New Variants of Enzyme Immunoassay of Antibodies to DNA

Ss, Babkina; Medyantseva, E. A.; Hc, Budnikov; Mp, Tyshlek

doi:10.1021/ac950645v

Cited by 35 publications

(18 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, it provides a route for rapid screening of these compounds. Some analytical applications based on the interaction of DNA with chemicals include the detection of hydrazines [23], nitroimidazoles [24], mifepristone [25], mitoxantrone [26], carboplatin [27], carcinogens [28], aromatic amines [29], and the detection of compounds of clinical significance that may lead to the rapid detection of AIDS [30,31].…”

Section: Introductionmentioning

confidence: 99%

Determination of Cytochrome C with Cellulose – DNA Modified Carbon Paste Electrodes

et al. 2004

View full text Add to dashboard Cite

A sensor for cytochrome c (cyt c) was developed using a carbon paste electrode (CPE) modified with cellulose-DNA. Cyt c was adsorbed on the cellulose-DNA modified CPE through the electrostatic interaction between them. Owing to this process, a pair of well-defined peaks appeared at 48 mV/85 mV (E pc /E pa vs. Ag/AgCl). This property of the cellulose-DNA modified CPE was utilized for the analysis of cyt c in a biological sample. The optimum experimental conditions for analysis were investigated and a calibration plot was obtained between 1.0 Â 10 À4 M and 1.0 Â 10 À6 M (AE 5% at n 5) at the optimized condition. The detection limit for cyt c at the optimized experimental condition was 5.0 Â 10 À7 M (S/N 3) for 30 min of deposition time with differential pulse voltammetry (DPV). The real sample analysis was carried out with the standard addition method to evaluate the developed method. The content of cyt c in total proteins of 80.0 mg/mL in rat mitocondria fractions was determined to be 0.12 (AE 0.02) mg/mL.

show abstract

Section: Introductionmentioning

confidence: 99%

Determination of Cytochrome C with Cellulose – DNA Modified Carbon Paste Electrodes

et al. 2004

View full text Add to dashboard Cite

show abstract

“…Low detection limits and low time consumed can be achieved with modern electrochemical techniques. One approach used in such techniques is to employ an enzyme label that generates an electrochemically active product (Babkina et al, 1996;Parellada et al, 1998;Duane and Meyerhoff, 1994;Manning et al, 1994;Du et al, 2005;Messina et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Integrated microfluidic systems with an immunosensor modified with carbon nanotubes for detection of prostate specific antigen (PSA) in human serum samples

Panini

Messina

Salinas

et al. 2008

Biosensors and Bioelectronics

115

View full text Add to dashboard Cite

“…The binding of specific antibodies resulted in the decrease of the rate of enzymatic reaction due to the suppression of the substrate delivery from the bulk solution. The apparent inhibition of enzyme activity was recorded amperometrically in DC mode [18,19]. The appropriate DNA-sensors were successfully applied for diagnostics of human systemic lupus erythemathosus and Aleutian disease of mink.…”

Section: Introductionmentioning

confidence: 99%

Potentiometric DNA Sensor Based on Electropolymerized Phenothiazines for Protein Detection

et al. 2008

View full text Add to dashboard Cite

Novel method of potentiometric detection of DNA-protein interactions has been proposed. For this purpose, polymeric phenothiazine dyes, methylene blue (MB) and methylene green (MG), were electrochemically deposited onto the glassy carbon electrode and covered with double stranded DNA (dsDNA) as a target for antibodies (DNAsensor) or DNA aptamer specific to human a-thrombin (aptasensor). The biosensors were consecutively incubated at pH 7.5 and 3.0 and the difference in potentials, DE, was used as a measure of protein concentration. The potentiometric DNA-sensors were tested in standard serum of autoimmune disease patients (systemic lupus erythemathosus (SLE) and autoimmune thyroidites). It was shown, that the DE value of DNA-sensor depends on the dilution of serum in the range from 1 : 1 to 1 : 100. Nonthermostated serum exhibited bell-shape dependence of DE on serum dilution due to interfering effect of serine proteins at maximum dilution between 1 : 20 and 1 : 50. For SLE serum thermostated at 56 8C the DE linearly decreased as a function of serum dilution and reached saturation at dilution 1 : 20. Similarly the changes in the potential of aptasensor allowed us to determine the a-thrombin in the range from 1 nM to 1 mM. The Faradic impedance spectra measured at presence of redox probe [Fe(CN) 6 ] 4À/3À revealed changes in the resistance and capacitance attributed to the shielding effect of anti-DNA antibodies and an increase in the electron transfer. The developed potentiometric biosensors can be used for preliminary diagnostics of autoimmune diseases and thrombin detection with sensitivity comparable to traditional methods. The developed assay is, however simpler and cheaper in comparison with commonly used methods.

show abstract

New Variants of Enzyme Immunoassay of Antibodies to DNA

Cited by 35 publications

References 15 publications

Determination of Cytochrome C with Cellulose – DNA Modified Carbon Paste Electrodes

Determination of Cytochrome C with Cellulose – DNA Modified Carbon Paste Electrodes

Integrated microfluidic systems with an immunosensor modified with carbon nanotubes for detection of prostate specific antigen (PSA) in human serum samples

Potentiometric DNA Sensor Based on Electropolymerized Phenothiazines for Protein Detection

Contact Info

Product

Resources

About