Evaluation of Culture Media for In Vitro Conservation of Gladiolus Cultivars

Budiarto, Kurniawan; Rosario, Teresita L.

doi:10.17503/agrivita.v0i0.2314

Cited by 4 publications

(7 citation statements)

References 16 publications

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“…Silva et al [14] found that the regeneration of gladiolus from dormant cormlets suggested the fact regarding breaking of dormancy through hormonal action followed by consequent supply of readily available nutrients suitable to growing tissues from the medium. Induction of shoot bud within 7-8 days 16 and 7.8 days with maximum shooting (96%) [15] was observed in B5 (Gamborg) and MS medium respectively when it was supplemented with 1.0mg/L of BAP. Similar kind of findings was also reported by Budiarto [16] and [17].…”

Section: Resultsmentioning

confidence: 97%

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In-vitro Response of Gladiolus (var. American Beauty) towards Plant Growth Regulators Using Cormel Explants

Devi¹,

Maitra

Mandal

et al. 2023

IJECC

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Gladiolus is one of the most potential bulbous cut flower crops cultivated widely in India due to its attractive and long lasting spikes. The plant is propagated vegetatively through corms and cormels, but due to low rate of multiplication, its cultivation is hindered. Also, it is grown through underground modified stems, they are more often attacked by soil borne diseases such as Fusarium corm rot. In-vitro propagation techniques, assumes significance, especially for securing rapid multiplication of quality planting propagules using dormant cormlet explants and media. The results of the study revealed that the MS growing medium supplemented with 2 mg/l BAP showed improved results in respect of sprouting of explants (54.46 %), days required for sprouting of explants (8.68 DAI) and shoot length at 30 (6.32 cm), 60 (12.40 cm) and 90 (13.21 cm) DAI. The in vitro regenerated gladiolus plantlets when placed in the rooting MS medium supplemented with 4 mg/l IBA showed earlier root initiation (8.31 DAI) and higher number of roots/shoot (5.37 DAI) as compared to the rest of the treatment combinations. Hardening medium consisting of garden soil + sand + vermicompost (1:1:1) showed greater survivability (50.98 %) of plantlets. Thus, the study has been initiated an efficient protocol for in-vitro propagation of gladiolus through cormlets cultured in MS media containing BAP and IBA as shooting and rooting plant growth regulators and subsequent acclimatization in garden soil + sand+ vermicompost medium.

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Section: Resultsmentioning

confidence: 97%

“…Induction of shoot bud within 7-8 days 16 and 7.8 days with maximum shooting (96%) [15] was observed in B5 (Gamborg) and MS medium respectively when it was supplemented with 1.0mg/L of BAP. Similar kind of findings was also reported by Budiarto [16] and [17].…”

Section: Resultsmentioning

confidence: 97%

In-vitro Response of Gladiolus (var. American Beauty) towards Plant Growth Regulators Using Cormel Explants

Devi¹,

Maitra

Mandal

et al. 2023

IJECC

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“…Regarding the in vitro ability of three cultivars, Kaifa, Clara, and Nabila, observed maximum shoots and leaves for cv. Nabila at 2.0 mg L −1 BA, while the other two cultivars required higher concentrations [68]. Similarly, Memon et al [51] found that in comparison to other varieties, White Prosperity yielded more shoots on medium enriched with BAP (4 mg L −1 ); Traderhorn and Peter Pears, on the other hand, responded to shoot regeneration in statistically similar ways.…”

Section: Effect Of Plant Growth Regulatorsmentioning

confidence: 85%

“…Some key elements that affected plantlet in vitro efficiency are shown in Figure 2. Kaifa, Clara, and Nabila Direct organogenesis Nabila showed the best results in regard to the shoot development [68] Peter Pears, Rose Supreme, and White Prosperity Indirect organogenesis Rose Supreme induced a higher percentage of calluses [69] Figure 2. A schematic representation of some significant variables influencing the gladiolus's in vitro cormel formation and propagation.…”

Section: Indirect Organogenesismentioning

confidence: 99%

“…The genotype has a crucial impact on the achievement of any gladiolus in vitro development (Table 1). In a number of studies, the gladiolus genotype showed a considerable impact on callus induction [31,65,[68][69][70][71]. In the establishment stage of in vitro propagation of gladiolus with several genotypes, compact calluses formed in Blue Isle and Rosa supreme, while no callus was produced in Jenny Lee [65].…”

Section: Genotypes/species/cultivarsmentioning

confidence: 99%

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In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): A Systematic Review Analysis Based on More Than 50 Years Research

Kumar,

Chaudhary,

Sirohi

et al. 2024

Horticulturae

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Traditional gladiolus propagation methods are now supplemented with in vitro propagation to meet the demands of modern floriculture in terms of quick production of disease-free, quality planting material. Due to virus infections, vegetative propagation in gladiolus in the field is slow, and is a serious concern in the propagation of gladiolus. In vitro propagation provides an enormous increase in propagation rate and the ability to produce disease-free plant material. Numerous elements, including cultivars, explant type, size of explants, position of explants on medium, plant growth regulators and certain additives, incubation conditions, and sub-culturing time, all have a significant impact on in vitro clonal propagation of gladiolus plants as well as the development of in vitro cormel efficiency. There are certain obstacles and challenges that arise in the in vitro development of plants and the cormels of gladiolus. However, numerous studies and review reports on gladiolus for in vitro propagation have been reported, but very little is known about the factors influencing gladiolus’ in vitro effectiveness. In the present review, we focused on and analyzed research data accumulated over 50 years on diverse strategies for in vitro propagation such as direct, indirect organogenesis, and somatic embryogenesis, as well as various factors such as physical, nutritional, and hormonal influences on in vitro propagation, in vitro cormel formation efficiency, difficulties that arise, and new insights into in vitro development in gladiolus from the available literature worldwide. Future possibilities for further improvement in the in vitro propagation of ornamental gladiolus are also discussed. The current review provides insight into a comprehensive protocol for gladiolus in vitro propagation and emphasizes the importance of continuously advancing tissue culture techniques and factors influencing the in vitro efficiency towards improving in vitro plantlets and cormels in gladiolus (Gladiolus spp.).

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In Vitro Culture Technology and Advanced Biotechnology Tools Toward Improvement in Gladiolus (Gladiolus species): Present Scenario and Future Prospects

Kumar,

Sirohi,

Yadav

et al. 2023

Mol Biotechnol

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Evaluation of Culture Media for In Vitro Conservation of Gladiolus Cultivars

Cited by 4 publications

References 16 publications

In-vitro Response of Gladiolus (var. American Beauty) towards Plant Growth Regulators Using Cormel Explants

In-vitro Response of Gladiolus (var. American Beauty) towards Plant Growth Regulators Using Cormel Explants

In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): A Systematic Review Analysis Based on More Than 50 Years Research

In Vitro Culture Technology and Advanced Biotechnology Tools Toward Improvement in Gladiolus (Gladiolus species): Present Scenario and Future Prospects

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