Establishment of an in vitro micropropagation protocol for Mecardonia tenella

Alderete, Liliana Marisol; Mori, Marcela; Kato, Adriana; Escandón, Alejandro Salvio

doi:10.2225/vol9-issue3-fulltext-6

Cited by 7 publications

(5 citation statements)

References 3 publications

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“…But unlike the latter, the formulations with relatively lower concentrations resulted in poorly developed multiple shoots, which were pale green and lanky in appearance. This may be due to the minimum levels of exogenous PGRs required to balance with endogenous plant hormones for retaining the plant health (Alderete et al 2006), which was not present in the formulations with relatively lower concentrations. The addition of NAA along with BAP for multiple shoot proliferation proved to be detrimental as it encouraged undesirable root formation under multiple shoots.…”

Section: Discussionmentioning

confidence: 99%

An elite protocol for accelerated quality-cloning in Gerbera jamesonii Bolus cv. Sciella

Gantait

Mandal

Bhattacharyya

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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A novel, efficient, and simple protocol was developed on in vitro mass propagation and acclimatization of Gerbera jamesonii Bolus cv. Sciella, an ornamental plant with attractive flowers. Shoot tip was used as the primary explant for in vitro establishment in which Murashige and Skoog (MS) medium supplemented with a low level of NAA (0.5 mgl −1 ) and BAP (1.5 mgl −1 ) promoted earliest axillary bud initiation within 5 d in 91.6% of the inoculants. Five axillary buds were initiated from a single explant within 13 d after inoculation. A very high rate of shoot multiplication (14 shoots per inoculated axillary bud) and proliferation was achieved when MS medium was fortified with a relatively higher level of BAP (2 mgl −1 ) and 60 mgl −1 ADS within 27 d of multiple shoot culture. A maximum number of well-developed roots per plant was observed in MS medium with 0.5 mg l −1 IAA in the next 26 d. In the easy low-cost acclimatization process of 20 d, a combination of sand, soil, cow urine, and tea leaves extract (1:1:1:1; v/v) ensured 95% survival rate. Sixty-one well-acclimatized plants were obtained from a single shoot tip within 86 d. The sustained multiple shoot culture for 15 mo paved the way toward the conservation of genetic resources as well as beneficial economics. The clonal fidelity study of micropropagated and sustained cultured clones using ISSR primers ensured the continuous supply of quality propagules retaining genetic uniformity. The in vitro-generated plants performed better over conventionally propagated plants in the field condition.

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Section: Discussionmentioning

confidence: 99%

An elite protocol for accelerated quality-cloning in Gerbera jamesonii Bolus cv. Sciella

Gantait

Mandal

Bhattacharyya

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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“…Nodal segments (length: 0.5 cm) of four different plants ( Bacopa monnieri, Eupatorium triplinerve, Excoecaria agallocha , and Avicennia marina ) were disinfected by sonicating in water for 20 min and dipping in 70% ethanol for 1 min, followed by 25 min of sodium hypochlorite (25%)/Tween 80 (0.01%) solution and rinsed three times with distilled and sterile water [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

Plant Growth Promoting of EndophyticSporosarcina aquimarinaSjAM16103 Isolated from the Pneumatophores ofAvicennia marina L.

Janarthine

Eganathan

2012

International Journal of Microbiology

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EndophyticSporosarcina aquimarinaSjAM16103 was isolated from the inner tissues of pneumatophores of mangrove plantAvicennia marinaalong withBacillussp. andEnterobactersp. EndophyticS. aquimarinaSjAM16103 was Gram variable, and motile bacterium measured 0.6–0.9 μm wide by 1.7–2.0 μm long and light orange-brown coloured in 3-day cultures on tryptone broth at 26°C. Nucleotide sequence of this strain has been deposited in the GenBank under accession number GU930359. This endophytic bacterium produced 2.37 μMol/mL of indole acetic acid and siderophore as it metabolites. This strain could solubilize phosphate molecules and fixes atmospheric nitrogen. EndophyticS. aquimarinaSjAM16103 was inoculated into four different plants underin vitromethod to analyse its growth-promoting activity and role inside the host plants. The growth of endophyticS. aquimarinaSjAM16103 inoculated explants were highly significant than the uninoculated control explants. Root hairs and early root development were observed in the endophyticS. aquimarinaSjAM16103 inoculated explants.

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“…Findings by Zuraida et al [17] showed that sub-culture of microshoots in a medium with 1 mg/L BAP produced the highest proliferation of pineapple plantlets. Furthermore, Alderete et al [18] reported that the nodal segments of Mecardonia tenella cultured in a medium with 1 mg/L BAP showed the best multiplication rates, with values around 29 shoots per explant. The positive effect of BAP and NAA in promoting the proliferation of shoots was also detected by Boulay [19], who indicated that the best shoot proliferation of Sequoia sempervirens occurred on MS medium with 0.5 -1.0 mg/L BAP and 0.02 mg/L NAA.…”

Section: Figure 1 Micropropagation Of Vanilla Planifolia On Ms Mediumentioning

confidence: 99%

A Simple and Efficient Protocol for the Mass Propagation of <i>Vanilla planifolia</i>

Zuraida¹,

Izzati²,

Nazreena³

et al. 2013

AJPS

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The present study describes a direct shoot regeneration-based micropropagation procedure for Vanilla planifolia. Two types of explant (i.e. shoot apex and stem nodal segment) were screened for their shoot induction potential following a three-month treatment with 6-benzylaminopurine (BAP) and -Naphthaleneacetic acid (NAA). Results indicated that the shoot apices were poor candidates for shoot induction whereas the stem nodal segments showed potential for shoot initiation at a rate of up to 6 shoots/explant. Stem nodal segments were the most responsive as shoots formed (55 shoots) directly following treatments with 1 mg/L BAP at half strength MS medium after the third subculture. In addition, more shoots were produced on solid medium treatments compared to the liquid medium treatments in two strengths of mediums tested. Regenerated plantlets derived from the 1 mg/L of BAP treatment were induced to root following a one month culture in growth regulator-free MS medium. There was 90% survival rate of the rooted plantlets after acclimatization in the greenhouse. The findings in the present study would be helpful for large-scale mass propagation of Vanilla planifolia using this simple and efficient protocol.

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Establishment of an in vitro micropropagation protocol for Mecardonia tenella

Cited by 7 publications

References 3 publications

An elite protocol for accelerated quality-cloning in Gerbera jamesonii Bolus cv. Sciella

An elite protocol for accelerated quality-cloning in Gerbera jamesonii Bolus cv. Sciella

Plant Growth Promoting of EndophyticSporosarcina aquimarinaSjAM16103 Isolated from the Pneumatophores ofAvicennia marina L.

A Simple and Efficient Protocol for the Mass Propagation of <i>Vanilla planifolia</i>

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Establishment of an in vitro micropropagation protocol for Mecardonia tenella

Cited by 7 publications

References 3 publications

An elite protocol for accelerated quality-cloning in Gerbera jamesonii Bolus cv. Sciella

An elite protocol for accelerated quality-cloning in Gerbera jamesonii Bolus cv. Sciella

Plant Growth Promoting of EndophyticSporosarcina aquimarinaSjAM16103 Isolated from the Pneumatophores ofAvicennia marina L.

A Simple and Efficient Protocol for the Mass Propagation of &lt;i&gt;Vanilla planifolia&lt;/i&gt;

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A Simple and Efficient Protocol for the Mass Propagation of <i>Vanilla planifolia</i>