Enhancing Tissue Adhesion and Osteoblastic Differentiation of MC3T3‐E1 Cells on Poly(aryl ether ketone) by Chemically Anchored Hydroxyapatite Nanocomposite Hydrogel Coating

Liu, Chengde; Pan, Liang; Cheng, Liang; Li, Wentao; Li, Yizheng; Cheng, Xitong; Jian, Xigao

doi:10.1002/mabi.202100078

Cited by 12 publications

(7 citation statements)

References 59 publications

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“…The BPN-DFO hydrogel shows a faster degradation rate than the hydrogel, and the possible cause is that BPN can improve the hydrophilicity of the components. 44 …”

Section: Discussionmentioning

confidence: 99%

A Composite Deferoxamine/Black Phosphorus Nanosheet/Gelatin Hydrogel Scaffold for Ischemic Tibial Bone Repair

Xu¹,

Gan²,

Wang³

et al. 2022

IJN

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Introduction Bone delay union is mostly caused by lack of blood supply. Although autografts, allografts and artificial bone have been widely used to treat bone delay union, the bone regeneration fails in the ischemic site accompanied by the bone donor site complications and disease transmission. Recently, there is a growing recognition of the importance of hydrogel scaffolds which are regarded as an eligible engineer tissue for bone repair. However, hydrogel is still limited in improving neovascularization. Methods In this work, black phosphorus nanosheet and deferoxamine (BPN-DFO) were loaded in the gelatin hydrogel to overcome the high risk of bone delay union and systemically investigated the regeneration capability of BPN-DFO hydrogel in vitro and vivo. Results The resulting BPN-DFO hydrogel scaffold showed superior swollen, degradation and release rate, as well as satisfied biocompatibility. BPN-DFO hydrogel shown the significant up-expression of mRNA related to bone regeneration and cell proliferation. In vivo, the proposed BPN-DFO hydrogel significantly improved osteogenesis and neovascularization in the ischemic tibial bone site of SD rats with acute femoral artery occlusion. Both macroscopic and histological evaluation of new regenerated bone showed newly formed blood vessel and collagen using BPN-DFO hydrogel. The immunohistochemistry and RT-PCR revealed that the bone regeneration could be improved via BMP/Runx2 pathway. Conclusion The BPN-DFO hydrogel possesses potential tissue engineer material for ischemic bone defect treatment. However, furthermore studies are needed to testify the safety and efficacy of BPN-DFO hydrogel.

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“…The BPN-DFO hydrogel shows a faster degradation rate than the hydrogel, and the possible cause is that BPN can improve the hydrophilicity of the components. 44 …”

Section: Discussionmentioning

confidence: 99%

A Composite Deferoxamine/Black Phosphorus Nanosheet/Gelatin Hydrogel Scaffold for Ischemic Tibial Bone Repair

Xu¹,

Gan²,

Wang³

et al. 2022

IJN

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“…The PPEK substrate was prepared by thermoforming and cut into small square pieces of 1 cm × 1 cm. Hexafluoroisopropanol solutions (10 mg/mL) of PPPEK50 and PPPEK100 were coated onto the surface of the PPEK substrate by spin coating at 2500 r/min for 60 s. 44 They were called P50/PPEK and P100/PPEK, respectively. The spin-coated samples were then dried and immersed in 1.5× SBF at 37 °C for 1, 3, and 7 days.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Phosphonate-Functionalized Poly(phthalazinone ether ketones) Induce the Formation of Apatite Coatings for Enhanced Biocompatibility and Osteogenic Activity

Cheng

et al. 2022

ACS Appl. Polym. Mater.

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Poly(phthalazinone ether ketone) (PPEK), a poly(aryl ether ketone) (PAEK), has good thermal stability, nitrogen-containing functional groups, and bone-like mechanical properties. However, its surface biological inertness limits its application in biomedical applications. This paper presents the synthesis of PPEK modified with quantitative introduction of phosphonate groups (PPPEK) by a simple wet chemical modification method. The biomineralization process is regulated by the number of phosphonate groups, and a coating similar to bone apatite is deposited on the surface of PPEK substrates, thus greatly improving the biocompatibility of the material surface. The chemical structures of PPPEK are confirmed by 1 H NMR and FTIR. Microstructural and compositional studies performed by SEM, TEM, XPS, and XRD reveal that apatite coating is significantly enhanced with the increase in the phosphonate groups on the PPEK substrate. In addition, cell culture studies show that apatite coating induced by PPPEK promoted cell viability, cell adhesion, and osteogenesis of MC3T3-E1 cells.

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“…The MTT method (Sangon Biotechnology Co. Ltd) was used for in vitro cytotoxicity assays. CCK-8 solution (Sangon Biotechnology Co. Ltd) was used to assess the viability of MC3T3-E1 cells co-cultured with each sample, and the detailed procedures were in the supporting information …”

Section: Methodsmentioning

confidence: 99%

“…CCK-8 solution (Sangon Biotechnology Co. Ltd) was used to assess the viability of MC3T3-E1 cells co-cultured with each sample, and the detailed procedures were in the supporting information. 40 2.7.3. ALP Activity and Calcium Deposition.…”

Section: Preparation Of Phosphonatementioning

confidence: 99%

Stabilization of Apatite Coatings on PPENK Surfaces by Mechanical Interlocking to Promote Bioactivity and Osseointegration In Vivo

Cheng

Yang

Liu

et al. 2022

ACS Appl. Mater. Interfaces

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Apatite coatings with high stability can effectively improve the surface bioactivity and osteogenic activity of implant materials. In clinical practice, the ability of apatite coatings to bond with the substrate is critical to the effect of implants. Here, we propose a strategy to construct a three-dimensional (3D) nanoporous structure on the surface of a poly(phthalazinone ether nitrile ketone) (PPENK) substrate and introduce a polydopamine (PDA) coating with grafted phosphonate groups to enhance the overall deposition of a bone-like apatite coating in the 3D nanoporous structure during mineralization. This method leads to a mechanical interlocking between the apatite coating and the substrate, which increases the stability of the apatite coating. The apatite coating confers a better bioactive surface to PPENK and also promotes osteogenic differentiation and adhesion of MC3T3-E1 osteoblasts in vitro. The samples are then implanted into rat femurs to characterize in vivo osseointegration. Micro-CT data and histological staining of tissue sections reveal that PPENK with a stable apatite coating induces less fibrous capsule formation and no inflammatory response and promotes osteogenic differentiation and bone-bonding strength. This enhances the long-term use of PPENK implant materials and shows great potential for clinical application as orthopedic implants.

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Enhancing Tissue Adhesion and Osteoblastic Differentiation of MC3T3‐E1 Cells on Poly(aryl ether ketone) by Chemically Anchored Hydroxyapatite Nanocomposite Hydrogel Coating

Cited by 12 publications

References 59 publications

A Composite Deferoxamine/Black Phosphorus Nanosheet/Gelatin Hydrogel Scaffold for Ischemic Tibial Bone Repair

A Composite Deferoxamine/Black Phosphorus Nanosheet/Gelatin Hydrogel Scaffold for Ischemic Tibial Bone Repair

Phosphonate-Functionalized Poly(phthalazinone ether ketones) Induce the Formation of Apatite Coatings for Enhanced Biocompatibility and Osteogenic Activity

Stabilization of Apatite Coatings on PPENK Surfaces by Mechanical Interlocking to Promote Bioactivity and Osseointegration In Vivo

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