A Simple and Efficient Protocol for the Mass Propagation of <i>Vanilla planifolia</i>

Abstract

The present study describes a direct shoot regeneration-based micropropagation procedure for Vanilla planifolia. Two types of explant (i.e. shoot apex and stem nodal segment) were screened for their shoot induction potential following a three-month treatment with 6-benzylaminopurine (BAP) and -Naphthaleneacetic acid (NAA). Results indicated that the shoot apices were poor candidates for shoot induction whereas the stem nodal segments showed potential for shoot initiation at a rate of up to 6 shoots/explant. Stem nodal segments were the most responsive as shoots formed (55 shoots) directly following treatments with 1 mg/L BAP at half strength MS medium after the third subculture. In addition, more shoots were produced on solid medium treatments compared to the liquid medium treatments in two strengths of mediums tested. Regenerated plantlets derived from the 1 mg/L of BAP treatment were induced to root following a one month culture in growth regulator-free MS medium. There was 90% survival rate of the rooted plantlets after acclimatization in the greenhouse. The findings in the present study would be helpful for large-scale mass propagation of Vanilla planifolia using this simple and efficient protocol.

Effect of LED light quality on in vitro shoot proliferation and growth of vanilla (Vanilla planifolia Andrews)

“…Therefore, there is a need to search for an alternative way to mass produce this plant species. Recently, several studies have reported on the micropropagation of V. planifolia (Tan et al, 2011;Mengesha et al, 2012;Zuraida et al, 2013;De Oliveira et al, 2013). Prior to the present study, the effect of FL on in vitro propagation of vanilla has been reported but no research had been reported using LEDs.…”

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

“…In addition to their unquestionable botanical and ecological importance, orchids participate in current cultivation systems using high-tech horticulture, grown in environments with good climate control, especially temperature, which allows the induction of flowering regardless of the time of year, especially aiming at the scheduled supply of potted and cut flowers in the competitive world flower market. Some species of orchids, such as the genera Dendrobium, Gastrodia, and Bletilla, have also been used for medicinal purposes, using the basis of traditional Chinese medicine [4] and some Vanilla species is also used for food purposes [5].…”

Desinfección de segmentos nodales sobre el rendimiento morfogenético de vitroplantas de Vanilla planifolia Andrews

“…Los resultados de este trabajo en cuanto a la respuesta morfogenética de la longitud del brote, en ausencia de reguladores de crecimiento, fueron muy superiores a los indicados en algunos procedimientos de otros autores (Giridhar y Ravishankar, 2004;Tan et al, 2011b;Zuraida et al, 2013). El promedio logrado (10,54 ± 0,89 cm) no se ha obtenido en protocolos con periodos de evaluación de sesenta días, aún con el uso de reguladores de crecimiento.…”

“…Al respecto, se han obtenido valores de 4,20 ± 0,14 cm al utilizar un medio MS complementado con 1 mg/l BAP (Tan et al, 2011b), 3,8 ± 0,2 cm en un medio MS complementado con 1 mg/l BAP + 0,5 mg/l ANA (Tan et al, 2011a) y 2,9 ± 0,19 cm en un medio MS complementado con 5 mg/l BAP + 1 mg/l AFA (ácido fenilacético) (Giridhar et al, 2003). Otros protocolos similares con un periodo de evaluación de 45 días originaron valores de 4,5 ± 0,14 cm (Zuraida et al, 2013), 2,6 ± 0,1 cm (Giridhar y Ravishankar, 2004) y 3,81 ± 0,33 cm (Giridhar et al, 2001).…”

“…La validez de la asepsia durante la desinfección para el cultivo in vitro de plantas tropicales ha sido documentada de forma limitada, a pesar de que esta estrategia es un factor clave para lograr el establecimiento y multiplicación óptima de estas especies (Mng'omba et al, 2012). En el caso de la vainilla, existen diversidad de protocolos de micropropagación donde se mencionan procedimientos de desinfección (Tan et al, 2011a;Zuraida et al, 2013;Mujar et al, 2014). No obstante, la literatura no indica la efectividad de tales procesos, con excepción del trabajo de Abebe et al (2009), quienes lograron un 90% de explantes libres de contaminación al utilizar 0,1% HgCl 2 , de los cuales, solo el 60% sobrevivió a la desinfección.…”