Evaluation of Antihyperglycemic Effect of Extract of Moringa stenopetala (Baker f.) Aqueous Leaves on Alloxan-Induced Diabetic Rats

Woldekidan, Samuel; Mulu, Abay; Ergetie, Wondwossen; Teka, Frehiwot; Meressa, Asfaw; Tadele, Ashenif; Abebe, Abiy; Gemechu, Worku; Gemeda, Negero; Ashebir, Rekik; Sileshi, Meron; Tolcha, Yoseph

doi:10.2147/dmso.s266794

Cited by 13 publications

(10 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A decrease in body weight is strongly linked with reduction in carbohydrate reserve as an instant source of energy and fats catabolism. In the current study, it was reported that oral administration of morin at dose range of 30–50 mg/kg body weight showed a marked increase in body weight that was in line with the previously reported studies [ 10 , 13 , 49 ] in a diabetic rat model.…”

Section: Discussionsupporting

confidence: 93%

Antioxidants Isolated from Elaeagnus umbellata (Thunb.) Protect against Bacterial Infections and Diabetes in Streptozotocin-Induced Diabetic Rat Model

et al. 2021

View full text Add to dashboard Cite

The increase in resistance of microbes against conventional drugs is currently a hot issue, whereas diabetes is another main cause of mortalities encountered throughout the world after cancer and heart attacks. New drug sources in the form of plants are investigated to get effective drugs for the mentioned diseases with minimum side effects. Elaeagnus umbellata Thunb. is a medicinal plant used for the management of stress related disorders like diabetes and other health complications. The active constituents of the chloroform extract derived from E. umbellata berries was isolated by silica gel column chromatography which were identified as morin, phloroglucinol, and 1-hexyl benzene through various spectroscopic techniques (electron ionization mass spectrometry, 1H-NMR, and 13C-NMR spectroscopy). The possible protective effects (antioxidant, antibacterial, and antidiabetic activity) of isolated compounds were evaluated using reported methods. Morin exhibited strong in vitro antiradical potential against DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid) radicals along with prominent antibacterial activities against selected bacterial strains (Escherichia coli, Bacillus cereus, Salmonella typhi, Klebsiella pneumonia, Pseudomonas aeruginosa and Proteus mirabilis). Among the isolated compounds the more potent one (morin) was assessed for its in vivo antidiabetic potential in streptozotocin-induced diabetic rat model. The in vivo effects observed were further confirmed in ex vivo experiments where the effect of isolated compound on antioxidant enzyme like glutathione peroxidase (GPx), total content of reduced glutathione (GSH), % DPPH inhibition, and the lipid peroxidation MDA (Malondialdehyde) level in pancreatic tissues homogenates were evaluated. In vivo morin at tested doses (2, 10, 15, 30 and 50 mg/kg body weight) significantly restored the alterations in the levels of fasting blood glucose level and body weight loss along with significant decrease in levels of cholesterol, triglycerides, low density lipoprotein, HbA1c level, and significantly increased the high-density lipoprotein in diabetic rats. Morin also effectively ameliorated the hepatic enzymes, and renal functions like serum creatinine. Morin significantly increased the antioxidant enzyme like GPx activity, GSH content, and % DPPH inhibition activity, while reduced the lipid peroxidation MDA (malondialdehyde) level in pancreatic tissues homogenates, and modification of histopathological changes in diabetic rats. Morin exhibited high antioxidant, antibacterial, and antidiabetic potentials as compared to phloroglucinol and 1-hexyl benzene, that could, therefore, be considered as a promising therapeutic agent to treat diabetes mellitus and bacterial infections.

show abstract

Section: Discussionsupporting

confidence: 93%

Antioxidants Isolated from Elaeagnus umbellata (Thunb.) Protect against Bacterial Infections and Diabetes in Streptozotocin-Induced Diabetic Rat Model

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Loading of sucrose (3 g/kg) was done for all groups after five minutes of administering the treatments. 14 Collection of blood samples was done from a tail vein at 0, 30, 60, 90, and 180 min. The glucose oxidase method was used to determine the blood glucose levels.…”

Section: Methodsmentioning

confidence: 99%

“…The crude aqueous and ethanol extract/fraction of the leaves have demonstrated hypoglycemic and antihyperglycemic activities. [12][13][14] Additionally, on chronic administration of the butanol fraction of Moringa stenopetala leaves antihyperglycemic and antihyperlipidemic effects were shown in alloxan-induced diabetic mice with wide margin of safety, indicating its potential for long-term management of diabetes and related complications including hyperlipidemia. 12,13,15,16 The antiglycation effect in in vitro studies also demonstrated the role of Moringa stenopetala in inhibiting diabetes-associated complications.…”

Section: Introductionmentioning

confidence: 99%

Antidiabetic Activity of Hot Tea Infusion of Leaves of Moringa stenopetala in Streptozotocin-Induced Diabetic Rats

Toma¹

2022

JEP

View full text Add to dashboard Cite

Background Moringa stenopetala is a traditionally used medicinal plant that has been used for the management of different disorders including diabetes in Ethiopia. This study was aimed to assess the antidiabetic activity of hot tea infusion of leaves of Moringa stenopetala in streptozotocin-induced diabetic rats. Methods Experimental animals were Wistar male rats aged 4–6 weeks weighing 200–250 gram. The animals were maintained in equal light/dark series of laboratory conditions, and the average ambient temperature was 23±2 °C. The hot tea infusion of Moringa stenopetala leaves of different concentrations (as fine and coarse powder decoction), distilled water (10 mL/kg), and 150 mg/kg body weight of metformin were administered to diabetic rats as test, negative control, and positive standard drugs, respectively. Sucrose oral challenge test was also carried out to assess the effect of Moringa stenopetala hot tea infusion on postprandial glucose level. Blood glucose level was measured at 0, 30, 60, 90, and 180 minutes. Statistical analysis was conducted by SPSS package version 23, and the p -value less than 0.05 was declared as level of statistical significance. Results The phytoconstituents that tested positive in hot tea infusion of Moringa stenopetala leaves were alkaloids, tannins, flavonoids, saponins, and terpenoids. Different experimental groups treated with the hot tea infusion of Moringa stenopetala leaves showed significant reduction of blood glucose level after 30 minutes of hot tea infusion administration. In addition, the hot tea infusion at a different concentration for both fine and coarse powders reduced significantly raised blood glucose level. The present findings revealed that the hot tea infusion has blood glucose-lowering and antioxidant effects with wide safety margin. Conclusion The findings indicated that the hot tea infusion of the leaves of Moringa stenopetala shows a credible reduction in blood glucose level in rats.

show abstract

“…Both enzymes have physiological and clinical significance. ALT levels in the liver have much higher concentrations than elsewhere, therefore increased ALT activity specifically reflects liver damage, which is a normal occurrence in diabetes possibly due to leakage of the enzyme into the bloodstream (Woldekidan et al, 2021). Various forms of cell death, both apoptosis and hepatocellular necrosis, have the potential to cause an increase in cell membrane permeability resulting in the release of transaminases in the bloodstream (Mallya et al, 2017;Omodanisi et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

The Effect Of Moringa Leaf Extract On Hyperglycemic Rat Liver Function

et al. 2022

View full text Add to dashboard Cite

One of the management of diabetics is to maintain stable glucose levels. Often diabetes treatment combines chemical drugs with medicinal plants. People have consumed a lot of Moringa leaves which are believed to be able to maintain body condition. One of the benefits of Moringa leaves with phytochemical components in it is as a hepatoprotector. The aim of this study was to analyze the role of Moringa leaf extract on liver function parameters of hyperglycemic rats. There were 4 groups, normal control (K0), hyperglycemic rats with induction of alloxan 125 mg/kg bw and divided into three groups treated with moringa leaf extract at a dose of 0 mg (K1), 200 mg (T1) and 400 mg/kg bw (T2 ) for 21 days. The variables measured were aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels, total cholesterol. Data were analyzed with Anova P0.05 and Least Significance Different (LSD) follow-up test. The results showed that the levels of AST and ALT were highest in the K1 group which then decreased significantly in the group that received Moringa extract (T1 and T2). Statistically there was a significant difference (p0.05) between the control and treatment groups. Cholesterol levels in the control group were significantly lower than the diabetes and treatment groups, but there was no significant difference between the treatment groups. The conclusion is that Moringa leaf extract has an effect on reducing liver enzyme levels and cholesterol in hyperglycemic rats. This research adds to the study of the hepatoprotective potential of Moringa leaf extract in hyperglycemic rats.

show abstract

Evaluation of Antihyperglycemic Effect of Extract of Moringa stenopetala (Baker f.) Aqueous Leaves on Alloxan-Induced Diabetic Rats

Cited by 13 publications

References 29 publications

Antioxidants Isolated from Elaeagnus umbellata (Thunb.) Protect against Bacterial Infections and Diabetes in Streptozotocin-Induced Diabetic Rat Model

Antioxidants Isolated from Elaeagnus umbellata (Thunb.) Protect against Bacterial Infections and Diabetes in Streptozotocin-Induced Diabetic Rat Model

Antidiabetic Activity of Hot Tea Infusion of Leaves of Moringa stenopetala in Streptozotocin-Induced Diabetic Rats

The Effect Of Moringa Leaf Extract On Hyperglycemic Rat Liver Function

Contact Info

Product

Resources

About