Cold helium plasma as a modifier of free radical processes in the blood: in vitro study

Мартусевич, А. К.; Galka, A. G.; Karuzin, Konstantin A.; Tuzhilkin, Alexander N.; Malinovskaya, S. L.

doi:10.3934/biophy.2021002

Cited by 5 publications

(13 citation statements)

References 21 publications

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“…12,13,18,20,25 In our previous experiments using ironinduced biochemiluminescence, the severity of free radical processes and the state of the antioxidant system directly depended on the exposure time to cold plasma. 21 Taking this into account, we assume that under the used mode of application of the helium flow, oxidative stress is realized in the blood samples, which leads to the detected shifts in their crystallogenic properties. 26 A similar negative effect was observed for nonionized argon.…”

Section: Discussionmentioning

confidence: 99%

“…The cold plasma was generated using a device developed at the Institute of Applied Physics of the Russian Academy of Sciences (Nizhny Novgorod), which uses the principle of microwave ionization of the gas flow, previously described by us. 21 The temperature of the weakly ionized gas jet was 32°C, and the average power of the source did not exceed 20 W. The distance from the end of the plasma torch to the surface of the biological fluid was 1.0 to 1.5 cm. The duration of the treatment was 1 minute, and the exposure after its completion was 15 minutes.…”

Section: A Materials and Experiments Descriptionmentioning

confidence: 99%

“…Then we studied the intrinsic crystallogenic activity of blood plasma by classical crystalloscopy. [21][22][23] The description of dehydrated blood plasma samples, called facia, was performed morphologically and using a system of visuametric (visuametry is a method of morphometrically describing crystallization effects with visual noninstrumental criteria) parameters that characterize the qualitative and quantitative aspects of the process of crystallization of the biological medium. Main morphometric parameters for analysis of blood plasma crystallization include crystallizability, structure index, facia destruction degree, and size of marginal zone.…”

Section: Estimation Of Crystallogenic Properties Of Blood Samplesmentioning

confidence: 99%

“…Main morphometric parameters for analysis of blood plasma crystallization include crystallizability, structure index, facia destruction degree, and size of marginal zone. [21][22][23] All these parameters are calculated in direct four-points scale. Crystallizability is characterizing the density of crystals in dried specimens (defined as less than 10; 10 to 20; 20 to 30, and more than 30 crystal structures in the field of view for 0, 1, 2, and 3 points, respectively).…”

Section: Estimation Of Crystallogenic Properties Of Blood Samplesmentioning

confidence: 99%

“…26 In addition, we have previously demonstrated the dependence of metabolic shifts in the biological fluid on the time of exposure to cold plasma. 21 At the same time, the effect of the carrier gas on the modulating effect of cold plasma on the crystallization of blood serum has not been studied. Based on this, the aim of this study was to compare the biological effects of helium and argon plasma in vitro (for example, the effect on the crystallogenic properties of blood).…”

Section: Introductionmentioning

confidence: 99%

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Comparative Study of the Influence of Helium and Argon Plasma on Crystallogenic Properties of the Blood

et al. 2021

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The aim of this study was to compare the biological effects of helium and argon plasma in vitro. The cold plasma was generated using a device developed at the Institute of Applied Physics of the Russian Academy of Sciences (Nizhny Novgorod), which uses the principle of microwave ionization of the gas flow. We studied the crystallogenic activity of blood plasma. The description of dehydrated blood plasma samples was performed morphologically, using a system of visuametric parameters. Main morphometric parameters for analysis of blood plasma crystallization include crystallizability, structure index, facia destruction degree, and size of marginal zone. This study allowed us to establish that the effect of helium and argon in both free and ionized forms on the crystallogenic activity of blood varies significantly. The effect of cold plasma in general should be described as more favorable relative to the corresponding nonionized fluxes. At the same time, the most optimal nature of the reaction of the biological fluid was recorded when using a helium cold plasma. A specific feature of the helium flow is the suppression of the crystallogenic activity of the biological fluid, and the argon flow leads to its increase. Ionization of gases optimizes the nature of their influence, moreover, according to the modeling effect, the helium cold plasma is closest to the control sample.

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Section: Discussionmentioning

confidence: 99%

Section: A Materials and Experiments Descriptionmentioning

confidence: 99%

Section: Estimation Of Crystallogenic Properties Of Blood Samplesmentioning

confidence: 99%

Section: Estimation Of Crystallogenic Properties Of Blood Samplesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Study of the Influence of Helium and Argon Plasma on Crystallogenic Properties of the Blood

et al. 2021

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Experimental Evaluation of the Effect of Argon Cold Plasma on Oxidative Metabolism of the Blood

et al. 2022

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Some Beneficial Effects of Inert Gases on Blood Oxidative Metabolism: In Vivo Study

Мартусевич

Surovegina

Поповичева

et al. 2022

BioMed Research International

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The aim of the study was to assess the effect of external use of inert gases (helium and argon) on the state of free radical processes in vivo. The experiment was performed on 30 male Wistar stock rats (age-3 months, weight–200-220 g.), randomly distributed into 3 equal groups. The first group of animals was intact ( n = 10 ). The animals of the second and third groups were treated with argon and helium streams, respectively. Our research has allowed us to establish that the studied inert gases have a modulating effect on the state of oxidative metabolism of rat blood, and the nature of this effect is directly determined by the type of gas. The results of this study allowed us to establish the potential antioxidant effect of the helium stream, mainly realized due to the activation of the catalytic properties of the enzymatic link of the antioxidant system of rat blood plasma. At the same time, the revealed features of shifts in oxidative metabolism during treatment with argon flow include not only stimulation of the antioxidant system but also the pronounced induction of free radical oxidation. Thus, the conducted studies made it possible to verify the specificity of the response of the oxidative metabolism of blood plasma to the use of inert gases, depending on their type.

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Cold helium plasma as a modifier of free radical processes in the blood: in vitro study

Cited by 5 publications

References 21 publications

Comparative Study of the Influence of Helium and Argon Plasma on Crystallogenic Properties of the Blood

Comparative Study of the Influence of Helium and Argon Plasma on Crystallogenic Properties of the Blood

Experimental Evaluation of the Effect of Argon Cold Plasma on Oxidative Metabolism of the Blood

Some Beneficial Effects of Inert Gases on Blood Oxidative Metabolism: In Vivo Study

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