Using an integral qualitative and quantitative approach to the studies of initiation of the biological material crystallogenesis, we showed in experiments with normal human saliva that the external characteristics of biological fluid (pH, osmolality, and environmental temperature) determine the results of crystallization (tesigraphic facies). The main external (macroenvironment) and inner (microenvironment) factors of biological fluid crystal formation, determining specific features of the tesigraphic facies, were distinguished and classified. The informative value of differential analysis of biomaterial properties by means of modulating the environmental conditions is established.

Objectives. Using of systemic ozone therapy connecting with antioxidants in early period of burn disease for detoxication and metabolic disorders correction is investigated. Materials and Methods. We used active preventive metabolic detoxication in early period of burn disease (5 th-15 th days after shock stage). It includes, besides complex infusion and transfusion therapy and classic pharmacological treatment, employment of antioxidants with succinic acid (reamberin, mexidol), systemic ozone therapy (intravenous injections of ozonated sodium chloride solution with ozone concentration 120-240 µg/mL; saturating ozone concentration in gas flow-5000 µg/L, sparging speed-2 l/min), major autohemotherapy (twice a week; ozone dose-250-300 µg/mL) and monitor cleansing of bowels with rectal ozone insufflations (ozone dose-1000 µg/mL). Estimation of personal oxidant dose and treatment effectiveness is carried out by biochemiluminescence control of pro-and antioxidant system state. Complex patient study also includes endotoxicosis markers (blood level of glucose, creatinine, bilirubin, urea and intermediate molecular weight peptides), proteolytic enzymes activity (tripsin, antitripsin, elastase), cardiovascular and microcirculation function examination (tetrapolar rheography, spirography, laser Doppler flowmetry) at burn disease dynamics. We formed two patients groups: first control group (20 burned patients) received a traditional therapy; second group (20 burned patients) got a described detoxication complex additional to treatment of first group. All patients had large burns (more than 20% body square). Indications for parenteral ozone therapy are signs of hypoxia (hypoxemia, elevated lactate level, depressed lactate dehydrogenase activity) and toxemia (increased blood level of glucose, creatinine, urea, intermediate molecular weight peptides, fibrine degradation products, lypoperoxidation intensification); cardiovascular dysfunction; respiratory function disorders. Results. Method of ozone dose individualization in intravenous ozone ther

It is known that molecular hydrogen is a relatively stable, ubiquitous gas that is a minor component of the atmosphere. At the same time, in recent decades molecular hydrogen has been shown to have diverse biological effects. By the end of 2022, more than 2000 articles have been published in the field of hydrogen medicine, many of which are original studies. Despite the existence of several review articles on the biology of molecular hydrogen, many aspects of the research direction remain unsystematic. Therefore, the purpose of this review was to systematize ideas about the nature, characteristics, and mechanisms of the influence of molecular hydrogen on various types of cells, including stem cells. The historical aspects of the discovery of the biological activity of molecular hydrogen are presented. The ways of administering molecular hydrogen into the body are described. The molecular, cellular, tissue, and systemic effects of hydrogen are also reviewed. Specifically, the effect of hydrogen on various types of cells, including stem cells, is addressed. The existing literature indicates that the molecular and cellular effects of hydrogen qualify it to be a potentially effective agent in regenerative medicine.

Currently, plasma medicine is a synthetic direction that unites the efforts of specialists of various profiles. For the successful formation of plasma medicine, it is necessary to solve a large complex of problems, including creating equipment for generating cold plasma, revealing the biological effects of this effect, as well as identifying and justifying the most promising areas of its application. It is known that these biological effects include antibacterial and antiviral activity, the ability to stimulate hemocoagulation, pro-regenerative properties, etc. The possibility of using the factor in tissue engineering and implantology is also shown. Based on this, the purpose of this review was to form a unified understanding of the biological effects and biomedical applications of argon cold plasma. The review shows that cold plasma, like any other physical and chemical factors, has dose dependence, and the variable parameter in this case is the exposure of its application. One of the significant characteristics determining the specificity of the cold plasma effect is the carrier gas selection. This gas carrier is not just an ionized medium but modulates the response of biosystems to it. Finally, the perception of cold plasma by cellular structures can be carried out by activating a special molecular biosensor, the functioning of which significantly depends on the parameters of the medium (in the field of plasma generation and the cell itself). Further research in this area can open up new prospects for the effective use of cold plasma.

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Applying human biological evolution to solve topical problems of medicine and preventive cardiology was inspired by the realization of the need for clinical and experimental studies of biological (evolutionary) prerequisites in the occurrence of a pathology. Although it has been stated that there is a need to provide a full biological understanding of features, including those that increase an animal’s vulnerability to diseases, unfortunately, in this regard, erectile and associated adaptations to the Earth’s gravity in their physiological and pathological manifestations have not been considered. At the same time, it should be noted that humans, unlike other animal species, have the greatest vulnerability of the cardiovascular system (CVS). The latter is associated with fundamental differences in the functioning and regulation of the CVS by the influence of gravity on blood circulation in humans as upright creatures. Based on a review of comparative physiological, ontogenetic, and clinical studies from an evolutionary perspective, the idea of adaptation to the Earth’s gravity when walking upright in humans is justified as an anthropogenic basis for the physiology and pathology of the cardiovascular system and hemodynamic support systems (physio-anthropology and pathological anthropology).

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Lactate dehydrogenase activity, crystallogenic and initiator characteristics of biological substrates were evaluated by enzymological and crystalloscopic analysis of rat serum and urine. Changes in these characteristics of biological media in combined thermal injury were shown. This approach is effective for evaluation of the metabolic status in rats with experimental burn disease.