Estudo Anatomopatológico Em Tecidos Condenados Pelo Serviço De Inspeção Federal (Sif) Por Suspeita De Tuberculose

Mendes, Ricardo E.; Schneider, Alessandro Vinícios; Werlich, Dennys Eduardo; Lucca, Neuber J.; Lorenzett, Marina Paula; Pilati, Célso

doi:10.5216/cab.v14i4.8581

Cited by 6 publications

(5 citation statements)

References 20 publications

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“…It is worth mentioning that some microorganisms besides these, such as Actinomyces spp. and Actinobacillus spp., are also responsible for causing granulomatous lesions similar to tuberculosis lesions (21).…”

Section: Discussionmentioning

confidence: 99%

Genotypic Characterization of Mycobacterium bovis Isolates From Dairy Cattle Diagnosed With Clinical Tuberculosis

et al. 2021

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Molecular diagnosis of bovine tuberculosis plays an essential role in the epidemiological knowledge of the disease. Bovine tuberculosis caused by Mycobacterium bovis represents a risk to human health. This study aimed to perform the genotypic characterization of M. bovis isolated from bovines diagnosed as tuberculosis from dairy herds in the state of Pernambuco, Brazil. Granulomas from 30 bovines were sent for microbiological culture, and colonies compatible with Mycobacterium spp. were obtained in at least one culture from 17/30 granulomas. All isolates were confirmed to be M. bovis by spoligotyping and 24loci MIRU-VNTR typing. While spoligotyping characterized the isolates as SB0121, SB0295, SB0852, SB0120, and an unclassified genotype, 24loci MIRU-VNTR rendered two clusters of two isolates each and 13 unique profiles. Loci ETR-A showed higher discriminatory power, and loci (ETR-B, ETR-C, MIRU16, MIRU27, and QUB26) showed moderate allelic diversity. This is the first study on the genetic variability of the infectious agent cause of bovine TB in Pernambuco and demonstrates variability of strains in the state. Thus, it corroborates the importance of this microorganism as agent of bovine tuberculosis and its zoonotic potential, this epidemiological tool being a determinant in the rigor of the sanitary practices of disease control in dairy herds.

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Section: Discussionmentioning

confidence: 99%

Genotypic Characterization of Mycobacterium bovis Isolates From Dairy Cattle Diagnosed With Clinical Tuberculosis

et al. 2021

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“…Multiple illnesses can have the same macroscopic features, which can increase the misdiagnosis probability occurring at the time of macroscopic inspection. Thus, complementary tests can be performed, aiming at confirming the presumptive diagnosis (Mendes et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Eurythrematosis as a developmental model of the Diabetes Mellitus type 1 pathological condition: pathophysiological parameters and oxidative stress / Eurytrematose como modelo de desenvolvimento da patologia da Diabetes Mellitus tipo 1: parâmetros fisiopatológicos e stress oxidativo

Rosa¹,

Nodari²,

Suthovski³

et al. 2021

BJDV

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Eurytrematosis is a helminthic disease caused by trematodes belonging to the genus Eurytrema spp. that parasitize the pancreas of many animals and humans. This parasitosis causes chronic fibrosing pancreatitis, fat infiltration in the pancreatic parenchyma, besides damaging the exocrine pancreas, which is similar to that found in patients with Diabetes Mellitus type 1 (DM1). The current work aimed to evaluate the use of bovine pancreas infected with E. coelomaticum as a model to study DM1 pathophysiology. It was carried out macroscopic analyses, parasite identification, total pancreatic lipid determination and oxidative damage biomarkers levels of pancreas naturally infected with E. coelomaticum. Macroscopically, we observed that the infected pancreas had duct obstruction, organ stiffness due to the visible presence of fibrosis, increased adipose tissue deposition, increased protein and lipid damage, as well as increased antioxidant biomarkers (GSH, CAT and VIT C). Thus, it is possible to show that DM1 may have pancreatic parasitism as a possible primary origin. However, more studies are needed to better investigate this possible primary origin; the results obtained here suggest that the use of pancreas parasitized by E. coelomaticum could be a model to investigate DM1 pathophysiology.

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“…Low rates of mycobacterial isolation have been reported in previous studies addressing lesions suggestive of BTB (Pinto et al 2002, Araújo et al 2005. It can also be explained by the occurrence of nodular and granulomatous inflammatory processes determined by other etiologies, with specific characteristics, and undifferentiated gross features of BTB, such as lymphosarcoma, actinomycosis, actinobacillosis, and nocardiosis (Roxo 1997, Mendes et al 2013, Paes & Franco 2016. The absence or deficiency of viable pathogens in suggestive gross lesions may also be associated with the difficulty of the pathogen to multiply in culture, because the lesions present a bacillary concentration below 10,000 CFU/mL (Zanini et al 2001), and the death of mycobacteria after promoting the lesion by defending the organism from the chronic nature of the disease (Pinto et al 2002, Fráguas et al 2008.…”

Section: Discussionmentioning

confidence: 99%

“…It is known that results obtained from surveillance in slaughterhouses can indicate BT health status in the herd. However, due to the similarity between the disease's lesions and other nodular and granulomatous inflammatory processes, it is often impossible to identify all sick animals (Roxo 1997, Mendes et al 2013. In general, recognition of gross lesions associated with BT during routine health inspection in slaughterhouses has been the only measure used to diagnose the disease, which may incur errors in its detection, especially in paucibacillary lesions.…”

Section: Introductionmentioning

confidence: 99%

Isolation and genotyping of Mycobacterium bovis in suggestive lesions of tuberculosis in cattle slaughtered in the state of Ceará, Brazil

et al. 2020

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Bovine tuberculosis (BTB) is a zoonosis caused by the bacterium Mycobacterium bovis, which induces the development of nodular and granulomatous lesions in various animal tissues. The recognition of these suggestive gross lesions during postmortem sanitary inspection in slaughterhouses provides a presumptive diagnosis, which requires the use of complementary tests to confirm the disease. This study aimed to verify the occurrence of BTB in cattle slaughtered in slaughterhouses in the state of Ceará, Brazil, using bacteriological and molecular methods. To this end, suggestive lesions were analyzed on carcasses condemned by the “Serviço de Inspeção Estadual” (SIE). The samples were submitted to microbiological analysis using culture media and specific staining followed by spoligotyping molecular technique for identification and genotyping of the mycobacteria. Occurrence of lesions suggestive of BTB was verified in bovine carcasses (0.071%) from different municipalities of the state. These lesions were located mainly in the lung (95.12%), lymph nodes (58.53%), and liver (36.58%). Microbiological culture showed bacterial isolation (17.94%), with the growth of colonies showing morphological and tannic characteristics belonging to genus Mycobacterium spp. Genetic polymorphism analysis identified M. bovis in all isolates, which were discriminated into six spoligotypes (SB0121, SB0295, SB1064, SB0120, SB0870, and SB0852). These profiles have been described in Brazil and several areas of the world, except for profiles SB1064 and SB0852, which were described in the country for the first time. The results show that the association of the diagnostic methods used was the basis for the first study on identification of mycobacteria found in the state, which may provide a database for the epidemiological study of BTB in the state of Ceará.

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Estudo Anatomopatológico Em Tecidos Condenados Pelo Serviço De Inspeção Federal (Sif) Por Suspeita De Tuberculose

Cited by 6 publications

References 20 publications

Genotypic Characterization of Mycobacterium bovis Isolates From Dairy Cattle Diagnosed With Clinical Tuberculosis

Genotypic Characterization of Mycobacterium bovis Isolates From Dairy Cattle Diagnosed With Clinical Tuberculosis

Eurythrematosis as a developmental model of the Diabetes Mellitus type 1 pathological condition: pathophysiological parameters and oxidative stress / Eurytrematose como modelo de desenvolvimento da patologia da Diabetes Mellitus tipo 1: parâmetros fisiopatológicos e stress oxidativo

Isolation and genotyping of Mycobacterium bovis in suggestive lesions of tuberculosis in cattle slaughtered in the state of Ceará, Brazil

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