Ethanolic Extract of Papaya (Carica papaya) Leaf Exhibits Estrogenic Effects In Vivo and In Silico

Abstract

The menopause women have the low level of estrogen in the body. The lack of estrogen changes physiological function in women’s body that affects in health condition. Carica papaya L. leaf contains flavonoid quercetin which exhibits estrogenic effect. The aim of this study is to determine the estrogenic effect of papaya leaves extract (PLE) in vivo, and in silico. Papaya leaves were extracted by ethanol 70% maceration. The in silico study were done by molecular docking between quersetin and Estrogen Receptor (ERα and ERβ) to obtain the docking score. Based on this study, docking score of quercetin was almost similar to the native ligand of ER. The in vivo study was done as follow: 36 female rats Sprague Dawley divided into six groups. The groups are shame-ovariectomized (S-OVX), control ovariectomized (OVX), CMC-Na control (OVX+CMC-Na), positive control (OVX+Estradiol), and the PLE treatment groups dose 750 mg/kgBW (OVX+750mg/kgBW) and dose 1000 mg/kgBW (OVX+1000 mg/kgBW). Administrations of PLE were done in three weeks orally, while estradiol was administrated intraperitonially. The mammae and uterine were sliced for analysis. Based on the study, the treatment of PLE increased the number of mammae lobules and uterine weight as well as estrogen does. In summary, PLE can be developed as a source of phytoestrogens.Keywords: Carica papaya L., phytoestrogen, estrogen receptor, mammae lobule, uterine

Effect of carica papaya leaf extract toward hatching and survival rate of nilem fish (Osteochilus hasselti) larvae

“…As much as 200 g of dried papaya leaf powder was extracted using maceration with 800 mL of 70% ethanol for 3 days at room temperature. The macerate was then filtered and the filtrate was dried using a rotary evaporator [16]. The papaya leaf extract then was screened to identify the phytochemical compounds that consisted of alkaloid, saponin, flavonoid, steroid, polyphenols, glycoside, tannin, quinone, and triterpenoid [17].…”

Uji Efektivitas Neuroprotektif Ekstrak Daun Pepaya (Carica papaya L.) Terhadap Sel Piramidal Di Hipokampus Dan Korteks Serebri Mencit (Mus musculus L.) Yang Diinduksi Trimetiltin

“…Ekstrak daun pepaya dosis 500 mg/kgBB (P1) dapat menurunkan persentase kerusakan sel piramidal secara nyata dibandingkan dengan kontrol positif. Hal ini diduga karena ekstrak daun pepaya selain mengandung myricetin (Ramezani et al, 2016) juga mengandung quercetin seperti yang dilaporkan oleh Sugiyanto et al (2012). Kandungan quercetin dalam daun pepaya diduga mampu meningkatkan kadar brain derived neurotrophic factor (BDNF) yang sangat penting perannya dalam pertumbuhan sel piramidal.…”