Enzyme Assay, Quantitative Determination of Peroxidase in Sweet Corn

Vetter, James L.; Steinberg, M. P.; Nelson, A. I.

doi:10.1021/jf60083a006

Cited by 133 publications

(65 citation statements)

References 1 publication

(1 reference statement)

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“…The peroxidase (POD) activity was measured by the strategy for Vetter et al (1958) as altered by Gorin and Heidema (1976). The test blend contained 50 µL of sample, 675 µL of 100 mM MES buffer (pH 5.5), 300 µL of 0.05% H 2 O 2 and 100 µL of 0.1% phenylenediamine.…”

Section: Peroxidase Activitymentioning

confidence: 99%

l-tryptophan-assisted PGPR-mediated induction of drought tolerance in maize (Zea maysL.)

Yasmin

Nosheen

Naz

et al. 2017

Journal of Plant Interactions

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Drought is an important abiotic stress that limits the plant growth and productivity. Present investigation was aimed that plant growth-promoting rhizobacteria (PGPR) isolated from moisturestressed area impart drought tolerance in plants and tryptophan may improve their efficiency. Pseudomonas sp. (1), Bacillus cereus and Bacillus pumilus (B. pumilus) were isolated from maize rhizosphere grown in irrigated fields, semi-arid region and arid region, respectively. Proteus sp. and Pseudomonas sp. (2) were isolated from rice rhizosphere grown in irrigated fields and raised bed. B. pumilus produced 5× more abscisic acid (ABA) in culture media than Pseudomonas sp. (1) by the addition of L-tryptophan. These inoculants also modulated the phytohormone content of maize leaves in a pot experiment. Higher ABA was produced by the application of B. pumilus and Pseudomonas sp. (2), while indole 3-acetic acid and gibberellic acid were found higher in Pseudomonas sp. (1) and Proteus sp. treated plants. Addition of L-tryptophan increased the concentration of all phytohormones in soil and leaves of maize. Maximum increase in relative water content, osmotic potential, protein content and photosynthetic pigments was recorded in B. pumilus treated maize plants. Under irrigated condition, response of Pseudomonas sp. coinoculated with B. pumilus from arid field superseded while under drought stress the effect of later predominated. Bacillus pumilus can be used in the formulation of biofertilizer to alleviate drought stress in arid and semi-arid regions. ARTICLE HISTORY

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Section: Peroxidase Activitymentioning

confidence: 99%

l-tryptophan-assisted PGPR-mediated induction of drought tolerance in maize (Zea maysL.)

Yasmin

Nosheen

Naz

et al. 2017

Journal of Plant Interactions

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“…The supernatants were kept in deep freezer till use. Peroxidase activity was measured according to Vetter et al (1958). In 200 µL of sample, the following reagent (1 mL of 1% ophenylenediamine in 95% ethyl alcohol, fresh every four hours) plus 1 mL of 0.3% hydrogen peroxide was added.…”

Section: Determination Of Peroxidase Activitymentioning

confidence: 99%

Role of Jasmonic Acid and Gamma Radiation in Alleviating Salt Stress in Moringa

Hamideldin¹,

Eliwa²,

Hussein³

2017

IJAB

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To cite this paper: Hamideldin, N., N. Eid Eliwa and O.S. Hussein, 2017. Role of jasmonic acid and gamma radiation in alleviating salt stress in moringa. AbstractThe present investigation studied the effect of two doses of γ-rays (20, 40 Gy) or foliar spraying of jasmonic acid 0.2 and 0.3 µM on moringa plants under salt stress condition (4000 mg/L NaCl). Foliar spray by jasmonic acid especially (0.3 µM) gave the highest peroxidase and polyphenol oxidase enzymes activity under normal or stress condition. Gamma rays and jasmonic acid treatments caused changes in activities, the band concentration of peroxidase and polyphenol oxidase isozymes and increased amino acid contents. Under salt stress condition, Cl, Fe, K and Na percentage increased significantly and of Ca, Mg and S decreased. The greatest level of K were also observed in samples treated by γ-rays or sprayed by JA under normal or salt stress condition. Gamma irradiation with dose 20 Gy and spraying with jasmonic acid (0.3 µM) increased moringa resistance to salt stress during growth under 4000 mg/L level of salt stress.

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“…The method of Beauchamp and Frodovich (1971) was used for superoxide dismutase (SOD) estimation. Estimation of peroxidase (POD) enzyme was done by the method of Vetter et al (1958) and modified by Gorin and Heidema (1976). CAT activity was estimated by the method of Chandlee and Scandalios (1984).…”

Section: Determination Of Effects Of Pgpr and Their Eps On Physiologimentioning

confidence: 99%

Role of plant growth-promoting rhizobacteria and their exopolysaccharide in drought tolerance of maize

Naseem

Bano

2014

Journal of Plant Interactions

330

128

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The present study deals with the isolation and characterization of exopolysaccharides (EPS) produced by the plant growth-promoting rhizobacteria (PGPR) from arid and semiarid regions of Pakistan, and to investigate the drought tolerance potential of these PGPR on maize when used as bioinoculant alone and in combination with their respective EPS. Three bacterial strains Proteus penneri (Pp1), Pseudomonas aeruginosa (Pa2), and Alcaligenes faecalis (AF3) were selected as EPS-producing bacteria on the basis of mucoid colony formation. All these strains were gram negative, motile, and positive for catalase. Strain Pp1 was positive for oxidase test and was phosphate solubilizing, while Pa2 and AF3 were negative. The isolated strains were sequenced using 16SrRNA. Total soluble sugar, protein, uronic acid, emulsification activity, and Fourier-transformed infrared spectroscopy of EPS were determined. Drought stress had significant adverse effects on growth of maize seedlings. Seed bacterization of maize with EPS-producing bacterial strains in combination with their respective EPS improved soil moisture contents, plant biomass, root and shoot length, and leaf area. Under drought stress, the inoculated plants showed increase in relative water content, protein, and sugar though the proline content and the activities of antioxidant enzymes were decreased. The Pa2 strain isolated from semiarid region was most potent PGPR under drought stress. Consortia of inocula and their respective EPS showed greater potential to drought tolerance compared to PGPR inocula used alone.

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Enzyme Assay, Quantitative Determination of Peroxidase in Sweet Corn

Cited by 133 publications

References 1 publication

l-tryptophan-assisted PGPR-mediated induction of drought tolerance in maize (Zea maysL.)

l-tryptophan-assisted PGPR-mediated induction of drought tolerance in maize (Zea maysL.)

Role of Jasmonic Acid and Gamma Radiation in Alleviating Salt Stress in Moringa

Role of plant growth-promoting rhizobacteria and their exopolysaccharide in drought tolerance of maize

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