Erythropoietin ameliorates genetamicin-induced renal toxicity: A biochemical and histopathological study

Abstract

Background:Investigations have attempted to modify the outcome of tubular injury by either ameliorating renal tubular damage or promoting tubular regeneration in the case of acute tubular necrosis. Objectives: We investigated the protective effect of Eprex an erythropoietin analogue on tubular injury induced by gentamicin (GM). Materials and Methods: Forty male Wistar rats were randomly divided into four groups. In group 1,rats were served as a sham group. In group 2, rats were injected intraperitoneally with 100 mg/kg of GM for 10 consecutive days (positive control group) and then were sacrificed. In group 3, rats received GM for 10 days then Eprex 100U/kg was injected intraperitoneally for the next 10 days and then they were sacrificed at the day 20th. In group 4 rats were injected a combination of GM (80 mg/kg) and Eprex 100U/kg intraperitoneally for 10 days and then were sacrificed. Results: The results indicated that, Eprex prevented the increase in serum creatinine (Cr) and blood urea nitrogen (BUN). The effect of Eprex on damage score, showed that co-administration of GM and Eprex (group 3 and 4) reduced the kidney tissue damage compared to positive control group (P<0.05). This result indicat that Eprex potentially can reduce or prevent the kidney tissue damage. Conclusions: Ameliorative effect of Eprex when the drug was given in combination with GM and also when the drug was applied after GM-induced tubular damage, revealed the renoprotective potency of Eprex. Eprex is a promising drug to prevent or attenuate tubular damage induced by GM or other nephrotoxic agents which act through the same mechanisms as gentamicin.

C-Phycocyanin attenuates cisplatin-induced nephrotoxicity in mice

“…4 We concluded that gender difference of cisplatin renal toxicity was related to the reninangiotensin system receptors in the kidneys. [4][5][6][7][8][9] Moreover, we recently found that vitamin C, vitamin E, or losartan have no ameliorative effects against cisplatin-kidney toxicity in presence of estrogen in ovariectomized rat model of cisplatin toxicity, 10 which is in agreement with our previous findings too. Hence, it is well established that there is a gender difference in the cisplatin renal toxicity in rat model.…”

“…Hence, it is well established that there is a gender difference in the cisplatin renal toxicity in rat model. Indeed, it is well recognized that some conditions that lead to chronic kidney diseases are sex related too; [5][6][7][8][9][11][12][13] however, few studies published regarding gender difference in cisplatin renal toxicity . However, there still needs to be more investigations, mechanisms take part in cisplatin renal toxicity especially on gender difference.…”

Inhibition of gentamicin–induced renal tubular cell necrosis

“…Usage of antioxidants improved serum creatinine, blood urea nitrogen, creatinine clearance and histological injuries such as tubular necrosis, tubular cell edema and apoptosis in gentamicin injected rats (6,7). In the recent study, Rafieian-Kopaei and colleagues investigated the effect of erythropoietin in inhibition of gentamicin nephrotoxicity (8). Although erythropoietin acts as hemopoietic factor but this hormone show abilities such as anti inflammation, anti apoptosis (9), malondialdehyde lowering and increasing of catalase activity (10).One of the advantages of Rafieian-kopaei et al study is treatment of rats for 10 days by erythropoietin after inducing gentamicin nephrotoxicity and besides co-treatment of gentamicin and erythropoietin at 10 days simultaneously.Rafieian-Kopaei and colleagues showed that erythropoietin improved significantly serum creatinine and blood urea nitrogen in gentamicin injected rats simultaneously and even after gentamicin nephrotoxicity induction.…”

Erythropoietin; bright future and new hopes for an old drug

“…In recent years there have been many studies on the mechanisms of GM-induced nephrotoxicity and reduction of the renal damage caused by it (3,4). issue of journal, the effects of erythropoietin on amelioration of gentamicin-induced renal toxicity was investigated by biochemical and histopathological indices (5). A renoprotective effect for Eprex an analogue of erythropoietin was shown when the drug was given in combination with gentamicin.…”