Enzyme amperometric sensor for the determination of cholinesterase inhibitors or activators

Babkina, S. S.; Medyantseva, É. P.; Budnikov, H. C.; Винтер, В. Г.

doi:10.1016/0003-2670(93)80185-n

Cited by 10 publications

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“…[223][224][225] MFE's have been used in hyphenated electrochemicalspectroscopic methods as preconcentrating devices for atomic spectroscopy [226][227][228][229][230] and chemiluminescence 231 as well as in thin-layer chromatography-stripping voltammetry 232,233 and anion exchange-stripping voltammetry. 234 Kinetic studies of enzyme inactivation, 235,236 the effect of metals on enzyme activity 237 and kinetic separations 238 on MFE's have been reported. MFE's have been applied to the indirect determination of surfactants, 239,240 EDTA, 241 oxygen 242 and thorium and scandium.…”

Section: Selected Applications Of Mfe'smentioning

confidence: 99%

Mercury film electrodes: developments, trends and potentialities for electroanalysis

Economou

Fielden²

2003

Analyst

120

128

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Section: Selected Applications Of Mfe'smentioning

confidence: 99%

Mercury film electrodes: developments, trends and potentialities for electroanalysis

Economou

Fielden²

2003

Analyst

120

128

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“…Moreover, the use of electrochemistry makes it possible for us to draw some conclusions regarding the mechanism of macromolecule-involving processes, particularly regarding DNA behavior both in solution and at the surface of the electrode. The latter simulates the properties of DNA in a cell membrane. , In ref , it was suggested to employ enzyme modulator-mediated immunoassay involving a cholinesterase (ChE)-based amperometric electrode for the determination of antibodies to DNA and AMD diagnosis, which takes the autoimmune origin of the disease into consideration, with organophosphorus inhibitors covalently bound to DNA used as modulators.…”

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New Variants of Enzyme Immunoassay of Antibodies to DNA

Medyantseva

et al. 1996

Anal. Chem.

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A method of DNA immobilization on cellulose nitrate films has been developed. Modified films of uniform and stable surface have been used to devise two variants of solid-phase enzyme immunoassays of antibodies. The co-immobilization of enzyme label (cholinesterase) and the DNA molecules makes it possible to carry out the procedure of solid-phase enzyme immunoassay without any separation of components. Thus, it takes only 15 min to diagnose an autoimmune disease (Aleutian disease of minks) with the immunoenzyme amperometric sensor, with a lower detection limit for antibodies of 0.5 x 10(-10) M. For scaled diagnosing, solid-phase enzyme immunoassay on DNA-modified films with prior separation of components and spectrophotometric registration of peroxidase activity has been developed. The time for determination was 30 min, with a lower detection limit of 7.4 x 10(-12) M.

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Electrochemical Immunoassay

Wijayawardhana

Halsall

Heineman

2002

Encyclopedia of Electrochemistry

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The sections in this article are Introduction Enzyme Electrochemical Immunoassays Electrochemical Detection The E ‐ S ‐ P System Heterogeneous Enzyme Immunoassay Ultrasensitive Heterogeneous Enzyme Assays in Small Volumes Homogeneous Enzyme Immunoassay Nonenzyme Electrochemical Immunoassays Nonenzyme Homogeneous Immunoassays Heterogeneous Nonenzymatic Immunoassays Electrochemical Immunosensors Applications Conclusion

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Enzyme amperometric sensor for the determination of cholinesterase inhibitors or activators

Cited by 10 publications

References 10 publications

Mercury film electrodes: developments, trends and potentialities for electroanalysis

Mercury film electrodes: developments, trends and potentialities for electroanalysis

New Variants of Enzyme Immunoassay of Antibodies to DNA

Electrochemical Immunoassay

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