Uji Efektivitas Neuroprotektif Ekstrak Daun Pepaya (Carica papaya L.) Terhadap Sel Piramidal Di Hipokampus Dan Korteks Serebri Mencit (Mus musculus L.) Yang Diinduksi Trimetiltin

Masnunah, Siti; Wiratmini, Ngurah Intan; Suarni, Ni Made Rai

doi:10.24843/metamorfosa.2020.v07.i01.p05

Cited by 4 publications

(5 citation statements)

References 12 publications

(15 reference statements)

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“…If the data distribution was not normal, the Kruskal-Wallis test was used. If the results were found to be significantly different (p<0.05), the analysis proceeded with the Mann-Whitney test (Masnunah et al 2020).…”

Section: Methodsmentioning

confidence: 99%

Histological Study of Mice (Mus musculus Linn.) Digestive Organs Induced by Monosodium Glutamate after Consuming Roselle (Hibiscus sabdariffa Linn.) Tea

Sistra,

Wiratmini,

Sukmaningsih

2023

FMI

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Highlights: 1. This original study examined the antioxidants compounds derived from naturally sourced Hibiscus sabdariffa calyx 2. Hibiscus sabdariffa tea was able to maintain histological damage such as necrosis, degeneration cell, villous erosion, and epithelial desquamation of mice digestive organs after induced by MSG Abstract Monosodium glutamate (MSG) is one of the most frequently used synthetic additive for food flavoring ingredient. Excessive use of MSG can cause cytotoxic effects which impacted the levels of oxidative stress and free radicals in the body, especially in the human digestive system. Roselle (Hibiscus sabdariffa) is a plant that is known to has red flower petals, and has lots of beneficial active compounds such as polyphenols, flavonoids, anthocyanins, and other compounds which are antioxidants that function as free radical antidotes. This research aims to determine consumption effect of administration roselle tea, as well as its optimal dose for digestive organs restoration on MSG-induced mice. This research applied the completely randomized method, using 25 mice. Mice were divided into 5 treatments, consisting of K- (0.3 ml aquades), K+ (given 4 mg/g bw MSG), P1 (given 4 mg/g bw MSG and 2.6 mg/g bw Roselle), P2 (given 4 mg/g bw MSG and 3.9 mg/g bw Roselle), P3 (given 4 mg/g bw MSG and 5.2 mg/g bw Roselle). Treatment was administered orally using a gavage for 30 days. Histopathological examination of the stomach, duodenum, and liver was made by using the paraffin method and Hematoxylin-Eosin (HE) staining. Parameters observed in the stomach and duodenum were necrosis, inflammatory cell infiltration, villous erosion and epithelial desquamation, while in the hepatic organ parameters observed were necrosis, inflammatory cell infiltration and cell degeneration. The results of the parameters were statistically analyzed using the SPSS for windows version 22 application program. Normal and homogeneous data distribution was analyzed using the Kolmogorov-Smirnov normality test, analyzed by One-Way ANOVA test, followed by Duncan Post-Hoc test if the data were normally distributed and there were significant differences (P < 0.05) between groups. In conclusion, the infusion of roselle tea (H. sabdariffa) with graded doses were able to recover histological damage in the stomach, duodenum, and liver of mice (Mus musculus) that had been induced by MSG.

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Section: Methodsmentioning

confidence: 99%

Histological Study of Mice (Mus musculus Linn.) Digestive Organs Induced by Monosodium Glutamate after Consuming Roselle (Hibiscus sabdariffa Linn.) Tea

Sistra,

Wiratmini,

Sukmaningsih

2023

FMI

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“…41 hingga didapatkan filtrat. Filtrat kemudian dilakukan evaporasi menggunakan rotary evaporator (Heidolph, Schwabach, Germany) suhu 40 o C sehingga didapatkan ekstrak etanol biji teratai [12]. Rendemen yang diperoleh dihitung persentase bobot (b/b) berdasarkan standar Farmakope Herbal Indonesia, dengan menggunakan persamaan berikut [13].…”

Section: Ekstraksi Biji Teratai (Nymphaea Pubescens Willd)unclassified

“…Asam oleanolat berperan sebagai anti inflamasi yang dapat menurunkan tingkat ekspresi TNF-α, IL-1β dan IL-6 pada sel BV2 [11]. Selain itu, senyawa flavonoid berperan sebagai antioksidan yang dapat meningkatkan vaskularisasi perifer dan juga meningkatkan aliran darah serebral dalam mencegah neurodegenerasi [12]. Dalam penelitian ini, kami mengembangkan model penyakit neurodegeneratif menggunakan TMT sebagai agen neurotoksik untuk menginduksi penyakit neurodegeneratif.…”

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Efektivitas Ekstrak Biji Teratai (Nymphaea pubescens Willd) dalam Meningkatkan Perilaku Neurokognitif pada Mencit yang Diinduksi Trimetiltin

2023

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Neurodegenerasi adalah penyakit yang mengakibatkan degenerasi progresif dan kematian sel saraf di otak. Penelitian ini bertujuan untuk mengungkap khasiat neuroprotektif ekstrak biji teratai (Nymphaea pubescens Willd) melalui analisis perilaku neurokognitif. Sebanyak 25 ekor mencit jantan dibagi secara acak menjadi 5 perlakuan: kontrol negatif (Na-CMC 0,5%), kontrol positif diinduksi trimetiltin (TMT) 0,6mg/kgBB, dan pemberian ekstrak biji teratai (100, 200, dan 400 mg/kgBB). Induksi degenerasi otak dengan TMT menggunakan dosis tunggal secara intraperitoneal. Ekstrak diberikan tiga hari pasca injeksi TMT secara oral setiap hari selama 28 hari. Pada akhir perlakuan, tes neurobehavioral dilakukan meliputi uji kecerdasan memori dengan Hebb-Williams Maze, uji keingintahuan dengan Hole-Board dan uji interaksi sosial. Selanjutnya dilakukan pengukuran indeks organ otak. Data dianalisis secara statistik dengan One-Way Anova dilanjutkan dengan uji DNMRT (P<0,05). Hasil penelitian menunjukkan bahwa ekstrak biji teratai dosis 200 dan 400 mg/kgBB secara signifikan dapat meningkatkan kecerdasan kognitif (memori, keingintahuan dan interaksi sosial) pada mencit yang diinduksi TMT, namun tidak berpengaruh signifikan terhadap indeks bobot otak. Temuan penelitian ini mengungkapkan bahwa biji teratai adalah kandidat kuat untuk sumber obat anti-neurodegeneratif.

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“…The CMC and TMT were injected intraperitoneally (i.p). The dose of TMT (0.6 mg/ kg BW; Trimethyltin chloride, Sigma-Aldrich, Inc., St. Louis, MO) was determined based on a previous study (12). The oral gavages of NPE were started three days after TMT injection and performed continuously once a day at 09.00 am for 28 days.…”

Section: Animal Model and Experimental Designmentioning

confidence: 99%

Neuroprotective effect of water lily (Nymphaea pubescens Willd) seed ethanolic extract against trimethyltin-induced cognitive impairment and neurodegeneration in mice

Belahusna,

Santoso,

Rahayu

2023

J Herbmed Pharmacol

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Introduction: Cognitive impairments are profound outcomes of neurodegenerative disease, a global health issue. Water lily (Nymphaea pubescens, Nymphaeaceae) extracts have been reported to counteract oxidative stress. However, their protective effects against neurodegenerative disease remain to be fully investigated. The current study aimed to determine the neuroprotective effect of water lily seed ethanolic extract on trimethyltin (TMT)-induced cognitive impairment and neurodegeneration in a mouse model. Methods: A single dose of TMT (0.6 mg/kg BW) was intraperitoneally injected to young adult male mice followed by daily oral treatments with different doses of water lily seed extract (0, 100, 200, and 400 mg/kg BW) for 28 days. Thereafter, cognitive behaviors were assessed, malondialdehyde (MDA) levels and catalase (CAT) activities were determined, followed by histopathological examination of the brain. Results: The results revealed that, compared to the non-treated group, the water lily extract at doses of 100–400 mg/kg BW was effective in counteracting the decline in memory and spatial cognition of TMT-induced impairment (P<0.05). Moreover, the extract, particularly at doses of 200 and 400 mg/kg BW, substantially lowered the MDA level while elevating the CAT activity level (P<0.05). Water lily seed extract also significantly reduced TMT-induced pyramidal cell degeneration in the hippocampus and cerebral cortex (P<0.05). Conclusion: Our findings demonstrated that ethanolic extract from water lily seeds could effectively reduce TMT-induced cognitive impairment and MDA levels and enhance CAT activity thereby precluding neurodegeneration in the hippocampus and cerebral cortex. Thus, water lily seed extract is a potent candidate for a natural anti-neurodegenerative supplement.

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Uji Efektivitas Neuroprotektif Ekstrak Daun Pepaya (Carica papaya L.) Terhadap Sel Piramidal Di Hipokampus Dan Korteks Serebri Mencit (Mus musculus L.) Yang Diinduksi Trimetiltin

Cited by 4 publications

References 12 publications

Histological Study of Mice (Mus musculus Linn.) Digestive Organs Induced by Monosodium Glutamate after Consuming Roselle (Hibiscus sabdariffa Linn.) Tea

Histological Study of Mice (Mus musculus Linn.) Digestive Organs Induced by Monosodium Glutamate after Consuming Roselle (Hibiscus sabdariffa Linn.) Tea

Efektivitas Ekstrak Biji Teratai (Nymphaea pubescens Willd) dalam Meningkatkan Perilaku Neurokognitif pada Mencit yang Diinduksi Trimetiltin

Neuroprotective effect of water lily (Nymphaea pubescens Willd) seed ethanolic extract against trimethyltin-induced cognitive impairment and neurodegeneration in mice

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