Abstract:Avaliou-se a esporulação de conídios de Mycosphaerella fijiensis (isolado LPM472) em sete meios de cultura (batata dextrose ágar, V8 ágar, V8 CaCO3 ágar, água de coco ágar, batata cenoura ágar, folha de banana ágar e micophil) sob quatro regimes de luminosidade (escuro contínuo, fotoperíodo de 12 h, luz contínua e seqüencial - dez dias no escuro e cinco dias sob luz contínua). O ensaio foi conduzido em delineamento inteiramente casualizado, com cinco repetições. A esporulação foi determinada em erlenmeyer de 1… Show more
“…A ausência de luz para indução da esporulação já foi relatada para diversos fungos fitopatogênicos como, Alternaria solani (LUKENS, 1963) e Mycosphaerella fijensis (HANADA et al, 2002). Nestes casos, a luz age como foto-inibidor do crescimento micelial.…”
Section: Resultsunclassified
“…Para serem desenvolvidos estudos de resistência genética do hospedeiro, há necessidade de se fazer inoculações artificiais em ambientes controlados, o que requer a produção massal de esporos do patógeno (HANADA et al, 2002). Porém, poucas são as informações referentes à esporulação de F. solani da mandioca in vitro.…”
Resumo -Visando a caracterização fisiológica de Fusarium solani isolado de raízes de mandioca, objetivou-se com este trabalho avaliar a esporulação e o crescimento micelial de F. solani em diferentes meios de cultura e regimes de luminosidade. O fungo foi cultivado utilizando cinco meios de cultura (batata dextrose ágar, batata sacarose ágar, mandioca ágar, micophil e ágar-água) sob três regimes de luminosidade (escuro contínuo, fotoperíodo de 12 h e luz contínua) durante o período de incubação de sete dias, a temperatura de 25 °C ± 2 o C. O ensaio foi conduzido em delineamento inteiramente casualizado, em esquema fatorial (5x3), com três repetições. Discos de 5 mm de diâmetro, retirados da borda da colônia cultivada em meio BDA, foram transferidos para o centro de placas de Petri contendo 20 mL de cada meio. Determinou-se o crescimento micelial por meio da medida do diâmetro das colônias em dois sentidos diametralmente opostos, enquanto a esporulação por meio da quantificação de conídios pelo método da gota. Foram observadas variações significativas na produção de massa micelial e conídios nos diferentes meios de cultura e regimes de luminosidade testados, sendo que BDA e BSA sob regime de luz contínua induziram maior crescimento micelial e produção de conídios. Enquanto que no meio AA sob escuro contínuo ocorreu às menores taxas de esporulação e crescimento micelial.
Palavras-chaves -Avaliação. Fisiologia. Manihot esculenta. Podridão radicular.Abstract -Considering the physiological characterization of Fusarium solani isolated from cassava roots, the objective of this study was to evaluate mycelial growth and sporulation of F. solani in different culture media and lighting regimes. The fungus was grown using five culture media (potato dextrose agar, potato sucrose agar, cassava, agar-agar, and water micophil) under three light regimes (continuous darkness, a photoperiod of 12 h, and continuous light) during the incubation period of seven day, temperature 25 °C ± 2 °C. The trial was done in completely randomized design with three replications. Discs of 5mm diameter taken from the edge of the colony grown on PDA medium were transferred to the center of Petri dishes containing 20 mL of each medium. Mycelial growth was determined by measuring the diameter of the colonies in two diametrically opposite directions while sporulation by quantifying conidia by drop method. No significant changes in the production of conidia and mycelial mass in different culture media and lighting regimes tested, and BDA and BSA under the regime of continuous light best sporulation and conidial production were observed. While in the midst AA under continuous darkness was the lowest rates of mycelial growth and sporulation.
“…A ausência de luz para indução da esporulação já foi relatada para diversos fungos fitopatogênicos como, Alternaria solani (LUKENS, 1963) e Mycosphaerella fijensis (HANADA et al, 2002). Nestes casos, a luz age como foto-inibidor do crescimento micelial.…”
Section: Resultsunclassified
“…Para serem desenvolvidos estudos de resistência genética do hospedeiro, há necessidade de se fazer inoculações artificiais em ambientes controlados, o que requer a produção massal de esporos do patógeno (HANADA et al, 2002). Porém, poucas são as informações referentes à esporulação de F. solani da mandioca in vitro.…”
Resumo -Visando a caracterização fisiológica de Fusarium solani isolado de raízes de mandioca, objetivou-se com este trabalho avaliar a esporulação e o crescimento micelial de F. solani em diferentes meios de cultura e regimes de luminosidade. O fungo foi cultivado utilizando cinco meios de cultura (batata dextrose ágar, batata sacarose ágar, mandioca ágar, micophil e ágar-água) sob três regimes de luminosidade (escuro contínuo, fotoperíodo de 12 h e luz contínua) durante o período de incubação de sete dias, a temperatura de 25 °C ± 2 o C. O ensaio foi conduzido em delineamento inteiramente casualizado, em esquema fatorial (5x3), com três repetições. Discos de 5 mm de diâmetro, retirados da borda da colônia cultivada em meio BDA, foram transferidos para o centro de placas de Petri contendo 20 mL de cada meio. Determinou-se o crescimento micelial por meio da medida do diâmetro das colônias em dois sentidos diametralmente opostos, enquanto a esporulação por meio da quantificação de conídios pelo método da gota. Foram observadas variações significativas na produção de massa micelial e conídios nos diferentes meios de cultura e regimes de luminosidade testados, sendo que BDA e BSA sob regime de luz contínua induziram maior crescimento micelial e produção de conídios. Enquanto que no meio AA sob escuro contínuo ocorreu às menores taxas de esporulação e crescimento micelial.
Palavras-chaves -Avaliação. Fisiologia. Manihot esculenta. Podridão radicular.Abstract -Considering the physiological characterization of Fusarium solani isolated from cassava roots, the objective of this study was to evaluate mycelial growth and sporulation of F. solani in different culture media and lighting regimes. The fungus was grown using five culture media (potato dextrose agar, potato sucrose agar, cassava, agar-agar, and water micophil) under three light regimes (continuous darkness, a photoperiod of 12 h, and continuous light) during the incubation period of seven day, temperature 25 °C ± 2 °C. The trial was done in completely randomized design with three replications. Discs of 5mm diameter taken from the edge of the colony grown on PDA medium were transferred to the center of Petri dishes containing 20 mL of each medium. Mycelial growth was determined by measuring the diameter of the colonies in two diametrically opposite directions while sporulation by quantifying conidia by drop method. No significant changes in the production of conidia and mycelial mass in different culture media and lighting regimes tested, and BDA and BSA under the regime of continuous light best sporulation and conidial production were observed. While in the midst AA under continuous darkness was the lowest rates of mycelial growth and sporulation.
“…Thus, sporulation is induced in filamentous fungi. A long period of light can also exert an influence on the activation of key enzymes [28] responsible for the synthesis of compounds in the culture media that are essential to the sporulation of different fungal species [29].…”
Section: Discussionmentioning
confidence: 99%
“…This medium is composed of eight vegetables (tomato, beet, celery, carrot, lettuce, spinach, parsley and watercress) and is rich in potassium, complex carbohydrates, minerals, fibre and vitamins A, E and C, with only 0.3% sugar, which are essential constituents for the obtainment of high sporulation in different fungal species [29]. According to Brunelli et al [30] for sporulation to occur in different species of Alternaria, the medium needs to have low sugar content [32].…”
The tangerine pathotype of Alternaria alternata is the aetiological agent of Alternaria brown spot on tangerines. In the state of Paraíba, Brazil, its occurrence on "Dancy" tangerine trees is associated with genetic aspects as well as the influence of environmental conditions on reproduction and dissemination within and between populations. The aim of the present study was to evaluate the diversity of isolates of this pathogen using morphophysiological and molecular markers. For the analysis of mycelial growth and sporulation, 30 isolates from different locations were examined at 24-hour intervals until the seventh day, when the spores were quantified. The 30 isolates were characterised based on molecular markers (ISSR) and genetic similarity (Jaccard index). A factor arrangement was used: 30 isolates, four media (ODA, PDA, LEA and V8), three light regimes (continuous dark, alternating light and continuous light) and three temperatures (15˚C, 25˚C and 35˚C), with 12 repetitions. Groups 1, 2 and 3 presented low genetic variability. Group 4 showed high genetic variability of the isolates obtained from the Massaranduba (state of Paraíba-Brazil) producing region and higher mycelial growth and sporulation of A. alternata. The continuous light regime and the temperature 25˚C in PDA and V8 media were the ideal conditions for the mycelial growth and sporulation, respectively, of the isolates of A. alternata.
“…Studies on the effect of culture media, light regimes and temperatures for culturing fungi have already been conducted on some fungi, such as Mycosphaerella fijiensis (Morelet) Deighton (Hanada et al, 2002), Cercospora zeae-maydis Tehon & Daniels (Brunelli et al, 2006), Magnaporthe grisea (Dias Neto et al, 2010), Cercospora coffeicola Berk. & Cooke (Silva et al, 2016), Pseudocercospora vitis (Lév.)…”
Sirosporium diffusum is the causal agent of the brown leaf spot disease on pecan trees that seriously damages the foliage of adult plants and seedlings. This fungal species is difficult to grow satisfactorily in a culture medium. Therefore, the aim of this study was to evaluate the effects of different physical conditions on the development of S. diffusum. In the first assay, eight culture media and five light regimes were combined, while in the second, the three treatments that promoted highest sporulation were combined with three temperatures. The trials were conducted in a two-factorial arrangement in a fully randomized design with six replicates. V8, V8CaCO 3, and CA media under a 24-h photoperiod produced the highest respective sporulations: 29 × 10 4 , 35 × 10 4 , and 41 × 10 4 conidia ml -1. The best temperature for sporulation was 20±1 °C for all culture media, especially V8CaCO 3 and CA. The best artificial conditions for obtaining good mycelial growth and sporulation consisted of a photoperiod of 24 h, temperature of 20±1 °C and V8CaCO 3 or CA culture medium.
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