropriate response in the implantation site of these biomaterials.We believe that this work will have an enormous interest for the use of these nanocrystalline hydroxyapatites for bone repair and bone tissue engineering. Silicon substituted and nanocrystalline hydroxyapatites have attracted the attention of many researchers due to their upregulation in osteoblast cell metabolism and enhanced bioreactivity, respectively. On the other hand, the biomaterial success or failure depends ultimately on the immune response triggered after its implantation. Macrophages are the main components of the innate immune system with an important role in healing and tissue remodelling due to their remarkable functional plasticity, existing a whole spectrum of functional populations with varying phenotypic features. The effects of nanocrystalline hydroxyapatite (nano-HA) and nanocrystalline silicon substituted hydroxyapatite (nanoSiHA) on the macrophage populations defined as proinflammatory (M1) and reparative (M2) phenotypes, have been evaluated in the present study with RAW 264.7 cells and mouse peritoneal macrophages as in vitro models. With the purpose of favouring the polarization of primary macrophages towards the M1 or M2 phenotype, the pro-inflammatory stimulus LPS or the anti-inflammatory stimulus IL-10 were used respectively, evaluating the biomaterial effects under these conditions. Our results show that both nano-HA and nano-SiHA favour the macrophage polarization towards a M2 reparative phenotype, decreasing M1 population and ensuring an appropriate response in the implantation site of these biomaterials designed for bone repair and bone tissue engineering.

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Section: Introductionsupporting

confidence: 79%

Effects of nanocrystalline hydroxyapatites on macrophage polarization

et al. 2016

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“…Because of their predominant role in the deposition of bone tissue, osteoblasts are commonly used for the in vitro evaluation of biomaterials. However, osteoclasts resorption is also essential for a complete bone regeneration and only few studies address the behavior of osteoclasts in contact with BAG …”

Section: Glassesmentioning

confidence: 99%

Optimized Bioactive Glass: the Quest for the Bony Graft

Granel

Bossard

Nucke

et al. 2019

Adv Healthcare Materials

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Technological advances have provided surgeons with a wide range of biomaterials. Yet improvements are still to be made, especially for large bone defect treatment. Biomaterial scaffolds represent a promising alternative to autologous bone grafts but in spite of the numerous studies carried out on this subject, no biomaterial scaffold is yet completely satisfying. Bioactive glass (BAG) presents many qualifying characteristics but they are brittle and their combination with a plastic polymer appears essential to overcome this drawback. Recent advances have allowed the synthesis of organic–inorganic hybrid scaffolds combining the osteogenic properties of BAG and the plastic characteristics of polymers. Such biomaterials can now be obtained at room temperature allowing organic doping of the glass/polymer network for a homogeneous delivery of the doping agent. Despite these new avenues, further studies are required to highlight the biological properties of these materials and particularly their behavior once implanted in vivo. This review focuses on BAG with a particular interest in their combination with polymers to form organic–inorganic hybrids for the design of innovative graft strategies.

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“…RAW‐264.7 cells were seeded on hydroxyapatite disks, prepared as previously described (Matesanz et al, ), placed into each well of a 24‐well culture plate, at a density of 1 × 10 4 cells/cm 2 in α‐MEM without phenol red and supplemented with 4% FBS. In order to stimulate osteoclast differentiation, 40 ng/ml of mouse RANKL and 25 ng/ml M‐CSF were added to the culture medium and cells were cultured for 14 days in the presence of MLO‐Y4 cell‐CM (20% in standard medium), renewing culture medium every 3 days, as previously reported (Torres‐Rodríguez et al, ).…”

Section: Methodsmentioning

confidence: 99%

High glucose alters the secretome of mechanically stimulated osteocyte‐like cells affecting osteoclast precursor recruitment and differentiation

Maycas

Portolés

Matesanz

et al. 2017

Journal Cellular Physiology

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Diabetes mellitus (DM) induces bone deterioration, while mechanical stimulation promotes osteocyte-driven bone formation. We aimed to evaluate the interaction of acute exposure (24 h) to high glucose (HG) with both the pro-survival effect conferred to osteocytic MLO-Y4 cells and osteoblastic MC3T3-E1 cells by mechanical stimulation and the interaction of these cells with osteoclast precursor RAW264.7 cells. We found that 24 h of HG (25 mM) pre-exposure prevented both cell survival and ERK and β-catenin nuclear translocation upon mechanical stimulation by fluid flow (FF) (10 min) in both MLO-Y4 and MC3T3-E1 cells. However, migration of RAW 264.7 cells was inhibited by MLO-Y4 cell-conditioned medium (CM), but not by MC3T3-E1 cell-CM, with HG or FF. This inhibitory effect was associated with consistent changes in VEGF, RANTES, MIP-1α, MIP-1β MCP-1, and GM-CSF in MLO-Y4 cell-CM. RAW264.7 proliferation was inhibited by MLO-Y4 CM under static or HG conditions, but it increased by FF-CM with or without HG. In addition, both FF and HG abrogated the capacity of RAW 264.7 cells to differentiate into osteoclasts, but in a different manner. Thus, HG-CM in static condition allowed formation of osteoclast-like cells, which were unable to resorb hydroxyapatite. In contrast, FF-CM prevented osteoclastogenesis even in HG condition. Moreover, HG did not affect basal RANKL or IL-6 secretion or their inhibition induced by FF in MLO-Y4 cells. In conclusion, this in vitro study demonstrates that HG exerts disparate effects on osteocyte mechanotransduction, and provides a novel mechanism by which DM disturbs skeletal metabolism through altered osteocyte-osteoclast communication.

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Nanocrystalline silicon substituted hydroxyapatite effects on osteoclast differentiation and resorptive activity

Cited by 34 publications

References 61 publications

Effects of nanocrystalline hydroxyapatites on macrophage polarization

Effects of nanocrystalline hydroxyapatites on macrophage polarization

Optimized Bioactive Glass: the Quest for the Bony Graft

High glucose alters the secretome of mechanically stimulated osteocyte‐like cells affecting osteoclast precursor recruitment and differentiation

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