Antioxidant Assessment of Prenylated Stilbenoid-Rich Extracts from Elicited Hairy Root Cultures of Three Cultivars of Peanut (Arachis hypogaea)

Abstract

Peanut produces prenylated stilbenoids upon biotic stress. However, the role of these compounds against oxidative stress have not been thoroughly elucidated. To this end, the antioxidant capacity of extracts enriched in prenylated stilbenoids and derivatives was studied. To produce these extracts, hairy root cultures of peanut cultivars Hull, Tifrunner, and Georgia Green were co-treated with methyl jasmonate, cyclodextrin, hydrogen peroxide, and magnesium chloride and then the stilbenoids were extracted from the culture medium. Among the three cultivars, higher levels of the stilbenoid derivatives arachidin-1 and arachidin-6 were detected in cultivar Tifrunner. Upon reaction with 2,2-diphenyl-1picrylhydrazyl, extracts from cultivar Tifrunner showed the highest antioxidant capacity with an IC50 of 6.004 µg/mL. Furthermore, these extracts had significantly higher antioxidant capacity at 6.25 µg/mL and 3.125 µg/mL when compared to extracts from cultivars Hull and Georgia Green. The stilbenoid-rich extracts from peanut hairy roots show high antioxidant capacity and merit further study as potential nutraceuticals to promote human health.

Production and Secretion of Isowighteone in Hairy Root Cultures of Pigeon Pea (Cajanus cajan) Co-Treated with Multiple Elicitors

“…Notably, after 96 h of elicitation treatment, the color of the medium started to change from clear to yellowish, suggesting the induction and accumulation of prenylated phenolics in the culture medium. A similar yellow color was observed when peanut hairy root cultures were co-elicited to produce the prenylated stilbenoids arachidin-1 and arachidin-3 [ 28 ]. Based on these observations, the culture medium was initially analyzed for the presence of CSA.…”

“…Based on previous elicitation studies with prenylated stilbenoids [ 20 , 28 ] and the above experiment, 12-day-old hairy root cultures (mid-log stage) were selected and elicited for different time periods, i.e., 48 h, 96 h, 144 h, and 168 h. The yellowish color of the medium increased gradually with the elicitation time and the highest intensity was observed at 168 h ( Figure 5 A). HPLC analysis of the ethyl acetate extract from the culture medium showed a higher accumulation of CSA as the elicitor treatment time increased to 144 h ( Figure 5 B).…”

Heterologous biosynthesis of prenylated resveratrol through multiplex metabolic engineering in Escherichia coli

“…S10 †). [39][40][41] However, the preparation of these cultures is rather intricate and unsuitable for scaled-up production. No 2-C-prenylated stilbenoids were produced in these cultures.…”

Elicitation of Stilbenes and Benzofuran Derivatives in Hairy Root Cultures of White Mulberry (Morus alba)

“…The latter co-treatment produced 4-fold and 2.5-fold increase in yield of the prenylated stilbenoids arachidin-1 and arachidin-3, respectively, compared to using CD alone as a elicitor [ 40 ]. Overall, enhanced production of prenylated stilbenoids was observed in peanut hairy root cultures co-treated with CD, MeJA, H 2 O 2 , and MgCl 2 [ 40 , 50 , 51 ]. Similarly, pigeon pea hairy root cultures co-treated with CD, MeJA, H 2 O 2 , and MgCl 2 showed high production of the bioactive prenylated flavonoid isowighteone [ 52 ].…”