Enzyme biosensor for androsterone based on 3α-hydroxysteroid dehydrogenase immobilized onto a carbon nanotubes/ionic liquid/NAD+ composite electrode

Single-walled carbon nanotubes covalently functionalized with polytyrosine: A new material for the development of NADH-based biosensors

“…The modification of electrodes with carbon nanomaterials (Gasnier et al, 2013;Sun et al, 2013;Wang et al, 2012), nanoparticles Jena and Raj, 2006;Sharifi et al, 2013) or hybrid nanomaterials (Agüí et al, 2009;Govindhan et al, 2015;Hosseini et al, 2014;Mundaca et al, 2012;Teymourian et al, 2013;Tiwari and Gupta, 2014) have demonstrated to be highly successful.…”

Expression, purification and functional characterization of a novel 3α-hydroxysteroid dehydrogenase from Pseudomonas aeruginosa

“…Besides its critical role in steroid metabolism pathways, 3a-HSDs also serve as a useful enzyme in the determination of 3a-hydroxysteroids and relevant compounds [6,[21][22][23]. Although 3a-HSDs have been found in both eukaryotic cells and prokaryotes [12,13], their biological sources are very limited [23].…”

Electrochemical biosensors for hormone analyses

“…For selectivity testing they used testosterone, β-estradiol, cortisol, 19-nortestosterone, uric and ascorbic acid as interferents. Cortisol and 19-nortestosterone, at concentrations ten times higher than respective physiological levels, testosterone, at concentrations 1000 times higher than its physical level were used and they found no significant response to their measurement (Mundaca et al, 2012). Progesterone (P4) is an unsaturated α, β ketone, and a steroid that is secreted by phase luteal ovarian cycle and during pregnancy.…”