Some Beneficial Effects of Inert Gases on Blood Oxidative Metabolism: In Vivo Study

Abstract

The aim of the study was to assess the effect of external use of inert gases (helium and argon) on the state of free radical processes in vivo. The experiment was performed on 30 male Wistar stock rats (age-3 months, weight–200-220 g.), randomly distributed into 3 equal groups. The first group of animals was intact ( n = 10 ). The animals of the second and third groups were treated with argon and helium streams, respectively. Our research has allowed us to establish that the studied inert gases have a modulating effect on the state of oxidative metabolism of rat blood, and the nature of this effect is directly determined by the type of gas. The results of this study allowed us to establish the potential antioxidant effect of the helium stream, mainly realized due to the activation of the catalytic properties of the enzymatic link of the antioxidant system of rat blood plasma. At the same time, the revealed features of shifts in oxidative metabolism during treatment with argon flow include not only stimulation of the antioxidant system but also the pronounced induction of free radical oxidation. Thus, the conducted studies made it possible to verify the specificity of the response of the oxidative metabolism of blood plasma to the use of inert gases, depending on their type.

The Influence of Electromagnetic Radiation of the Ehf Band on Blood Oxidative Metabolism Under Modeling the Engraftment of a Skin Flap

“…Blood stabilized with sodium citrate (1:9) was used to study the balance of pro-and antioxidant systems. In plasma and suspensions of washed erythrocytes in saline solution (1:4), the activity of free radical oxidation processes was studied using method of Fe-induced biochemiluminescence [4] on a biochemiluminometer BHL-06 (Nizhny Novgorod). The level of malondialdehyde (MDA) in plasma and hemolysate of washed erythrocytes (1:10) was determined by the method of M. Uchiyama, M. Mihara [7].…”

“…The activity of SOD was determined in the hemolysate of washed erythrocytes (1:10) by inhibiting the formation of the product of autoxidation of adrenaline [4]. A spectrophotometric method was used to evaluate catalase activity in the hemolysate of washed erythrocytes (1:100).…”

“…The body has an antioxidant multicomponent system that protects cells and tissues from free radical oxidation. Its most important and effective link is a system of antioxidant enzymes that inhibit LPO at the stage of its initiation [4][5][6]. Thus, superoxide dismutase (SOD) inactivates the superoxide-anion radical; the substrates of catalase action are hydrogen peroxide and lipid hydroperoxides [6].…”

Are Protein Cavities and Pockets Commonly Used by Redox Active Signalling Molecules?

“…Other inert gases worth considering here are Neon (Ne) and helium (He). Both have reported effects [ 53 ]. However, just because one gas has an effect does not mean they all do.…”

Molecular Hydrogen: From Molecular Effects to Stem Cells Management and Tissue Regeneration

“…In parallel, ideas were developed about the biological properties of inert gases (helium, argon, xenon, etc.) which are chemically inactive under standard conditions, but show a modulating effect on cells and tissues [ 6 , 7 , 8 , 9 ]. These ideas served as the basis for the creation of innovative therapeutic technologies, the prospects of which continue to be studied and expanded upon.…”