Enhancement of the sensitivity of valacyclovir and acyclovir for their spectrofluorimetric determination in human plasma

Derayea, Sayed M.; Omar, Mahmoud A.; Mostafa, Islam M.; Hammad, Mohamed A.

doi:10.1039/c5ra11635j

Cited by 21 publications

(18 citation statements)

References 29 publications

(24 reference statements)

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“…This result indicates that the microenvironmental state surrounding the studied drugs totally differs from that in aqueous solution. This effect is attributed to confinements forced on rotational movements inside the micellar medium …”

Section: Resultssupporting

confidence: 86%

“…One mL standard drug working solution was mixed with 1 mL drug‐free plasma obtained from healthy human volunteers after centrifugation at 18 659 g for 30 min. Two mL of acetonitrile were added as protein precipitating agent, and the mixture was completed with methanol to 10 mL, and then centrifuged at 18 659 g for about 20 min. Two mL of the clear supernatant was diluted with distilled water as appropriate to cover the linearity range for all investigated drugs, and then the sample analyzed using a general analytical protocol.…”

Section: Methodsmentioning

confidence: 99%

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Micellar enhanced spectrofluorimetric approach for nanogram detection of certain α₁‐blocker drugs: Application in pharmaceutical preparations and human plasma

2018

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Alpha1-adrenergic-blocking drugs, namely; alfuzosin hydrochloride (ALF), doxazosin mesylate (DOX) and terazosin hydrochloride (TER) are effective as antihypertensive agents as well as in management of benign prostatic hypertrophy. In this study, a simple, very fast, highly sensitive and cheap technique was optimized for assay of these drugs in pure states and pharmaceutical tablets. The proposed method is dependent on enhancement of the native fluorescence of investigated drugs using the polyoxyethylene 50 stearate (POE50S) micellar system. The method showed excitation at 325, 340 and 250 nm for ALF, DOX and TER, respectively and an emission maxima at 382 nm. The fluorescence intensity-concentration charts of studied drugs were attained utilizing concentration ranges (2.0-60.0 ng mL ) for DOX and (4.0-100.0 ng mL ) for ALF and TER with quantitation limits 2.9, 1.6 and 2.5 ng mL for ALF, DOX and TER, respectively. The suggested technique was approved according to International Council for Harmonisation (ICH) standards and the United States Food and Drug Administration (US FDA) bioanalytical method validation and has been effectively applied for assay of these medications in their dosage forms as well as for content uniformity test. The developed procedure was also efficiently applied for determination of these drugs in real human plasma with high accuracy.

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Section: Resultssupporting

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Micellar enhanced spectrofluorimetric approach for nanogram detection of certain α₁‐blocker drugs: Application in pharmaceutical preparations and human plasma

2018

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“…The use of micellar media has become one technique that is commonly applied to enhance the fluorescence emission of many fluorophores and therefore could successfully decrease the limit of detection (LOD) of the method. This approach has been applied to improve the sensitivity of the spectrofluorimetric analysis of several compounds . As presented in Figure , SPV has weak inherent fluorescence in aqueous solution at λ em 427 nm after excitation at λ ex 337 nm.…”

Section: Resultsmentioning

confidence: 99%

Microenvironment improvement protocol for the sensitive spectrofluorimetric determination of an hepatitis C virus antiviral (Simeprevir): application to human plasma

et al. 2019

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Simeprevir (SPV) is a powerful antihepatitis C virus agent that was newly introduced into the pharmaceutical market. We here established and validated an easy, simple, and sensitive spectrofluorimetric method for its estimation at λ em 427 nm (λ ex 337 nm). The suggested procedure was based on two times enhancement in the original emission of SPV through modifying its microenvironment in buffered aqueous solution by adding Triton X-100. The relationship between the concentration of SPV and the observed fluorescence intensity was linear in the range 0.06-1.0 μg ml −1 with a correlation coefficient of 0.9997. The limits of detection and quantitation were 21 and 64 ng ml −1 , respectively. The present method was effectively applied to quantify SPV content in pharmaceutical tablets and human plasma spiked with the drug with no interference from tablet excipients or plasma components. K E Y W O R D Shuman plasma, pharmaceutical analysis, Simeprevir, spectrofluorimetry, surfactants

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“…Regarding the in vivo study; 120 mg of FBX tablet is absorbed orally with C max (5.273 ± 1.781 μg/mL) in 1.5 h . The following equation was used in calculating the percent recovery of the FBX in human plasma samples:

% recovery italicin vivo = delivery italicin vivo \times % recovery italicin vitro / delivery italicin vitro

…”

Section: Resultsmentioning

confidence: 99%

Fluorimetric determination of febuxostat in dosage forms and in real human plasma via Förster resonance energy transfer

2018

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A rapid, simple, selective and precise fluorimetric method was developed and validated for determination of a selective xanthine oxidase inhibitor; febuxostat (FBX) in pharmaceutical formulations and in human plasma. The proposed method is based on quenching effect of FBX on the fluorescence intensity of terbium (Tb ) through fluorescence resonance energy transfer (FRET) from Tb to FBX. The formed complex was measured at λ 320 nm/λ 490 nm against a reagent blank. Fluorescence intensity of Tb was diminished when FBX was added. A linear relationship between the fluorescence quenching value of the formed complex ΔF=FTb3+-FFBX-Tb3+ and the concentration of FBX was investigated. The reaction conditions and the fluorescence spectral properties of the complex have been studied. The linearity range of the developed method was 1.0-16.0 μg/ml. The suggested method was applied successfully for the estimation of FBX in bulk powder, dosage forms and spiked plasma samples with excellent recoveries (96.79-98.89%). In addition, the developed method has been successfully applied for determination of FBX in real plasma samples collected from healthy volunteers with good recoveries (82.06-85.65%). All obtained results of the developed method were statistically analyzed and validated according to ICH (International Conference on Harmonization) guidelines.

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Enhancement of the sensitivity of valacyclovir and acyclovir for their spectrofluorimetric determination in human plasma

Abstract: Two rapid, simple and highly sensitive spectrofluorimetric methods have been developed and validated for determination of valacyclovir hydrochloride (VAC) and acyclovir (ACV).

Cited by 21 publications

References 29 publications

Micellar enhanced spectrofluorimetric approach for nanogram detection of certain α₁‐blocker drugs: Application in pharmaceutical preparations and human plasma

Micellar enhanced spectrofluorimetric approach for nanogram detection of certain α₁‐blocker drugs: Application in pharmaceutical preparations and human plasma

Microenvironment improvement protocol for the sensitive spectrofluorimetric determination of an hepatitis C virus antiviral (Simeprevir): application to human plasma

Fluorimetric determination of febuxostat in dosage forms and in real human plasma via Förster resonance energy transfer

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Enhancement of the sensitivity of valacyclovir and acyclovir for their spectrofluorimetric determination in human plasma

Abstract: Two rapid, simple and highly sensitive spectrofluorimetric methods have been developed and validated for determination of valacyclovir hydrochloride (VAC) and acyclovir (ACV).

Cited by 21 publications

References 29 publications

Micellar enhanced spectrofluorimetric approach for nanogram detection of certain α1‐blocker drugs: Application in pharmaceutical preparations and human plasma

Micellar enhanced spectrofluorimetric approach for nanogram detection of certain α1‐blocker drugs: Application in pharmaceutical preparations and human plasma

Microenvironment improvement protocol for the sensitive spectrofluorimetric determination of an hepatitis C virus antiviral (Simeprevir): application to human plasma

Fluorimetric determination of febuxostat in dosage forms and in real human plasma via Förster resonance energy transfer

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Micellar enhanced spectrofluorimetric approach for nanogram detection of certain α₁‐blocker drugs: Application in pharmaceutical preparations and human plasma

Micellar enhanced spectrofluorimetric approach for nanogram detection of certain α₁‐blocker drugs: Application in pharmaceutical preparations and human plasma