Enhancement of freezing-resistance of Lactobacillus rhamnosus by the application of cell immobilization

Tsen, Jen-Horng; Huang, Huiying; King, V. An-Erl

doi:10.2323/jgam.53.215

Cited by 9 publications

(7 citation statements)

References 38 publications

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“…All solutions were tyndalized (Poliak et al 2008). Gel beads with the cells immobilized in them were obtained by ionotropic gelation (Tsen et al 2007). The bead diameter was 2000 ± 100 μm.…”

Section: Immobilizationmentioning

confidence: 99%

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Addition of disaccharides and dimethyl sulfoxide to sodium alginate gel improves viability of immobilized Saccharomyces boulardii after cryopreservation

Vysekantsev

Buriak

Martsenyuk

et al. 2021

Nova Biotechnol. Chim.

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Gel-immobilized microorganisms are increasingly used in microbiological industries. Currently, the problem of developing technologies for long-term storage of microorganisms immobilized in gel carriers remains urgent. Low-temperature storage is the most effective method of preserving microorganisms. The viability of immobilized cells is affected by cryoprotective properties of gel matrix as well as cooling regimes. Therefore, the effects of incorporation of cryoprotective agents in alginate gel and cooling regimes on the viability of immobilized Saccharomyces boulardii cells after cryopreservation were studied. Incorporation of non-permeable cryoprotectants (sucrose, lactose, and trehalose) and permeable one dimethyl sulfoxide (DMSO) in alginate gel beads promoted an increase in the viability of immobilized cells after freeze-thawing. The highest viability rates of the gel-immobilized cells were observed in the alginate gel beads incorporating combinations of DMSO (5 – 10 % v/v) and one of the disaccharides (10 – 20 % w/v). In all experiments, slow cooling provided significantly higher viability if compared to the rapid immersion of the samples into liquid nitrogen.

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Section: Immobilizationmentioning

confidence: 99%

“…It is logical that low temperatures and lyophilization can also be used to store the microorganisms immobilized in gel beads. Previously the results of such studies have been reported (Kearney et al 1990;Tsen et al 2007;Solanki et al 2013;Cagol et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Addition of disaccharides and dimethyl sulfoxide to sodium alginate gel improves viability of immobilized Saccharomyces boulardii after cryopreservation

Vysekantsev

Buriak

Martsenyuk

et al. 2021

Nova Biotechnol. Chim.

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“…Клітини B.bifidum іммобілізували в альгінатному гелі методом йонотранспортного гелеутворення в модифікації [8].…”

Section: матеріали та методи дослідженняunclassified

Impact of Hypothermal and Low Temperature Storage Conditions on Viability of Immobilized Probiotics Bifidobacterium Bifidum

Ananina

Stepanyuk

Vysekantsev

et al. 2020

Act. Probl. of the Modern Med.

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Dysbiosis is among the challenges the healthcare is facing now. This condition results from to the combined impact of a number of negative factors on the human body, and immune status weakening. The priority of current biotechnology is the elaboration of probiotics immobilized in gel particles that can be used to correct dysbiosis. Immobilization of probiotic cells in alginate gel granules protects them from the damaging effects of barrier functions of the gastrointestinal tract. The aim of the study was to investigate the effect of hypothermic and low-temperature storage on the viability of the probiotic Bifidobacterium bifidum immobilized in granules of alginate gel. Bacterial cells were immobilized in granules of unmodified gel (1% sodium alginate solution) and in granules of modified sucrose-milk-lactose gel. Granules with immobilized bacterial cells were placed into cryotubes. The samples kept at temperatures of +4, -12, -20, -80 and -196 ° C for 12 months (study period). It was found that storage at temperatures -80 and -196°C provided high viability of the probiotic throughout the study. During storage at temperatures of +4 and -12 ° C, the death of bacteria was observed in 1 – 3 months, depending on the composition of the gel particles, and at a temperature of -20°C the death occurs in 6-9 months. Conclusions: It has been established that the viability of B. bifidum culture cells immobilized in alginate gel granules is influenced by both storage temperature modes and gel modification by the introduction of sucrose-milk-lactose medium. At temperature ragnes of -80 and -196°C, immobilized bacteria do not die during the year (observation period). When storing at the temperatures +4, -12, -20°C, higher viability rates and longer shelf life of bifidobacterial cells were observed in samples of alginate gel granules modified with sucrose-milk-lactose medium. The study has shown that being kept at the low temperature values, B. bifida cells immobilized in unmodified and modified with sucrose-milk-lactose medium do not change their cultural and morphological properties.

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“…Process engineering utilizes bioengineering principles to change metabolic pathways or biological transformation processes to improve the resistance of microorganisms in the production process. Concretely, the resistance can be improved by the exogenous addition of inducers , osmotic vaporization, and cell immobilization . Some examples of improving the stress resistance of microorganisms using gene process engineering are listed in Table .…”

Section: Process Engineering To Improve Microbial Stress Resistancementioning

confidence: 99%

“…Exogenous inducer substances can activate or inhibit biochemical reactions involved in metabolic pathways by causing metabolites and enzymes in the cells to be altered. The exogenous addition was shown to have protective effects on microorganisms in response to high osmotic pressure, for example, glutathione (GSH) , myocardial phosphatide , dihydroxy acetone , a mixture of glucose and β‐disaccharide, and lignocellulosic residues .…”

Section: Process Engineering To Improve Microbial Stress Resistancementioning

confidence: 99%

Techniques for enhancing the tolerance of industrial microbes to abiotic stresses: A review

Zhou

Zhang

et al. 2019

Biotech and App Biochem

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The diversity of stress responses and survival strategies evolved by microorganism enables them to survive and reproduce in a multitude of harsh environments, whereas the discovery of the underlying resistance genes or mechanisms laid the foundation for the directional enhancement of microbial tolerance to abiotic stresses encountered in industrial applications. Many biological techniques have been developed for improving the stress resistance of industrial microorganisms, which greatly benefited the bacteria on which industrial production is based. This review introduces the main techniques for enhancing the resistance of microorganisms to abiotic stresses, including evolutionary engineering, metabolic engineering, and process engineering, developed in recent years. In addition, we also discuss problems that are still present in this area and offer directions for future research.

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Enhancement of freezing-resistance of Lactobacillus rhamnosus by the application of cell immobilization

Cited by 9 publications

References 38 publications

Addition of disaccharides and dimethyl sulfoxide to sodium alginate gel improves viability of immobilized Saccharomyces boulardii after cryopreservation

Addition of disaccharides and dimethyl sulfoxide to sodium alginate gel improves viability of immobilized Saccharomyces boulardii after cryopreservation

Impact of Hypothermal and Low Temperature Storage Conditions on Viability of Immobilized Probiotics Bifidobacterium Bifidum

Techniques for enhancing the tolerance of industrial microbes to abiotic stresses: A review

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