EVALUASI ASPEK FARMASETIK DAN AKTIVITAS ANTIBAKTERI SECARA IN-VITRO KIT DIAGNOSTIK 99mTc-KANAMYCIN

Widyasari, Eva Maria; Sriyani, Maulana Eka; Halimah, Iim; Wongso, Hendris; Wibawa, Teguh Hafiz Ambar; Iswahyudi, Iswahyudi; Sidik, Ahmad

doi:10.17146/gnd.2015.18.1.2786

Cited by 2 publications

(2 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Infections treated include: bone, respiratory tract, skin, soft tissue, and abdominal infections, complicated urinary tract infections, endocarditis, septicaemia, and enterococcal infections (Roohi et al, 2006). Kanamycin has several electron Widyasari et al, 2015). Besides study of physico-chemical characteristics of 99m Tckanamycin have also been studied.…”

Section: Introductionmentioning

confidence: 99%

Biological Evaluation of 99mTc-Kanamycin for Infection Imaging

Widyasari

Halimah

Sugiharti

et al. 2017

ijpna

Self Cite

View full text Add to dashboard Cite

Kanamycin antibiotic was radiolabeled successfully with radioisotope technetium-99m for the potential use as radiopharmaceuticals for infection i maging. 99m Tc-kanamycin complexes was prepared 93 % radiochemical purities by direct labelling using 5 mg kanamycin and 30 µg SnCl2. The reaction occurred at alkaline condition (pH=9) and under room temperature for 30 min to achieve high radiochemical purity. Radiochemical purity and stability of 99m Tc-kanamycin was determined by ascending paper chromatography using Whatman 3 paper as the stationary phase, and acetone as the mobile phase to separate the radiochemical impurities in the form of 99m Tc-pertechnetate. While impurities in the form of 99m Tc-reduced were separated using the stationary phase ITLC-SG and 0.5 N NaOH as mobile phase. This study aimed to determine biological characteristic of 99m Tc-kanamycin radiopharmaceutical. In vitro cell studies showed that the change of kanamycin structure after labeling with technetium-99m did not give a specific influence to the potency of kanamycin as an antibiotic. In addition on uptake study, a significantly higher uptake of 99m Tc-kanamycin with S. aureus than E. coli. Biodistribution of 99m Tc-kanamycin complexes was studied on normal and infection mice at 15, 30, 60 and 120 min postinjections. The biodistribution of 99m Tc-kanamycin in infection mice showed that the complex accumulated in the infection sites. These results show that 99m Tc-Kanamycin radiopharmaceutical have a potential application for infection diagnosis.

show abstract

Section: Introductionmentioning

confidence: 99%

Biological Evaluation of 99mTc-Kanamycin for Infection Imaging

Widyasari

Halimah

Sugiharti

et al. 2017

ijpna

Self Cite

View full text Add to dashboard Cite

show abstract

“…More researches have been devoted to chemical modification of aminoglycoside antibiotics such as kanamycin with the goal of increasing antibacterial activities or new activities as antibacterial [30][31][32][33]. Tc-kanamycin labeling studies have been carried out in the previous research by the indirect method using pyrophosphate as a co-ligand with the result of labeling efficiency of above 95% [34][35][36]. However, the presence of radiochemical impurities in the form of Tc by the direct method.…”

mentioning

confidence: 99%

Preparation of <sup>99m</sup>Tc-Kanamycin Using a Direct Labeling Method

et al. 2016

View full text Add to dashboard Cite

Infectious diseases are still the leading cause of death in the world. The accurate technique for early detection and determination of the exact location of infection in the body is still needed. Nuclear techniques are capable for this purpose while other techniques such as MRI, USG and CT-SCAN sometimes cannot be applied. Tckanamycin radiopharmaceutical is complex of kanamycin and technetium-99m radionuclide, was used for bacterial detection of infection. The labeling studies of 99m Tc-kanamycin has been carried out by the indirect labeling method using pyrophosphate as a co-ligand with the results of labeling efficiency above 95%. However, the presence of radiochemical impurities in the form of 99m Tcpyrophosphate in the indirect labeling have to be considered which may interfere the imaging result. This study aimed to determine the optimum labeling conditions of Tc-kanamycin by direct labeling method. Kanamycin was successfully labeled with technetium-99m through direct labeling method. The labeling efficiency was determined by ascending paper chromatography using Whatman 3 paper as the stationary phase, and acetone as the mobile phase to separate the radiochemical impurities in the form of 99m Tc-pertechnetate. While impurities in the form of 99m Tcreduced were separated using the stationary phase ITLC-SG and 0.5 N NaOH as mobile phase. The experiment result showed that the optimum labeling conditions obtained by using 5 mg kanamycin, 30 µg SnCl 2 .2H 2 O, and pH of labeling was 9. The incubation time of labeling was 30 min at room temperature, provided labeling efficiency of 92.31 ± 1.74 %. The successful of kanamycin labeling with high efficiency makes 99m Tc-kanamycin can potentially be used as a radiopharmaceutical for the early detection of infectious diseases.

show abstract

EVALUASI ASPEK FARMASETIK DAN AKTIVITAS ANTIBAKTERI SECARA IN-VITRO KIT DIAGNOSTIK 99mTc-KANAMYCIN

Cited by 2 publications

References 3 publications

Biological Evaluation of 99mTc-Kanamycin for Infection Imaging

Biological Evaluation of 99mTc-Kanamycin for Infection Imaging

Preparation of <sup>99m</sup>Tc-Kanamycin Using a Direct Labeling Method

Contact Info

Product

Resources

About