Enhancement of adaptive response in peanut hairy root by exogenous signalling molecules under cadmium stress

Pilaisangsuree, Vijakhana; Anuwan, Piyanuch; Supdensong, Kanitha; Lumpa, Pimpimon; Kongbangkerd, Anupan; Limmongkon, Apinun

doi:10.1016/j.jplph.2020.153278

Cited by 18 publications

(7 citation statements)

References 31 publications

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“…Our previous study investigated different combinations of abiotic elicitors, such as PQ, Cd, MeJA, and CD, in peanut hairy root culture, and the results demonstrated that all these elicitors, especially the combination and sequential elicitor treatment, could induce diverse amounts of stilbene compound production. The induction of trans-resveratrol was not much different; however, the prenylated stilbenes of transarachidin-1 and trans-arachidin-3 showed greater diversity in amounts between each combination of elicitors (Pilaisangsuree et al 2020;Somboon et al 2019;Wongshaya et al 2020).…”

Section: Discussionmentioning

confidence: 95%

“…Our previous research on elicitation with the plant herbicide PQ demonstrated that elicitation of PQ prior to the combination of MeJA + CD, PQ_(MeJA + CD), resulted in the highest amounts of transarchidin-1 and trans-arachidin-3, with 178 ± 0.05 and 444 ± 0.06 mg/g dry weight of hairy roots, respectively (Somboon et al 2019). In the study of elicitation with heavy metal Cd, the treatment of MeJA + CD prior to Cd, (MeJA + CD)_Cd, represented the highest amounts of trans-arachidin-1 and transarachidin-3, with only 9.67 ± 6.67 and 78.84 ± 32.60 mg/g dry weight of hairy roots, respectively (Pilaisangsuree et al 2020). The present study concerning the treatment involving a combination of a non-toxic CHT elicitor (CHT + MeJA + CD) illustrates the most promising results, with the highest amounts of trans-arachidin-1 (684.30 ± 183.85 mg/g dry weight of hairy roots) and trans-arachidin-3 (543.94 ± 171.17 mg/g dry weight of hairy roots) in a 72-h elicitation period.…”

Section: Discussionmentioning

confidence: 99%

“…Yang et al (2015) reported that the combination of MeJA and CD can be a good elicitor to induce trans-resveratrol, trans-arachidin-1, and trans-arachidin-3 with high antioxidant activity in peanut hairy root culture. Our previous studies investigated the different sequential elicitation approaches of MeJA plus CD treatment before or after the application of paraquat (PQ) or cadmium (Cd), and the results demonstrated that the sequential elicitation strategy could evoke high levels of stilbene compounds with high biological activity in peanut hairy root culture (Pilaisangsuree et al 2020;Somboon et al 2019). ).…”

Section: Introductionmentioning

confidence: 99%

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Using a combination of chitosan, methyl jasmonate, and cyclodextrin as an effective elicitation strategy for prenylated stilbene compound production in Arachis hypogaea L. hairy root culture and their impact on genomic DNA

Chayjarung

Poonsap

Pankaew

et al. 2021

Plant Cell Tiss Organ Cult

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Peanut (Arachis hypogaea L.) hairy roots are a potential tool for stilbene compound production. This study focuses on the most e cient elicitation strategy for inducing stilbene compound production, especially for the prenylated forms of trans-arachidin-1 and trans-arachidin-3. To the best of our knowledge, this is the rst report of simultaneous treatment involving chitosan (CHT), methyl jasmonate (MeJA), and cyclodextrin (CD); CHT+MeJA+CD in peanut hairy roots to induce substantial amounts of trans-arachidin-1 and trans-arachidin-3 at 72 h of the elicitation period. The results demonstrate the highest amounts of trans-arachidin-1 and trans-arachidin-3, with 684.30 ± 183.85 and 543.94 ± 171.17 mg/g dry weight of hairy roots, respectively. The highest antioxidant capacity determined by ABTS and FRAP assays was 188.95 ± 0.43 µmol Trolox/g dry weight of hairy roots and 24.61 ± 0.50 mg ascorbic acid/g dry weight of hairy roots, respectively, while the highest phenolic content was also detected in this crude extract, with 8.67 ± 0.11 mg gallic acid/g dry weight of hairy roots. The antioxidant and pro-oxidant activity of the CHT+MeJA+CD crude extract in the protection and damage of DNA is of great interest and can have major positive impacts on health promotion and disease prevention. Key MessageChitosan, methyl jasmonate, and cyclodextrin induce high stilbene compounds. The antioxidant in crude extract could scavenge free radicals and protect DNA, while pro-oxidant activity causes severe damage to genomic DNA.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Using a combination of chitosan, methyl jasmonate, and cyclodextrin as an effective elicitation strategy for prenylated stilbene compound production in Arachis hypogaea L. hairy root culture and their impact on genomic DNA

Chayjarung

Poonsap

Pankaew

et al. 2021

Plant Cell Tiss Organ Cult

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“…Previously, the ethanolic extract of peanut sprouts rich in stilbenoids, such as resveratrol, arachidin-1, and arachidin-3, showed antioxidant and anti-inflammatory activities [23]. The antioxidant activity of stilbenoid-rich extract have been previously reported from peanut hairy roots treated with paraquat, MeJA, and CD and peanut hairy roots treated with cadmium, MeJA, and CD [24,25]. In this study, the IC 50 value of the stilbenoid-rich extract of peanut hairy roots was lower in comparison to the phenolic extract of grape pomace from five different red grape cultivars with IC 50 values ranging from 14.45 µg/mL to 38.93 µg/mL suggesting higher antioxidant properties of peanut hairy root extracts [26].…”

Section: Comparison Of Antioxidant Activity Of Stilbenoid-rich Extract From Hairy Roots Of Three Peanut Cultivarsmentioning

confidence: 98%

Antioxidant Assessment of Prenylated Stilbenoid-Rich Extracts from Elicited Hairy Root Cultures of Three Cultivars of Peanut (Arachis hypogaea)

2021

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Peanut produces prenylated stilbenoids upon biotic stress. However, the role of these compounds against oxidative stress have not been thoroughly elucidated. To this end, the antioxidant capacity of extracts enriched in prenylated stilbenoids and derivatives was studied. To produce these extracts, hairy root cultures of peanut cultivars Hull, Tifrunner, and Georgia Green were co-treated with methyl jasmonate, cyclodextrin, hydrogen peroxide, and magnesium chloride and then the stilbenoids were extracted from the culture medium. Among the three cultivars, higher levels of the stilbenoid derivatives arachidin-1 and arachidin-6 were detected in cultivar Tifrunner. Upon reaction with 2,2-diphenyl-1picrylhydrazyl, extracts from cultivar Tifrunner showed the highest antioxidant capacity with an IC50 of 6.004 µg/mL. Furthermore, these extracts had significantly higher antioxidant capacity at 6.25 µg/mL and 3.125 µg/mL when compared to extracts from cultivars Hull and Georgia Green. The stilbenoid-rich extracts from peanut hairy roots show high antioxidant capacity and merit further study as potential nutraceuticals to promote human health.

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“…Tobacco hairy roots could also be used as a convenient system for studying the function of genes in circadian rhythm [8]. Similarly, a tobacco hairy root culture system was established to investigate the function of genes related to aluminum [9] or cadmium tolerance [10]. Considering the culturing time needed, induction of hairy root by R. rhizogenes has many advantages over traditional transgenic technology in the study of gene function and regulation of secondary metabolism [9].…”

Section: Introductionmentioning

confidence: 99%

An Optimized Tobacco Hairy Root Induction System for Functional Analysis of Nicotine Biosynthesis-Related Genes

et al. 2022

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Rhizobium rhizogenes-mediated plant hairy root induction is a convenient method for functional study of root-specific genes. To develop an optimized tobacco hairy root induction system and study gene function in nicotine biosynthesis, we investigated hairy root induction by three R. rhizogenes strains, R1601, K599, and LBA9402, on different media with leaf discs from plants of different ages, and we observed that the strain LBA9402 used for explant infection exhibited the highest hairy root induction rate with 4 and 8 week old leaf discs of the tobacco ‘Coker176’ on 2/3MS medium, and it could also be used as a cargo delivering foreign genes to hairy roots. Overexpression of MsSPL12 gene, an alfalfa (Medicago sativa) SQUAMOSA promoter binding protein-like (SPL) transcription factor, significantly improved nicotine production in transgenic hairy roots, reaching 1.38–1.85 mg/g compared to 0.5 mg/g of the controls. Expression analysis of the nicotine biosynthesis and transport-related genes responding to methyl-jasmonate (MeJA) treatment revealed a significant upregulation of NtMPO2 responsible for increased nicotine biosynthesis in MsSPL12 transgenic hairy roots. Our results establish a high-throughput approach for gene functional characterization in the hairy roots of a tobacco elite cultivar, ‘Coker176’, as well as suggest a system for efficiently manipulating tobacco nicotine biosynthesis.

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Enhancement of adaptive response in peanut hairy root by exogenous signalling molecules under cadmium stress

Cited by 18 publications

References 31 publications

Using a combination of chitosan, methyl jasmonate, and cyclodextrin as an effective elicitation strategy for prenylated stilbene compound production in Arachis hypogaea L. hairy root culture and their impact on genomic DNA

Using a combination of chitosan, methyl jasmonate, and cyclodextrin as an effective elicitation strategy for prenylated stilbene compound production in Arachis hypogaea L. hairy root culture and their impact on genomic DNA

Antioxidant Assessment of Prenylated Stilbenoid-Rich Extracts from Elicited Hairy Root Cultures of Three Cultivars of Peanut (Arachis hypogaea)

An Optimized Tobacco Hairy Root Induction System for Functional Analysis of Nicotine Biosynthesis-Related Genes

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