Evaluation of cationic solid lipid microparticles as synthetic carriers for the targeted delivery of macromolecules to phagocytic antigen-presenting cells

The In vitro Sub-cellular Localization and In vivo Efficacy of Novel Chitosan/GMO Nanostructures containing Paclitaxel

“…Several physiochemical factors affecting cellular internalization of nanostructures have been identified including size and surface charge. Particles as large as 3 microns could be internalized by an order of magnitude larger than previously thought in phagocytic antigen-presenting cells (32,33). However, particles around 100 nm or smaller have been shown to have the most optimum rate of cellular uptake in epithelial cells and smooth muscle cells (34)(35)(36).…”

Solid lipid microparticles as an approach to drug delivery

“…Efficient internalisation of neutral and positively charged LMs by human primary macrophages was demonstrated in vitro by Erni et al [80]. Phagocytosis was followed by a rapid (24 h) intracellular particle degradation, making LM a suitable carrier for the immediate delivery of therapeutics to these cells.…”

“…For this in vitro approach, murine macrophage J774, human type lung alveolar (A549) and epithelial (Calu-3) cell lines have been used and no significant cytotoxic effects were observed in a wide range of particle concentrations [43,78,79], confirming the satisfactory tolerability of LMs. Erni et al [80] reported no cytotoxic effects for neutral LMs in different cell lines (293 embryonic kidney cells, murine macrophage cells), whereas cationic LMs displayed concentration-dependent cytotoxicity comparable to that of the cationic lipid. Moreover, a number of in vitro studies have demonstrated the low cytotoxicity of LNs on different cell lines [1,81] and these observations could be reasonably extrapolated to LMs.…”

Biological fate of food nanoemulsions and the nutrients they carry – internalisation, transport and cytotoxicity of edible nanoemulsions in Caco-2 intestinal cells

“…37 In contrast, cationic surfactants have been shown to have moderate-high toxicity, thought to arise from their greater ability to bind directly with cell membranes. 40,41 Despite widespread use of excipients (i.e. alcohol/polyol co-solvents) in microemulsion/nanoemulsion formulation there is limited study of the cytotoxicity of such a combination.…”