Enzymological Evaluation of Hepatotropic Effect of Ozone in a Subchronic Experiment

Peretyagin, S. P.; Мартусевич, А. К.; Соловьева, А. Г.; Zimin, Yu. V.; Peretyagin, P. V.

doi:10.1007/s10517-013-2057-9

Cited by 2 publications

(4 citation statements)

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“…At the same time, the severity of the microcirculation response was directly determined by the amount of ozone introduced, and we fixed non-linear dependence on it. This confirms the non-linear nature of the metabolic shifts that we obtained for these concentrations in previous studies 5,6 . Evaluating the integrative indicator of microcirculation state (microcirculation index), it should be noted that the most pronounced stimulating effect was established for the minimum concentration of ozone (3000 mcg/l).…”

Section: Discussionsupporting

confidence: 92%

“…throughout 30 days. Animals of other groups (groups 2 -4, n = 10 per group) received 30 intraperitoneal infusions (1 ml/day) with ozonized saline solution (saturating ozone concentration for indicated groups -3000, 10,000 and 40,000 mcg/l, ozone dose per procedure -0.75, 2.5 and 10 mcg, respectively 5,6 ). Ozone and oxygen mixture was produced with "Medozons-System" ozonator (Nizhny Novgorod, Russia).…”

Section: Animals and Exposuresmentioning

confidence: 99%

“…The sanogenic potential of ozone in gas phase and different ozonized solutions has been demonstrated in many clinical and experimental investigations [1][2][3] . It is proposed that the trigger mechanism of ozone biological action includes the influence of oxidative metabolism of blood and tissues with moderate stimulation of lipid peroxidation and predominant activation of the antioxidant system [4][5][6][7] . These effects initiate the complex of metabolic reaction , such as optimization of cell energy production, intensification of oxidative and enzyme detoxication, etc.…”

Section: Introductionmentioning

confidence: 99%

“…These effects initiate the complex of metabolic reaction , such as optimization of cell energy production, intensification of oxidative and enzyme detoxication, etc. 1,[5][6][7][8][9][10][11][12] .…”

Section: Introductionmentioning

confidence: 99%

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Microcirculatory effects of systemic metabolic correction with reactive oxygen species: An experimental study

Мартусевич

Karuzin²,

Dilenyan

et al. 2020

Biomed. Res. Ther.

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Introduction: The purpose of this study is to estimate microcirculation changes under systemic (intraperitoneal) administration of ozonized saline. Methods: For this study, we formed four equal groups of Wistar male rats (n = 10 per group). Rats of first (control) group was intraperitoneally injected with non-ozonized sterile saline solution (daily administration volume — 1 ml.) during 30 days. Animals of other groups (n = 10 for second, third and fourth groups) received 30 intraperitoneal infusions (1 ml/day) daily with ozonized saline solution (saturating ozone concentration for indicated groups — 3000, 10000 and 40000 mcg/l, ozone dose per procedure — 0.75, 2.5 and 10 mcg, respectively). Technology of the Laser Doppler Flowmetry (LDF) along with ``LAKK-02'' device (Moscow, Russia) was used for complex estimation of skin microcirculation state. This technology allows us to study blood flow intensity in skin microvessels at the first and thirty-first days of the experiment. We also can estimate regulatory mechanisms of microcirculation support and the presence of shunt paths of the microcirculation. Results: We studied the dose-dependent response of microcirculation on ozone infusions in chronic experiments. We found that long-lasting course (30 procedures) of intraperitoneal administration of ozonized sodium chloride solution provides an increase in the level of microcirculation index compared to that of the control (injections of nonozonized saline solution), regardless of the applied dose of ozone. At the same time, the level of the microcirculation response was directly determined by the introduced amount of ozone, and we fixed non-linear dependence on it. Our study allowed us to show that only low doses of ozone (0.75 mcg/day) have proadaptive effects on the microcirculatory bed. This was shown both in the dynamics of the microcirculation index and the state of regulatory mechanisms. Middle ozone dose (2.5 mcg/day) also caused the stimulation of blood flow in small vessels, though this was predominantly through other mechanisms (neurogenic components). The most negative reaction of microcirculation was observed for high ozone dose (8 mcg). Conclusion: Despite the activation of microcirculation observed in this case, we have identified that the mechanism was primarily through the respiratory component regulation and via formation of "steal syndrome" in the tissue.

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Section: Discussionsupporting

confidence: 92%

Section: Animals and Exposuresmentioning

confidence: 99%