Enteractinococcus coprophilus gen. nov., sp. nov., of the family Micrococcaceae , isolated from Panthera tigris amoyensis faeces, and transfer of Yaniella fodinae Dhanjal et al. 2011 to the genus Enteractinococcus as Enteractinococcus fodinae comb. nov.

Abstract

A novel actinobacterium, designated strain YIM 100590T, was isolated from Panthera tigris amoyensis faeces collected from Yunnan Wild Animal Park in Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequence data showed that strain YIM 100590T is a member of the family Micrococcaceae . Cells were coccoid to oval (0.7–1.5 µm in diameter) occurring singly or in clusters. Growth was observed at 10–37 °C (optimum 28 °C) and at pH 7.0–11.0 (optimum pH 8.0). The major fatty acids were iso-C15 : 0 (32.22 %), anteiso-C15 : 0 (31.64 %) and iso-C16 : 0 (17.38 %). The peptidoglycan was of A4α type (l-Lys–Gly–l-Glu). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, dimannosyl diacylglycerol, an unknown glycolipid and two unknown phospholipids. The quinone system comprised menaquinones MK-7 (91.9 %) and MK-8 (8.3 %). The DNA G+C content of strain YIM 100590T was 56.2 mol%. Chemotaxonomic data indicated that the strain belongs to the family Micrococcaceae . On the basis of morphological and chemotaxonomic data and phylogenetic analysis, strain YIM 100590T is considered to represent a novel species of a new genus within the family Micrococcaceae , for which the name Enteractinococcus coprophilus gen. nov., sp. nov. is proposed. The type strain of Enteractinococcus coprophilus is YIM 100590T ( = DSM 24083T = JCM 17352T). Yaniella fodinae DSM 22966T was transferred to the new genus as Enteractinococcus fodinae comb. nov. (type strain G5T = DSM 22966T = JCM 17931T = MTCC 9846T).

Diversity and Bioactivity of Cultivable Animal Fecal Actinobacteria

“…A strain (YIM 100708) of Jiangella [32], a genus wildly distributing in saline and alkaline soil, desert, wall material of an indoor environment, cave and plant stem, were isolated from feces of Rhinopithecus bieti. Members of the genus Enteractinococcus, a novel genus, belong to Micrococcaceae, were isolated and characterized from three species of tigers [33]. It is worth to show that the 16S rDNA sequence similarities of 32 of 528 sequenced strains with valid published species were below 98.5%.…”

Review of the taxonomy of the genus Arthrobacter, emendation of the genus Arthrobacter sensu lato, proposal to reclassify selected species of the genus Arthrobacter in the novel genera Glutamicibacter gen. nov., Paeniglutamicibacter gen. nov., Pseudoglutamicibacter gen. nov., Paenarthrobacter gen. nov. and Pseudarthrobacter gen. nov., and emended description of Arthrobacter roseus

“…The details were already discussed above and are summarized below. The polar lipid profiles reported for the majority of arthrobacters and other members of the family Micrococcaceae including representatives of the genera Auritidibacter, Citricoccus, Enteractinococcus, Kocuria, Micrococcus, Nesterenkonia, Rothia, Sinomonas, Yaniella and Zhihengliuella (Embley et al, 1984;Kovács et al, 1999;Altenburger et al, 2002;Wieser et al, 2002;Li et al, 2004aLi et al, , 2005bZhang et al, 2007;Yassin et al, 2011a;Zhou et al, 2012;Cao et al, 2012) A. phenanthrenivorans, A. antarcticus, A. flavus and A. roseus were reported to contain PE and to lack any glycolipid. The close relatives of A. phenanthrenivorans (A. oxydans and A. polychromogenes), A. antarcticus (A. sulfureus) and A. flavus (A. agilis, A. parietis, A. tecti and A. tumbae) were shown to lack PE, and most of them have been shown to contain at least one glycolipid (Amadi & Alderson, 1982;Collins & Kroppenstedt, 1983;Heyrman et al, 2005; Fig.…”

Production of Bioagent for Calcium-Based Biocement

“…YIM 100590 was considered representing a novel species of a new genus within the family Micrococcaceae, for which the name Enteractinococcus coprophilus gen. nov., sp. nov. was proposed [32]. This strain was alkalophilic (was able to grow at pH 7.0-11.0 with the optimal pH 8.0), and halotolerant (tolerated up to 11 % (w/v) NaCl).…”