Enzymatic production of methyl esters from low-cost feedstocks

Mibielli, Guilherme Martinez; Fagundes, Ana Paula; Bohn, Letícia Renata; Cavali, Matheus; Bueno, Auriane; Bender, João Paulo; Oliveira, J. Vladimir

doi:10.1016/j.bcab.2020.101558

Cited by 21 publications

(7 citation statements)

References 40 publications

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“…In general, even using raw material with different acidities, the Eversa performance was very close. In relation to the initial saponifiable matter (91-96 wt.% saponification index), the conversion of saponifiable matter to FAEEs was up to around 90%, showing the good performance of this enzyme for lowly and highly-acid raw materials, as already demonstrated with other fatty materials [1,4,7,8,10,11,13,14,19,55,[57][58][59]. For example, Miranda et al, [5] reported FAEE yields around 90 wt.% after 48 h of reaction in the presence of 6.0% water, using liquid Eversa and refined soybean oil.…”

Section: Performance Of Liquid Eversa In Simultaneous Transesterifica...supporting

confidence: 64%

“…As expected, both enzyme concentration (more expressive) and molar ratio (ethanol:SODD-SP) influenced positively the ester yield, i.e., an increase in these parameters leads to higher ester yields. In particular, as SODD has a high content of FFAs and its esterification generates water as a by-product, the reaction was favored using an excess of ethanol, which shifts the reaction equilibrium towards the products [17,55]. On the other hand, temperature negatively affected the ester yield, i.e., the reaction was favored at lower temperatures because high temperatures can inactivate the enzyme.…”

Section: Assaysmentioning

confidence: 99%

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Performance of Liquid Eversa on Fatty Acid Ethyl Esters Production by Simultaneous Esterification/Transesterification of Low-to-High Acidity Feedstocks

et al. 2021

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Liquid Eversa was evaluated in hydrolysis of acylglycerols from soybean oil deodorizer distillate (SODD), as well as simultaneous esterification/transesterification of SODD with low-to-high free fatty acids (FFAs) content using ethanol as acyl acceptor. Hydrolysis of SODD at mild temperature (37 °C) and without pH control (water:SODD mass ratio of 4:1) increased its FFAs content from 17.2 wt.% to 72.5 wt.% after 48 h reaction. A cold saponification of SODD allowed a saponification phase (SODD-SP) to be recovered with 93 wt.% saponification index and 2.25 wt.% FFAs content, which was used to find the experimental conditions for simultaneous esterification/transesterification reactions by experimental design. Temperature of 35 °C, enzyme concentration of 8.36 wt.%, and molar ratio of 3.64:1 (ethanol:SODD-SP) were found as the best conditions for fatty acid ethyl esters (FAEEs) production from SODD-SP (86.56 wt.% ester yield after 23 h reaction). Under the same reaction conditions, crude SODD (17.2 wt.% FFAs) and hydrolyzed SODD (72.5 wt.% FFAs) yielded products containing around 80 wt.% FAEEs. Caustic treatment could increase the ester content to around 90 wt.% and reduce the FFAs content to less than 1 wt.%. Our results show the good performance of liquid Eversa in aqueous (hydrolysis reactions) and organic (esterification/transesterification reactions) media.

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Section: Performance Of Liquid Eversa In Simultaneous Transesterifica...supporting

confidence: 64%

Section: Assaysmentioning

confidence: 99%

Performance of Liquid Eversa on Fatty Acid Ethyl Esters Production by Simultaneous Esterification/Transesterification of Low-to-High Acidity Feedstocks

et al. 2021

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“…It is worth noting that Brazil is the world leader in the use of energy from renewable sources; the most widely used raw material is sugarcane juice, characterizing the country as the largest ethanol producer from that feedstock (Mączyńska et al, 2019). However, despite being one of the biggest producers, Brazil still needs to diversify the productive matrix in order to develop production routes from different raw materials Mibielli et al, 2020). Accordingly, since there is a growing demand for food and energy, lignocellulosic materials become a viable option for ethanol production (Talebnia et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Alkaline pretreatment and enzymatic hydrolysis of corn stover for bioethanol production

Bohn

Dresch

Cavali

et al. 2021

RSD

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The demand for ethanol in Brazil is growing. However, although the country is one of the largest producers of this fuel, it is still necessary to diversify the production matrix. In that regard, studies with different raw materials are needed, mainly the use of low cost and high available wastes such as lignocellulosic residues from agriculture. Therefore, this study aimed to analyze the bioethanol production from corn stover. An alkaline pretreatment (CaO) was carried out, followed by enzymatic hydrolysis (Cellic Ctec2 and Cellic Htec2) to obtain fermentable sugars. The best experimental condition for the pretreatment and hydrolysis steps resulted in a solution with 0.31 gsugar∙gbiomass-1. Then, the fermentation was performed by the industrial strain of Saccharomyces cerevisiae (PE-2) and by the wild yeast strain Wickerhamomyces sp. (UFFS-CE-3.1.2). The yield obtained was 0.38 gethanol∙gdry biomass-1 was, demonstrating the potential of this process for bioethanol production.

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“…These include weak catalytic activity, catalytic site leaching, 18,19 and microporosity 9,14 . Studies 20‐23 reveal that enzyme‐based catalysts have several advantages over other catalysts, they achieve adequate yields, the biodiesel obtained is of higher quality, and it is an environmentally friendly process. Nevertheless, the use of enzyme catalysts has significant disadvantages such as denaturation of the enzyme, high sensitivity to alcohol, longer reaction time required, and higher costs 9,24‐29 …”

Section: Introductionmentioning

confidence: 99%

Biomass‐based heterogeneous catalysts for biodiesel production: A comprehensive review

Tobío‐Pérez

Domínguez

Rodríguez-Machín

et al. 2021

Intl J of Energy Research

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Summary Biodiesel is one of the most widely used alternative fuels to reduce exhaust emissions and the use of conventional fossil fuels. It can be synthesized from a transesterification reaction from vegetable oils or animal fats in the presence of homogeneous or heterogeneous catalysts. Some drawbacks of using homogeneous catalysts increased attention to heterogeneous catalysts for biodiesel production. Recently, heterogeneous catalysts derived from biomass have risen to the forefront of biodiesel production due to their sustainability, economical and eco‐friendly nature. In addition, they are easily recovered and constitute an alternative to eliminate biomass residues. This review highlights several biomass sources used as precursors for the production of heterogeneous catalyst. Furthermore, methods for preparing heterogeneous catalysts, the reaction mechanisms, catalyst advantages and drawbacks, their performance in biodiesel production, as well as the methodologies developed for their effective recovery are discussed in detail. Among lignocellulosic biomass‐based precursors, the paper takes into account those based on biochar, ash, carbonaceous substrate, and seed oil cake. Those catalysts obtained by both preparation methods (calcination and activation) have good catalytic activity for waste cooking oil or neat oils. Biomass ash or biochar‐based catalysts are also promising routes in biodiesel synthesis, but significant reductions in catalyst load, reaction time, temperature, and methanol‐to‐oil ratio must be reached.

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Enzymatic production of methyl esters from low-cost feedstocks

Cited by 21 publications

References 40 publications

Performance of Liquid Eversa on Fatty Acid Ethyl Esters Production by Simultaneous Esterification/Transesterification of Low-to-High Acidity Feedstocks

Performance of Liquid Eversa on Fatty Acid Ethyl Esters Production by Simultaneous Esterification/Transesterification of Low-to-High Acidity Feedstocks

Alkaline pretreatment and enzymatic hydrolysis of corn stover for bioethanol production

Biomass‐based heterogeneous catalysts for biodiesel production: A comprehensive review

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