An elite protocol for accelerated quality-cloning in Gerbera jamesonii Bolus cv. Sciella

Abstract

A novel, efficient, and simple protocol was developed on in vitro mass propagation and acclimatization of Gerbera jamesonii Bolus cv. Sciella, an ornamental plant with attractive flowers. Shoot tip was used as the primary explant for in vitro establishment in which Murashige and Skoog (MS) medium supplemented with a low level of NAA (0.5 mgl −1 ) and BAP (1.5 mgl −1 ) promoted earliest axillary bud initiation within 5 d in 91.6% of the inoculants. Five axillary buds were initiated from a single explant within 13 d after inoculation. A very high rate of shoot multiplication (14 shoots per inoculated axillary bud) and proliferation was achieved when MS medium was fortified with a relatively higher level of BAP (2 mgl −1 ) and 60 mgl −1 ADS within 27 d of multiple shoot culture. A maximum number of well-developed roots per plant was observed in MS medium with 0.5 mg l −1 IAA in the next 26 d. In the easy low-cost acclimatization process of 20 d, a combination of sand, soil, cow urine, and tea leaves extract (1:1:1:1; v/v) ensured 95% survival rate. Sixty-one well-acclimatized plants were obtained from a single shoot tip within 86 d. The sustained multiple shoot culture for 15 mo paved the way toward the conservation of genetic resources as well as beneficial economics. The clonal fidelity study of micropropagated and sustained cultured clones using ISSR primers ensured the continuous supply of quality propagules retaining genetic uniformity. The in vitro-generated plants performed better over conventionally propagated plants in the field condition.

Influence of Different Organic Waste Materials on Hardening of Micropropagated Tea (Camellia sinensis L.) Clone 'Iran 100'

“…4) as well as good plant development based on other morphological parameters considered. The efficiency of tea extract on acclimatization of micropropagated plants was also reported earlier 21 where the positive influence of tea extract on retention of optimum humidity and induction of new rhizosphere under ex vitro condition was observed. The correlation of electrical conductivity, growth factors and pH were computed to determine the relationship among them.…”

A two step method for accelerated mass propagation of Dendrocalamus asper and their evaluation in field

“…It is clear from our study, in vitro-generated D. asper performed very promisingly. The probable reason of quality performance of in vitro-generated plants would be the complete expression of morphogenetic potential from the diseasefree, favorable environment in which they were exposed during in vitro propagation (Gantait et al 2010b) and during their cultivation in vivo.…”

In vitro biotechnological advancements in Malabar nut (Adhatoda vasica Nees): Achievements, status and prospects

“…Inclusion of common compost, vermicompost or farmyard manure enhanced the rate of acclimatization and increased the survival rate. According to Gantait et al [57], compost and farmyard manure plays a major role in retention of moisture of the substrate apart from nutrient supply. It has already been established in several reports that retention of high humidity is a key component for high frequency acclimatization.…”