Effect of LED light quality on in vitro shoot proliferation and growth of vanilla (Vanilla planifolia Andrews)

Bello-Bello, Jericó Jabín; MartÃ­nez-Estrada, Eduardo; Caamal-VelÃ¡zquez, JosÃ© Humberto; Morales-Ramos, Victorino

doi:10.5897/ajb2015.14662

Cited by 49 publications

(20 citation statements)

References 23 publications

(26 reference statements)

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“…In particular, the presence of the green light portion of a spectrum impairs the blue light response (Folta and Maruhnich 2007). As for V. planifolia, white LED light provided the highest chlorophyll content (Bello-Bello et al 2016), which could also be considered for future research with the species used in the present study. Nevertheless, it can be assumed that light quality may affect the production of metabolites in the species tested, although more experiments in this field are necessary.…”

Section: Resultsmentioning

confidence: 96%

“…For example, in Chrysanthemum and Gerbera, red light decreased microshoot dry matter (Kurilčik et al 2008;Gӧk et al 2016). On the other hand, in V. planifolia, the application of blue or red LED light alone resulted in the lowest dry matter, while FL, white LED, and blue:red mixture (1:1) increased this parameter (Bello-Bello et al 2016). The lack of significant differences in dry matter content in other plant species tested here could have resulted from non-sufficient gas exchange in the culture jars (Chen and Chen 2002), or from the presence of multichromatic wavelengths.…”

Section: Resultsmentioning

confidence: 97%

“…Therefore, it is difficult to indicate a single light spectrum suitable for the propagation of every plant species. For example, Bello-Bello et al (2016) reported that blue and red LED light alone decreased the proliferation of Vanilla planifolia Andrews, while white LED, the mixture of blue and red LED, and FL provided the highest propagation ratio. As for Panax vietnamensis Ha et Grushv., the propagation ratio under red:blue LED light in 60:40 ratio was reported to be two times higher than under FL (11.21 and 5.8, respectively; Nhut et al 2015).…”

Section: Resultsmentioning

confidence: 99%

“…There are many studies focused on LED light spectrum optimization with several ornamental and usable plant species (Azmi et al 2016;Bantis et al 2016;Bello-Bello et al 2016). Most of the research focused on various narrow-spectrum red:blue light ratios, which coincide to two areas of the chlorophyll absorption curve, and thus, are essential for photosynthesis (Gupta and Jatothu 2013;Manivannan et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Application of wide-spectrum light-emitting diodes in micropropagation of popular ornamental plant species: a study on plant quality and cost reduction

Miler

Kulus

Wozny

et al. 2018

In Vitro Cell.Dev.Biol.-Plant

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In the present study, the applicability of four wide-spectrum light-emitting diodes (LEDs) emitting warm light (AP67, AP673L, G2, and NS1) was determined for the micropropagation of five popular ornamental plant species: Chrysanthemum × grandiflorum, Gerbera jamesonii, Heuchera × hybrida, Ficus benjamina, and Lamprocapnos spectabilis. Plantlets were grown in a growth room with a 16-h photoperiod. The photosynthetic photon flux density was set at 62-65 μmol m −2 s −1. The composition of the media and subculture timing were adjusted to the needs of each species tested. The results were compared to the cool daylight-emitting fluorescent (FL) control (TLD 36W/54). In most of the species studied (except for F. benjamina), the highest propagation ratios, or ratios similar to the FL control, were observed under the red-and far-red-abundant G2 LEDs. NS1 spectrum (with the highest proportion of blue and green light) was also efficient for G. jamesonii and L. spectabilis, and it provided a similar propagation ratio as the FL control. Light quality affected shoot length, number of leaves, callus regeneration, and the biosynthesis of chlorophyll. This influence, however, was species-dependent. Lighting conditions did not affect the dry matter and rooting in most of the species tested, except for G. jamesonii. The substitution of FLs with G2 LEDs can result in a 50% reduction of annual electricity costs, while the application of NS1 lamps can generate savings of up to 75%. In conclusion, the G2 LED lighting system seemed to be the most suitable in terms of propagation efficiency, plantlet quality, and cost reduction.

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Application of wide-spectrum light-emitting diodes in micropropagation of popular ornamental plant species: a study on plant quality and cost reduction

Miler

Kulus

Wozny

et al. 2018

In Vitro Cell.Dev.Biol.-Plant

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“…The photosynthetic ability of in vitro plantlets [7] can be improved by changing the light fluence rate and quality in the growth environment [8]. Light-emitting diodes (LED) have been used to accelerate plantlet growth and their effects on chlorophyll synthesis [9, 10], photosynthesis [11, 12], and morphogenesis [5, 9, 13–15] have been studied in a variety of species.…”

Section: Introductionmentioning

confidence: 99%

Application of 3D printing to prototype and develop novel plant tissue culture systems

et al. 2017

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BackgroundDue to the complex process of designing and manufacturing new plant tissue culture vessels through conventional means there have been limited efforts to innovate improved designs. Further, development and availability of low cost, energy efficient LEDs of various spectra has made it a promising light source for plant growth in controlled environments. However, direct replacement of conventional lighting sources with LEDs does not address problems with uniformity, spectral control, or the challenges in conducting statistically valid experiments to assess the effects of light. Prototyping using 3D printing and LED based light sources could help overcome these limitations and lead to improved culture systems.ResultsA modular culture vessel design in which the fluence rate and spectrum of light are independently controlled was designed, prototyped using 3D printing, and evaluated for plant growth. This design is compatible with semi-solid and liquid based culture systems. Observations on morphology, chlorophyll content, and chlorophyll fluorescence based stress parameters from in vitro plants cultured under different light spectra with similar overall fluence rate indicated different responses in Nicotiana tabacum and Artemisia annua plantlets. This experiment validates the utility of 3D printing to design and test functional vessels and demonstrated that optimal light spectra for in vitro plant growth is species-specific.Conclusions3D printing was successfully used to prototype novel culture vessels with independently controlled variable fluence rate/spectra LED lighting. This system addresses several limitations associated with current lighting systems, providing more uniform lighting and allowing proper replication/randomization for experimental plant biology while increasing energy efficiency. A complete procedure including the design and prototyping of a culture vessel using 3D printing, commercial scale injection molding of the prototype, and conducting a properly replicated experiment are discussed. This open source design has the scope for further improvement and adaptation and demonstrates the power of 3D printing to improve the design of culture systems.Electronic supplementary materialThe online version of this article (doi:10.1186/s13007-017-0156-8) contains supplementary material, which is available to authorized users.

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Advances in Somatic Embryogenesis in Vanilla (Vanilla planifolia Jacks.)

Ramírez-Mosqueda

Bello-Bello

Armas-Silva

et al. 2022

Methods in Molecular Biology

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Effect of LED light quality on in vitro shoot proliferation and growth of vanilla (Vanilla planifolia Andrews)

Cited by 49 publications

References 23 publications

Application of wide-spectrum light-emitting diodes in micropropagation of popular ornamental plant species: a study on plant quality and cost reduction

Application of wide-spectrum light-emitting diodes in micropropagation of popular ornamental plant species: a study on plant quality and cost reduction

Application of 3D printing to prototype and develop novel plant tissue culture systems

Advances in Somatic Embryogenesis in Vanilla (Vanilla planifolia Jacks.)

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