Evaluation of Antidiabetic and Antioxidant Effects of Seabuckthorn (Hippophae rhamnoides L.) in Streptozotocin-Nicotinamide Induced Diabetic Rats

Abstract

Seabuckthorn (Hippophae rhamnoides L.) is a branched deciduous shrub native to Europe and Asia. The plant has been used extensively in oriental traditional system of medicine for treatment of different diseases. Seabuckthorn is found to have significant antioxidative, antimicrobial, immunomodulatory, cytoprotective, hepatoprtective and tissue regenerative properties. The present study was undertaken to evaluate the antidiabetic and antioxidant effect of seabuckthorn (Hippophae rhamnoides L.) in streptozotocin-nicotinamide induced type-2 diabetic rats. Experimental diabetes was induced by a single intraperitonial injection of streptozotocin (60 mg/kg), 15 minutes after the i.p. administration of 120mg/kg nicotinamide. Seabuckthorn was administered orally to streptozotocin (STZ) diabetic rats. Blood glucose, tissue glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) in pancreas were estimated following the established procedures. Biochemical observations were supplemented with histological examination of pancreatic tissue. The increase of blood glucose, TBARS level with reduction in GSH content were the salient features observed in STZ-diabetic rats. Sea buckthorn produced a significant (p< 0.05) reduction in blood glucose levels and TBARS levels in the STZ-diabetic rats. GSH, reduced significantly (p< 0.05) in diabetic rats, was brought back to near normal levels by co-administration of sea buckthorn. Degenerative changes of pancreatic beta cells in STZ-diabetic rats were minimized to near normal morphology by administration of sea buckthorn as evident by histopathological examination. The results of the study indicate the role of oxidative stress in the induction of diabetes and suggest a protective effect of sea buckthorn in this animal model.

Effect of cocoyam (Colocasia esculenta), unripe plantain (Musa paradisiaca) or their combination on glycated hemoglobin, lipogenic enzymes, and lipid metabolism of streptozotocin-induced diabetic rats

“…Several authors have reported the ameliorating actions of different anti-diabetic plants on the HbA 1C status of streptozotocin diabetic rat models in short-term studies (Kumar et al, 2013;Sharma et al, 2011).…”

In vitro evaluation of antidiabetic and antioxidant activity of Seabuckthorn (Hippophae rhamnoides L.) leaves

“…India continues to be the 'Diabetic Capital' of the world with 50.8 million diabetics (Sharma et al, 2011). Similar trends have also been found in the companion animals, that is, dogs and cats.…”

“…Cao et al (2003) have also reported that flavonoids from the seed and fruit residue of Hippophae rhamnoides L. exhibited hypoglycaemia and hypolipidemic effects. Although there are reports of antidiabetic and antioxidant activity of seabuckthorn based on free radical scavenging activity, inhibition of lipid peroxidation and protection of -cells resulting in decreased blood glucose levels (Cao et al, 2003;Zhang et al, 2010;Pandurangan et al, 2011;Sharma et al, 2011), there is perhaps no previous study correlative on in vitro α-glucosidase inhibitory activity. Thus, the results obtained from the present study are useful in determining the mechanism of action of plant extracts.…”

Effect of livingstonepotato ( Plectranthus esculenthus N.E.Br) on hyperglycemia, antioxidant activity and lipid metabolism of streptozotocin induced diabetic rats

“…Several authors have reported the ameliorating actions of different anti-diabetic plants on the HbA 1C status of streptozotocin diabetic rat models in short term studies [29] , [30] , [31] .…”