Enzymatic synthesis optimization of isoamyl butyrate

Abstract

Lipozyme TL IM foi usada para catalisar a esterificação de álcool isoamílico e ácido butírico. Um planejamento fatorial fracionário foi utilizado para avaliar os efeitos da temperatura (30, 40, 50 ºC), razão molar álcool:ácido (1:1, 2:1, 3:1), concentração de enzima (0,003, 0,0115, 0,020 g mL -1 ), concentração de ácido butírico (0,1, 0,3, 0,5 mol L -1 ) e agitação (50, 115, 180 rpm) na produção de éster. Com esses resultados, os níveis foram redefinidos para um planejamento fatorial 2³. A máxima produção de éster foi obtida a 30 ºC, 180 rpm, razão molar álcool:ácido de 1:1, concentração de enzima 0,021 g mL -1 e concentração de ácido butírico de 0,5 mol L -1 . Sob essas condições ótimas foi obtido 92% de esterificação e concentração de éster de 0,9 mol L -1 . Álcool isoamílico obtido a partir de óleo fúsel foi utilizado nas mesmas condições resultando em 93% de esterificação e concentração de éster de 1,0 mol L -1 .Lipozyme TL IM was used to catalyse the esterification of isoamyl alcohol and butyric acid. A fractional factorial design was employed to evaluate the effects of temperature (30, 40, 50 °C), alcohol:acid molar ratio (1:1, 2:1, 3:1), enzyme concentration (0.003, 0.0115, 0.020 g mL -1 ), butyric acid concentration (0.1, 0.3, 0.5 mol L -1 ) and shaking rate (50, 115, 180 rpm) on the ester yield. With these results, the levels were redefined to a 2 3 factorial design. The maximum yield of ester was obtained at 30 ºC, 180 rpm, alcohol:acid molar ratio of 1:1, enzyme concentration of 0.021 g mL -1 and butyric acid concentration of 0.5 mol L -1 . Under the optimal conditions, 92% esterification was attained with an ester concentration of 0.9 mol L -1 . Isoamyl alcohol from fusel oil was used under the same conditions and resulted in 93% esterification and an ester concentration of 1.0 mol L -1 .

Optimized butyl butyrate synthesis catalyzed by Thermomyces lanuginosus lipase

“…Lipase from T. lanuginosus has been mainly reported for reactions with long chain fatty acids, and transesterification with oils, with very few works found for esterification between short chain acids and alcohol. Among these works, yields comparable to those obtained in this work were reached only after much longer reaction times (18 h or more) . Therefore, the results presented in this article represent an improvement in the process productivity.…”

Optimization of agroindustrial medium for the production of carotenoids by wild yeast Sporidiobolus pararoseus

“…The composition of the production medium using S. salmonicolor (Valduga et al, 2009(Valduga et al, , 2014 and for S. pararoseus (Cabral et al, 2011). This methodology is also applied to obtain other bioproducts, such as biomass (Santos et al, 2012), galacto-oligosaccharides (Lisboa et al, 2012a;Lisboa et al, 2012b), dairy drinks (Martins et al, 2011;Martins et al, 2012;Burkert et al, 2012), enzymes (Maldonado et al, 2012;Campello et al, 2012;Alves et al, 2010), synthesis of natural flavors (Anschau et al, 2011;Aragão et al, 2011), phycocyanin (Moraes et al, 2010) and rhamnolipids (Rosa et al, 2010).…”

Solvent free lipase catalyzed synthesis of butyl caprylate

“…4 Numerous valuable esters have been successfully synthesized in presence of lipases as biocatalyst. 3,5,6 Lipases, Triacylglycerol hydrolases [E.C 3.1.1.3] are produced from many bacterial and fungal sources. As enzymes are produced from natural sources their cost of production and related treatments further make the enzymes costly.…”