Advances in Somatic Embryogenesis in Vanilla (Vanilla planifolia Jacks.)

Ramírez-Mosqueda, Marco A.; Bello-Bello, Jericó Jabín; Armas-Silva, Arturo A; Rodríguez-Deméneghi, Marco Vinicio; Martínez-Santos, Eduardo

doi:10.1007/978-1-0716-2485-2_3

Cited by 3 publications

(1 citation statement)

References 77 publications

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“…Entre estos, Mathew et al (2000) señala un protocolo comercialmente viable para la propagación masal in vitro de V. tahitensis, utilizando bencilaminopurina (1 mg/L) y ácido naftalenacético (ANA) 0.1 mg/L, durante 60 a 70 d de cultivo. Dado que V. tahitensis es un híbrido entre V. planifolia y V. odorata (Soto-Arenas y Dressler, 2010), se hace referencia a antecedentes de micropropagación de V. planifolia, en la cual se han reportado numerosos estudios de multiplicación (Ramírez-Mosqueda et al, 2022), a partir del cultivo de nudos de tallos (Chin et al, 2011;Giridhar et al, 2001), ápices de raíces (Philip y Nainar, 1986), ápices de vástagos (Giridhar et al, 2001, Kalimuthu et al, 2006 y cultivo de callos (Janarthanam and Seshadri, 2008;Chin et al, 2011). Recientemente Manokari et al (2022) indicaron que el uso de nitrato de plata promovió la embriogénesis somática y la regeneración de plántulas en explantes de hojas.…”

Section: Introductionunclassified

Effect of different concentrations of benzylaminopurine for the in vitro propagation of Vanilla tahitensis (J. W. Moore)

Parismoreno-Rivas,

Barcia-Jalca,

Medina-Litardo

et al. 2024

Mexjbiotechnol

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The establishment of an efficient in vitro propagation protocol is still ongoing for Vanilla tahitensis (J. W. Moore), an underdeveloped but economically attractive crop in Ecuador. The objective of the research was to evaluate the application of different concentrations of cytokine benzylaminopurine (BAP) in its in vitro culture. The effect of six concentrations of BAP was studied (0.50, 1.00, 1.50, 1.75, 2.00 and 2.50 mg/L), and a control treatment, with five repetitions, using a completely randomized design. The 2.0 mg/L concentration of BAP allowed the best micropropagation of the vanilla producing 5 and 6 shoots at 60 and 90 d after sowing. The greatest length of roots was achieved with 1.75 mg/L (7 cm). Regarding plant height and leaf number, the 2.0 mg/L concentration showed the highest plant height with 18 and 6 cm at 90 and 60 d after sowing, and the highest number of leaves with 7 and 5 leaves, respectively. This research reports a successful in vitro culture protocol for this species, to promote the development of this crop in Ecuador, due to its outstanding possibilities for commerce at the international market.

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