Estimation of human saliva structurization at dehydration on the solid substrate

Crystalloscopic analysis in detection of saliva metabolome specialties in elderly people

“…These changes indicate that metabolic rearrangements that occur during age-related changes lead not only to a quantitative decrease in the crystallogenic activity of the biological fluid, but also to a simplification of its formed structures. This indicates rather deep shifts in the physico-chemical properties of the biosubstrate [10,11].…”

“…Samples of mixed saliva were collected by spitting into clean, dry test tubes after twice rinsing the oral cavity with distilled water. The intrinsic crystallization of mixed saliva was studied using the method of classical crystalloscopy described earlier [10,11]. The structure index (SI), crystallizability (Cr), the facia destruction degree (FDD) and the clearity of the marginal protein zone (Mz) were determined.…”

“…The structure index (SI), crystallizability (Cr), the facia destruction degree (FDD) and the clearity of the marginal protein zone (Mz) were determined. Each of the parameters was expressed on a 5-point scale, according to the previously described criteria [10,11]. Since there is no data on the reference values of saliva crystalloscopy indicators in the elderly, in this study, the results in the elderly population were compared with the data obtained during the previous study in the middle age group (45-60 years).…”

“…Of particular interest is the assessment of the facia destruction degree -the main criterion for the "correctness" of crystallogenesis, currently considered as an indicator of the degree of changes in the crystallogenic properties of biological fluids [11]. It was found that in middle-aged adults, only moderate signs of destruction of facias elements were recorded in dried saliva samples, whereas in most elderly people, subtotal destruction of the crystal structures of the micropreparation was observed (Fig.…”

“…Biocrystallomics technologies are one of the methodological techniques that integrally describe the physical and chemical properties of biological fluids, in particular, mixed saliva [10,11]. It is known that the crystallogenic activity of biological media of an organism depends on their component composition and, therefore, allows us to indirectly assess the current state and dynamics of rearrangement of the metabolome of biological fluids in various functional states [11,12]. On this basis, it is logical to assume that the diagnostic technology under consideration will be informative in monitoring the involutive transformations of the composition of biological fluids [13].…”