Techniques for enhancing the tolerance of industrial microbes to abiotic stresses: A review

Lv, Ziyao; Zhou, Jie; Zhang, Yue; Zhou, Xue; Xu, Ning; Xin, Fengxue; Ma, Jiangfeng; Jiang, Min; Dong, Weiliang

doi:10.1002/bab.1794

Cited by 2 publications

(2 citation statements)

References 59 publications

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“…However, one way to improve osmotolerance of microorganisms is through targeting genes involved in responses to increased osmotic pressure through metabolic engineering and has become an intensive research approach [ 23 ]. Recently, C. bescii was investigated to identify its response mechanism to higher osmolarities, which then can be targeted by directed engineering [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization and adaptation of Caldicellulosiruptor strains to higher sugar concentrations, targeting enhanced hydrogen production from lignocellulosic hydrolysates

Byrne

Björkmalm

Bostick

et al. 2021

Biotechnol Biofuels

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Background The members of the genus Caldicellulosiruptor have the potential for future integration into a biorefinery system due to their capacity to generate hydrogen close to the theoretical limit of 4 mol H2/mol hexose, use a wide range of sugars and can grow on numerous lignocellulose hydrolysates. However, members of this genus are unable to survive in high sugar concentrations, limiting their ability to grow on more concentrated hydrolysates, thus impeding their industrial applicability. In this study five members of this genus, C.owensensis, C. kronotskyensis, C.bescii, C.acetigenus and C.kristjanssonii, were developed to tolerate higher sugar concentrations through an adaptive laboratory evolution (ALE) process. The developed mixed population C.owensensis CO80 was further studied and accompanied by the development of a kinetic model based on Monod kinetics to quantitatively compare it with the parental strain. Results Mixed populations of Caldicellulosiruptor tolerant to higher glucose concentrations were obtained with C.owensensis adapted to grow up to 80 g/L glucose; other strains in particular C. kristjanssonii demonstrated a greater restriction to adaptation. The C.owensensis CO80 mixed population was further studied and demonstrated the ability to grow in glucose concentrations up to 80 g/L glucose, but with reduced volumetric hydrogen productivities ($$Q_{{{\text{H}}_{2} }}$$ Q H 2 ) and incomplete sugar conversion at elevated glucose concentrations. In addition, the carbon yield decreased with elevated concentrations of glucose. The ability of the mixed population C.owensensis CO80 to grow in high glucose concentrations was further described with a kinetic growth model, which revealed that the critical sugar concentration of the cells increased fourfold when cultivated at higher concentrations. When co-cultured with the adapted C.saccharolyticus G5 mixed culture at a hydraulic retention time (HRT) of 20 h, C.owensensis constituted only 0.09–1.58% of the population in suspension. Conclusions The adaptation of members of the Caldicellulosiruptor genus to higher sugar concentrations established that the ability to develop improved strains via ALE is species dependent, with C.owensensis adapted to grow on 80 g/L, whereas C.kristjanssonii could only be adapted to 30 g/L glucose. Although C.owensensis CO80 was adapted to a higher sugar concentration, this mixed population demonstrated reduced $$Q_{{{\text{H}}_{2} }}$$ Q H 2 with elevated glucose concentrations. This would indicate that while ALE permits adaptation to elevated sugar concentrations, this approach does not result in improved fermentation performances at these higher sugar concentrations. Moreover, the observation that planktonic mixed culture of CO80 was outcompeted by an adapted C.saccharolyticus, when co-cultivated in continuous mode, indicates that the robustness of CO80 mixed culture should be improved for industrial application.

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Section: Introductionmentioning

confidence: 99%

Characterization and adaptation of Caldicellulosiruptor strains to higher sugar concentrations, targeting enhanced hydrogen production from lignocellulosic hydrolysates

Byrne

Björkmalm

Bostick

et al. 2021

Biotechnol Biofuels

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show abstract

“…Lv et al. [12] discuss strategies, including rational and evolutionary pathway engineering, for developing strains with increased tolerance to industrial environments.…”

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confidence: 99%

Editorial for the special issue on “Metabolic engineering”

Mazzoli

Liu

2020

Biotech and App Biochem

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Techniques for enhancing the tolerance of industrial microbes to abiotic stresses: A review

Cited by 2 publications

References 59 publications

Characterization and adaptation of Caldicellulosiruptor strains to higher sugar concentrations, targeting enhanced hydrogen production from lignocellulosic hydrolysates

Characterization and adaptation of Caldicellulosiruptor strains to higher sugar concentrations, targeting enhanced hydrogen production from lignocellulosic hydrolysates

Editorial for the special issue on “Metabolic engineering”

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