Expression, purification and functional characterization of a novel 3α-hydroxysteroid dehydrogenase from Pseudomonas aeruginosa

Chen, Jianmin; Gao, Xiu-Feng; Hong, Lin; Ma, Liting; Li, Yongsheng

doi:10.1016/j.pep.2015.07.006

Cited by 6 publications

(2 citation statements)

References 25 publications

(40 reference statements)

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“…The formation of 3-keto intermediates in other bile acid-degrading bacteria suggests that this step is conserved in aerobic and anaerobic bile acids degradation [ 25 , 62 ]. 3 α -HSDs are also found in bacteria, which cannot utilize steroids as growth substrates, e.g., P. aeruginosa [ 62 , 63 ].…”

Section: Bacterial Bile Acid Degradationmentioning

confidence: 99%

Degradation of Bile Acids by Soil and Water Bacteria

et al. 2021

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Bile acids are surface-active steroid compounds with a C5 carboxylic side chain at the steroid nucleus. They are produced by vertebrates, mainly functioning as emulsifiers for lipophilic nutrients, as signaling compounds, and as an antimicrobial barrier in the duodenum. Upon excretion into soil and water, bile acids serve as carbon- and energy-rich growth substrates for diverse heterotrophic bacteria. Metabolic pathways for the degradation of bile acids are predominantly studied in individual strains of the genera Pseudomonas, Comamonas, Sphingobium, Azoarcus, and Rhodococcus. Bile acid degradation is initiated by oxidative reactions of the steroid skeleton at ring A and degradation of the carboxylic side chain before the steroid nucleus is broken down into central metabolic intermediates for biomass and energy production. This review summarizes the current biochemical and genetic knowledge on aerobic and anaerobic degradation of bile acids by soil and water bacteria. In addition, ecological and applied aspects are addressed, including resistance mechanisms against the toxic effects of bile acids.

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Section: Bacterial Bile Acid Degradationmentioning

confidence: 99%

Degradation of Bile Acids by Soil and Water Bacteria

et al. 2021

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“…Some other metal ions Mg 2+ , Ni 2+ and Al 3+ have no effect on 3α-HSDH. 30 Ch KRED20 one SDR, was cloned from Chryseobacterium sp. CA49 by Tang.…”

Section: Resultsmentioning

confidence: 99%

Co-immobilised 7α- and 7β-HSDH as recyclable biocatalyst: high-performance production of TUDCA from waste chicken bile

Wang

Chou

et al. 2018

RSC Adv.

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Chicken gallbladder has long been considered to be worthless and discarded as waste. The main composition of chicken bile is taurochenodeoxycholic acid (TCDCA), which is the isomeride of tauroursodeoxycholic acid (TUDCA). TUDCA has been effectively used for treatment of many diseases. In this paper, 7a-and 7bhydroxysteroid dehydrogenases (HSDH) were co-immobilised on modified chitosan microspheres, and used as recyclable biocatalyst for the catalysis of chicken bile. The catalytic reaction reached equilibrium within 4 h compared with 1 h using TCDCA as substrate. After four continuous batch reactions, the conversion of TCDCA was lower than 40% and TUDCA yield was about 15% for the catalysis of chicken bile. TUDCA yield was approximately 62% after equilibrium and the content of TUDCA in reaction product was as high as 33.16%. Furthermore, the experiments showed that activity of enzymes were significantly inhibited by bilirubin, Cu 2+ and Ca 2+ in complex substrate. The research described not only widens the utilization of chicken bile, but also provides a clean way for the preparation of TUDCA.

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