Alkaline pretreatment and enzymatic hydrolysis of corn stover for bioethanol production

Abstract

The demand for ethanol in Brazil is growing. However, although the country is one of the largest producers of this fuel, it is still necessary to diversify the production matrix. In that regard, studies with different raw materials are needed, mainly the use of low cost and high available wastes such as lignocellulosic residues from agriculture. Therefore, this study aimed to analyze the bioethanol production from corn stover. An alkaline pretreatment (CaO) was carried out, followed by enzymatic hydrolysis (Cellic Ctec2 and Cellic Htec2) to obtain fermentable sugars. The best experimental condition for the pretreatment and hydrolysis steps resulted in a solution with 0.31 gsugar∙gbiomass-1. Then, the fermentation was performed by the industrial strain of Saccharomyces cerevisiae (PE-2) and by the wild yeast strain Wickerhamomyces sp. (UFFS-CE-3.1.2). The yield obtained was 0.38 gethanol∙gdry biomass-1 was, demonstrating the potential of this process for bioethanol production.

Cellulases production from Aspergillus niger-ITV-02 using corn lignocellulosic residues

“…The structural composition of the crude residues reported in Table 1 is in the range of the reported in the literature for corn cob, cellulose 20-36 %, hemicellulose 33-42 %, and lignin 15-23 % approximately, concerning the corn stover cellulose 24-40 %, hemicellulose 30-45 % and lignin 20-25 % (Hernández et al, 2019;Rofiqah et al, 2019;Mafa et al, 2020;Bohn et al, 2021). Regarding delignification, the results obtained in this work are similar to the structural composition for corn cob and corn stover in reported previous research (Chen et al, 2013;Rofiqah et al, 2019).…”

Xylose Metabolization by a Saccharomyces cerevisiae Strain Isolated in Colombia

“…For strain 202-3, fermentations with the Hydrolysate-b showed ethanol production with a yield of 0.513 g ethanol‧ g − 1 sugar , even better than other industrial strains such as PE-2 that used corn stover hydrolysates with a concentration of 8.23 gL 1 of glucose obtaining a yield of 0.38 g ethanol‧ g − 1 sugar [ 21 ].…”