Enhancement of aflatoxin B1-induced enzyme altered hepatic foci in rats by treatment with carbon tetrachloride

Abstract

The effect of carbon tetrachloride (CC1 4 ) on aflatoxin B 1 (AFB 1 )-induced enzyme altered hepatic foci has been examined in young male Fischer rats given AIN-76A diet. A single i.p. dose of AFB 1 (0.2 mg/kg body wt) was given to rats 24 h after partial hepatectomy. Two weeks later, CCl 4 (0.8 ml/kg body wt) was injected i.p. once a week for 9 weeks. Animals were sacrificed 24 h after the last dose of CC1 4 and glutathione S-transferase placental form (GST-P) and -glutamyl transpeptidase (GGT) positive hepatic foci were analyzed by immunohistochemical and histochemical methods, respectively. Ten weeks after A F B 1 dosing, treatment with CCl 4 increased the number of AFB 1 -induced enzyme altered foci several fold and produced a ten to twenty-fold increase in area and volume. GST-P was more sensitive than GGT in detecting AFB 1 -i n d u c e d enzyme altered foci. Treatment with AFB 1 or CCl 4 produced mild hepatic fibrosis in zones 1 and 3 r e s p e c t i v e l y, whereas both treatments produced severe fibrosis in zones 1 to 3 areas. Tr e a t -ment with CCl 4 after AFB 1 dosing lowered hepatic GSH levels by 20% and increased lipid peroxidation by 40%. It appears that CCl 4 , by being an effective enhancer of AFB 1 -induced enzyme altered hepatic foci in the rat, may mimic cirrhosis observed in human hepatocellular carcinoma.

In vivo antitumour potential of camel’s milk against hepatocellular carcinoma in rats and its improvement of cisplatin renal side effects

“…Placental glutathione- S -transferases P-GST has been reported as a sensitive marker for preneoplastic hepatic foci during chemical hepatocarcinogenesis in rats (Qin et al. 1998 ; Guo et al. 2000 ).…”

Bifidobacterium breve alleviates gut–liver-axis injury caused by high-fat diet and aflatoxin B1 in mice

“…each day. The dose of AFB1 exposure was selected according to previous studies [20][21][22] . Dimethylsulfoxide was used as the vehicle solvent.…”