Genotypic Characterization of Mycobacterium bovis Isolates From Dairy Cattle Diagnosed With Clinical Tuberculosis

Melo, Elizabeth Hortêncio de; Gomes, Harrison Magdinier; Suffys, Philip Noël; Lopes, Mariana Canuto Melo de Sousa; Teixeira, Raquel Lima de Figueiredo; Santos, Ícaro Rodrigues dos; Franco, Marília Masello Junqueira; Langoni, Hélio; Paes, Antônio Carlos; Afonso, José Augusto Bastos; Mendonça, Carla Lopes de

doi:10.3389/fvets.2021.747226

Cited by 3 publications

(1 citation statement)

References 28 publications

(51 reference statements)

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“…A similar phenomenon was noted in this present study. A recent study by Melo and colleagues 28 carried out in Brazil also noted such a phenomenon and attributed it to possible degradation of the genetic material or mutations in the primer binding region of the specific loci. Also, Ei and colleagues postulated that the phenomenon could be as a result of deletions in the region.…”

Section: Discussionmentioning

confidence: 90%

Genetic Diversity of Mycobacterium tuberculosis complex isolates circulating in North Central Nigeria

Makpu,

Otokunefor,

Otokunefor

2023

Proceedings of the 6th Biennial Conference of Organization for Women in Science for the Developing World Nigeria (OWSD Garden C

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With the higher risk of tuberculosis associated with developing countries, it has increasingly become imperative to determine the genetic diversity of strains of the Mycobacterium tuberculosis complex (MTBC). This is essential for establishing control programs as it provides information on possible sources and chains of transmission.This study aimed to assess the genetic diversity of M. tuberculosis complex strains isolated from the North Central Nigeria using the 12-locus MIRU-VNTR.Mycobacterium tuberculosis complex isolates (106) obtained from a previous study between November 2017 and December 2018 were characterized using the 12-locus mycobacterial interspersed repetitive unit variable number tandem repeats (MIRU-VNTR) typing method. Data generated were then analyzed using the MIRU-VNTRplus web application to determine the allelic diversity.Allelic diversity as determined by the Hunter-Gaston diversity index showed a variation from 0.21 to 0.81 associated with MIRU locus 2 and 26 respectively. In total, 41.7% of the loci were highly discriminant, 41.7% moderately discriminant, and 16.7% were poorly discriminant. MIRU-VNTR typing revealed a high diversity, with a clustering rate of 1.9% and a total of ten different genotypes. UgandaII (Lineage 4) was the most common (14, 21.2%), followed by Bovis (10, 15.1%) and Cameroon (6, 9.1%); UgandaI (Lineage 4) was the least common (1, 1.5%).

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Section: Discussionmentioning

confidence: 90%

Genetic Diversity of Mycobacterium tuberculosis complex isolates circulating in North Central Nigeria

Makpu,

Otokunefor,

Otokunefor

2023

Proceedings of the 6th Biennial Conference of Organization for Women in Science for the Developing World Nigeria (OWSD Garden C

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First Insight into Diversity of Minisatellite Loci in Mycobacterium bovis/M. caprae in Bulgaria

Terentieva

Savova-Lalkovska²,

Dimitrova³

et al. 2023

Diagnostics

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The aim of this study was to assess the diversity of minisatellite VNTR loci in Mycobacterium bovis/M. caprae isolates in Bulgaria and view their position within global M. bovis diversity. Forty-three M. bovis/M. caprae isolates from cattle in different farms in Bulgaria were collected in 2015–2021 and typed in 13 VNTR loci. The M. bovis and M. caprae branches were clearly separated on the VNTR phylogenetic tree. The larger and more geographically dispersed M. caprae group was more diverse than M. bovis group was (HGI 0.67 vs. 0.60). Overall, six clusters were identified (from 2 to 19 isolates) and nine orphans (all loci-based HGI 0.79). Locus QUB3232 was the most discriminatory one (HGI 0.64). MIRU4 and MIRU40 were monomorphic, and MIRU26 was almost monomorphic. Four loci (ETRA, ETRB, Mtub21, and MIRU16) discriminated only between M. bovis and M. caprae. The comparison with published VNTR datasets from 11 countries showed both overall heterogeneity between the settings and predominantly local evolution of the clonal complexes. To conclude, six loci may be recommended for primary genotyping of M. bovis/M. caprae isolates in Bulgaria: ETRC, QUB11b, QUB11a, QUB26, QUB3232, and MIRU10 (HGI 0.77). VNTR typing based on a limited number of loci appears to be useful for primary bTB surveillance.

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Epidemiological Analysis of Human and Animal Originated Mycobacterium bovis Strains by Spoligotyping and MIRU-VNTR Methods

Altun,

Pir,

Yardımcı

2024

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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This study aims to investigate the genotypic similarities between human and animal-originated isolates by spoligotyping and 24 loci MIRU-VNTR for molecular epidemiological analysis of M. bovis isolates. In this study, isolates were obtained between 2019-2022 from 58 humans and 50 bovines. All isolates were initially identified with the GenoType MTBC kit and genotyped with spoligotyping and 24 loci MIRU-VNTR methods and their lineage relationships were shown in the dendrogram. When the human and animal-originated isolates were tested by the spoligotyping method, eight different clusters and 29 different genotypes were observed. Among these genotypes, the most common ones were found to be SIT1118/SB0989 (19.23%), SIT482/SB0120 (16.35%), SIT685/SB0288 (12.5%) and they were detected in isolates of both human and animal-originated. SB1593 (12.5%) was detected only in isolates of animal-originated. Other genotypes were found as SIT3529/SB0920, SIT1185/SB0897, SIT3710/SB1595, SIT688/SB0129, SIT3687/SB1625, SB0419, SB2466, SB1231, and SB2510. Nine different clusters and 55 different genotypes were obtained with MIRU-VNTR. ETR-C, QUB2163b, QUB26, and Mtub04 had the most allelic diversity. It was observed that MIRU02, MIRU20, MIRU24, MIRU27, and MIRU39 did not indicate allelic diversity. In conclusion, it was seen that the spoligotyping was easier to implement and evaluate compared to MIRU-VNTR. It was concluded that both tests can be used safely. Similar genotypes in humans and animals indicate the importance of zoonotic transmission of bovine tuberculosis.

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Genotypic Characterization of Mycobacterium bovis Isolates From Dairy Cattle Diagnosed With Clinical Tuberculosis

Cited by 3 publications

References 28 publications

Genetic Diversity of Mycobacterium tuberculosis complex isolates circulating in North Central Nigeria

Genetic Diversity of Mycobacterium tuberculosis complex isolates circulating in North Central Nigeria

First Insight into Diversity of Minisatellite Loci in Mycobacterium bovis/M. caprae in Bulgaria

Epidemiological Analysis of Human and Animal Originated Mycobacterium bovis Strains by Spoligotyping and MIRU-VNTR Methods

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