Biological Evaluation of 99mTc-Kanamycin for Infection Imaging

Abstract

Kanamycin antibiotic was radiolabeled successfully with radioisotope technetium-99m for the potential use as radiopharmaceuticals for infection i maging. 99m Tc-kanamycin complexes was prepared 93 % radiochemical purities by direct labelling using 5 mg kanamycin and 30 µg SnCl2. The reaction occurred at alkaline condition (pH=9) and under room temperature for 30 min to achieve high radiochemical purity. Radiochemical purity and stability of 99m Tc-kanamycin was determined by ascending paper chromatography using Whatman 3 paper as the stationary phase, and acetone as the mobile phase to separate the radiochemical impurities in the form of 99m Tc-pertechnetate. While impurities in the form of 99m Tc-reduced were separated using the stationary phase ITLC-SG and 0.5 N NaOH as mobile phase. This study aimed to determine biological characteristic of 99m Tc-kanamycin radiopharmaceutical. In vitro cell studies showed that the change of kanamycin structure after labeling with technetium-99m did not give a specific influence to the potency of kanamycin as an antibiotic. In addition on uptake study, a significantly higher uptake of 99m Tc-kanamycin with S. aureus than E. coli. Biodistribution of 99m Tc-kanamycin complexes was studied on normal and infection mice at 15, 30, 60 and 120 min postinjections. The biodistribution of 99m Tc-kanamycin in infection mice showed that the complex accumulated in the infection sites. These results show that 99m Tc-Kanamycin radiopharmaceutical have a potential application for infection diagnosis.

Comparison of Antioxidant Activity of Mistletoe Mango Leaves Extract (Dendrophtoe pentandra (L.) Miq) Using Ethanol Solution and Methanol Solution Based on DPPH Method

“…Mistletoe mango (Dendrophthoe pentandra (L.) Miq) is widely distributed in China, Cambodia, India, Indonesia, Laos, Malaysia, Myanmar, Philippines, Thailand, and Vietnam [6]. In Indonesia, mistletoe mango is used to treat hypertension, cough, diabetes, cancer, diuretics, stomach ulcers, smallpox, skin infections, and after childbirth [7].…”

Technetium-99m-Labeled Diethylcarbamazine Citrate (^99mTc-DEC) as a New Diagnostic Agent for Lymphatic Filariasis Detection

“…The acute toxicity determination of 99m Tc-DEC was conducted in accordance with the methods and procedures in Indonesian Pharmacopoeia with a slight modification [19] by multiplying certain number of dose given to the test animals (Swiss mice) after converted into common dose received by humans. Each treatment was carried out on five mice.…”