Evaluation of Antioxidant Properties, Phenolic Compounds, Anthelmintic, and Cytotoxic Activities of Various Extracts Isolated from <i>Nepeta cadmea</i>: An Endemic Plant for Turkey

Kaska, Arzu; Deniz, Nahide Gülşah; Çiçek, Mehmet; Mammadov, Ramazan

doi:10.1111/1750-3841.14167

Cited by 25 publications

(27 citation statements)

References 34 publications

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“…& Davis collected from Turkey possessed high amounts of chlorogenic and ferulic acids (63.52 and 14.65 mg/g extract) and exhibited strong antioxidant activity in DPPH free radical and ABTS cation radical scavenging, CUPRAC, β‐carotene bleaching, and phosphomolybdenum assays (Sarikurkcu et al, ). Among the ethanol, methanol, acetone, and water extracts prepared from N. cadmea Boiss., the water extract showed the highest DPPH free radical and ABTS cation radical scavenging and β‐carotene bleaching activities (Kaska, Deniz, Cicek, & Mammadov, ).…”

Section: Resultsmentioning

confidence: 99%

Phytochemical and biological investigations on twoNepetaspecies:Nepeta heliotropifoliaandN. congestasubsp.cryptantha

et al. 2019

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In the present study, the essential oil and aroma compositions of Nepeta heliotropifolia (NH) and N. congesta subsp. cryptantha (NC) were determined by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detector (GC/FID), and their phenolic compounds by liquid chromatography-tandem mass spectrometry (LC-MS/MS). In addition, antioxidant, cytotoxic, anticholinesterase, urease, and tyrosinase activities of essential oils obtained from NH and NC aerial parts and ethanol extracts prepared from different parts of NH and NC were investigated. The major constituent of water-distilled essential oils was found to be germacrene D (36.7% and 38.5%, respectively), and their main aroma component was eucalyptol (48.0% and 24.7%, respectively). Among the studied parts of NH and NC, their flowers extracts were found to be the richest in phenolic compounds and in which the most abundant compound was rosmarinic acid (8,909.91 and 4,317.20 μg/g, respectively). NH and NC flower extracts exhibited also strong antioxidant activity in DPPH, ABTS, and CUPRAC assays. Among the tested samples, NH essential oil indicated the best cytotoxic effect against PDF, HT-29, and MCF-7 (IC 50 52.34, 25.89, and 44.70 μg/ml, respectively), and the highest butyrylcholinesterase (77.21 ± 1.12% inhibition) and moderate acetylcholinesterase (41.36 ± 0.69% inhibition) inhibitory activities. Practical applicationsThis is the first report on the essential oil and aroma compositions, the phenolic compounds, the antioxidant with total phenolic and flavonoid contents, cytotoxic, anticholinesterase, urease, and tyrosinase activities of Nepeta heliotropifolia and N. congesta subsp. cryptantha, except for their essential oil compositions. The reported results suggested that Nepeta heliotropifolia and N. congesta subsp. cryptantha flowers being rich in rosmarinic acid and having strong antioxidant potential, and NH essential oil possessing significant cytotoxic and butyrylcholinesterase inhibitory effect could be source for nutraceutical, food, and drug industries. K E Y W O R D Saroma, biological activities, essential oil, LC-MS/MS, Nepeta, phenolic compound 2 of 12 | AKDENIZ Et Al.

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Section: Resultsmentioning

confidence: 99%

Phytochemical and biological investigations on twoNepetaspecies:Nepeta heliotropifoliaandN. congestasubsp.cryptantha

et al. 2019

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“…β-carotene/ linoleic acid bleaching assay was used to BHT and TROLOX as standards. These methods were described in our published paper (Kaska et al, 2018). The radical scavenging (DPPH and ABTS) and metal chelating activities were evaluated by the method described by Kaska et al, 2018, and these assays were used to EDTA, BHT and TROLOX as standards.…”

Section: Antioxidant Activitymentioning

confidence: 99%

The screening of Digitalis ferruginea L. subsp. ferruginea for toxic capacities, phenolic constituents, antioxidant properties, mineral elements and proximate analysis

et al. 2021

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The present study outlines the antioxidant and toxic activity, total phenol, flavonoid and tannin content of Digitalis ferruginea's extracts in addition to evaluating its proximate parameters and mineral elements. Successive extraction was made using different solvents (ethanol, acetone, water). Total phenol content was determined by the Folin-Ciocalteu's reagent method and flavonoid was determined by the aluminum chloride colorimetric method. The antioxidant activities were investigated using different assays. The phenolics were determined using HPLC. In addition an evaluation was made of the proximate parameters and mineral elements. The radical scavenging capacities were highest in the water extract. The same extract was effective in total antioxidant activities (β-carotene, 83.75%). The acetone extract demonstrated stronger reducing power and phosphomolybdenum antioxidant activity with 0.52 mg/mL and, 107.43 µg/mg, respectively. The HPLC results determined major phenolics: rutin and ferulic acid. This plant also have rich in polyphenolic content together with toxic activity and possesses nutrients and mineral elements. As well as demonstrating the good antioxidant activity of D. ferruginea this study suggest that the plant could be of particular interest from a practical perspective, as it is a significant source of potential natural antioxidants that can be used for the prevention of a range of diseases.

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“…The antioxidant capacity of O. sipyleum extract was also determined using the phosphomolybdenum method according to that of Prieto et al [18]. The sample preparation and procedure followed that given by Kaska et al [19]. The antioxidant activity of the extracts was calculated using the ascorbic acid graph.…”

Section: Determination Of Total Antioxidant Capacity By β-Carotene/limentioning

confidence: 99%

“…The total phenolic content was ascertained according to the Folin-Ciocalteu method [22], the total flavonoid content of the extracts was determined using the method of Arvouet-Grand et al [23] and the Tannin content was investigated using the vanillin-HCL method [24]. The method of Kaska et al [19] provided the sample preparation and procedure for determining the total phenolic, flavonoid and tannin content. The outcomes were shown as the equivalents of Gallic acid (mgGAE/g), quercetin (mgQEs/g) and catechin (mgCEs/g) for phenolic, flavonoid and tannin content respectively.…”

Section: Determination Of Total Phenolic Flavonoid and Tannin Contentsmentioning

confidence: 99%

“…The lethality of the ethanol extract from O. sipyleum was 327.414 µg/mL (LC50 < 1000 µg/mL), possessed high cytotoxic activities against brine shrimp and accepted as bioactive due to lower LC50. To date, various studies have been reported that Lamiaceae plants provide a rich source of phytochemical components which are considered to be the basis of various biological activities, including antioxidant, antibacterial, anthelmintic and cytotoxic activity [6,9,19,46,47]. Accordingly, it could be attributed that the presence of these active components are the basis of the cytotoxic activity.…”

Section: Cytotoxic Activitymentioning

confidence: 99%

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The phenolic content, antioxidant and cytotoxic activities of Origanum sipyleum from Turkey

Kaska

2018

International Journal of Secondary Metabolite

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Origanum sipyleum is a medicinal plant species belonging to the genus Origanum and traditionally vegetative parts of this plant have been used as medicinal tea or food additives. The current study has been designed to examine the antioxidant, cytotoxic activities and total phenolic, flavonoid and tannin contents as well as the chemical composition of the ethanol extract of O. sipyleum. The antioxidant capacity of O. sipyleum was determined using six complementary methods, β-carotene/linoleic acid and phosphomolybdenum test systems, radical scavenging (ABTS and DPPH), metal chelating and reducing power assays. The phenolics were identified using HPLC. A brine shrimp (Artemia salina L.) lethality test was used for determining cytotoxic activity. The ethanol extract exhibited high DPPH free radical scavenging (DPPH, IC50:102.75 µg/mL), ABTS radical scavenging (ABTS, IC50:88.64 µg/mL), metal chelating (20.68 %) and reducing power capacity (0.51 mg/mL). The antioxidant activities of the O. sipyleum with β-carotene/Linoleic acid and phosphomolybdenum were calculated as 85.59 % and 62.95 µg/mg respectively. The phenolic contents of the ethanol extract were evaluated using HPLC and determined major phenolics: caffeic acid, epicatechin and 2,5 dihydroxybenzoic. Furthermore, to gether with cytotoxic activity (LC50, 327.414 µg/mL) O. sipyleum is also rich in total phenolic, flavonoid and tannin contents were 203.57 ± 4.62 mgGAE/g, 46.98 ± 0.34 mgQE/g and 34.55 ± 0.56 mgCE/g respectively. These results could provide addition information for the potential use of this medicinal plant as a food ingredient and as a natural antioxidant in the diet, as well as for the pharmaceutical industry.

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Evaluation of Antioxidant Properties, Phenolic Compounds, Anthelmintic, and Cytotoxic Activities of Various Extracts Isolated from Nepeta cadmea: An Endemic Plant for Turkey

Cited by 25 publications

References 34 publications

Phytochemical and biological investigations on twoNepetaspecies:Nepeta heliotropifoliaandN. congestasubsp.cryptantha

Phytochemical and biological investigations on twoNepetaspecies:Nepeta heliotropifoliaandN. congestasubsp.cryptantha

The screening of Digitalis ferruginea L. subsp. ferruginea for toxic capacities, phenolic constituents, antioxidant properties, mineral elements and proximate analysis

The phenolic content, antioxidant and cytotoxic activities of Origanum sipyleum from Turkey

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