In vitro evaluation of antidiabetic and antioxidant activity of Seabuckthorn (Hippophae rhamnoides L.) leaves

Abstract

Seabuckthorn (Hippophae rhamnoides L.) is thorny nitrogen fixing deciduous shrub native to Asia andEurope. The present study was undertaken to evaluate the antidiabetic and antioxidant effect of two different extracts, namely, methanolic and aqueous of seabuckthorn (H. rhamnoides L.) leaves by in vitro assays. Seabuckthorn leaves were procured from Lahul area of H.P. Leaves were dried and subjected to methanolic and aqueous extraction. Antidiabetic activity was measured by alphaglucosidase inhibitor assay. Antioxidant activity was studied by measuring the total antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity (DPPH) free radical scavenging assay and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) method. IC 50 values of both extracts were calculated for all the assays. The results showed that methanolic extract of seabuckthorn leaves have the potential to inhibit alpha glucosidase with IC 50 value of 0.25 mg/ml. Similar to antidiabetic effect, methanolic extract also exhibited better antioxidant activity as compared to aqueous extract. Therefore, it is suggested that methanolic extract of seabuckthorn leaves is a potential source for natural antidiabetic and antioxidant compounds and could have potential use in the management of diabetes mellitus.

Alpha amylase and Alpha glucosidase inhibitory effects of aqueous stem extract of Salacia oblonga and its GC-MS analysis

“…This is largely due to physical inactivity, unhealthy diets, obesity, raised blood cholesterol and glucose (Singh, Kumar, 2015). DM is a chronic disease, characterized by metabolic disorder associated with high blood sugar (Pallavi et al, 2015). It occurs due to the pancreas does not produce enough insulin or when the body cannot use the insulin effectively (Arumugam, Manjula, Paari, 2013).…”